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Nano Research 2023, 16(3): 4004-4012 https://doi.org/10.1007/s12274-022-5226-1
Research Article | Issue | Published: 07 December 2022

A bioactive nanocomposite sponge for simultaneous hemostasis and antimicrobial therapy

Show Author's Information Jiani Lei1,§,  Shanshan Li1,§,  Shuang Liu1,  Qingyuan Wu1,  Bolong Xu1,  Zhijun Huang2,  Nier Wu3,  Xiaolu Xiong3,  Huiyu Liu1( ),  Dongsheng Zhou3( )
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic–Inorganic Composites, Bionanomaterials & Translational Engineering Laboratory, Beijing Key Laboratory of Bioprocess, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China

§ Jiani Lei and Shanshan Li contributed equally to this work.

Keywords:
antibacterial, hemostasis, calcium peroxide, coagulation mechanism, composite sponge
Topics:
Medical applications
Cite this article:
Lei J, Li S, Liu S, et al. A bioactive nanocomposite sponge for simultaneous hemostasis and antimicrobial therapy. Nano Research, 2023, 16(3): 4004-4012. https://doi.org/10.1007/s12274-022-5226-1
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Abstract Full text Electronic supplementary material About this article

Uncontrollable bleeding and bacterial infections are the major reasons for the high mortality of post-traumatic. In this study, a composite hemostatic chitosan sponge CaO2@SiO2/CS was prepared by combining a novel core–shell inorganic nano hemostatic CaO2@SiO2 nanoparticles with carboxylated chitosan, which presents a multi-layered structure with a rough and hydrophilic surface for rapid absorption of blood. When the CaO2@SiO2 nanoparticles in the CaO2@SiO2/CS come into contact with blood, the silanol group on its surface and the released H2O2 and Ca2+ can recruit and activate platelets, while generating fibrin clots and activating the endo-exogenous coagulation cascade reaction to achieve rapid clotting. The H2O2 released from CaO2@SiO2 shows the antimicrobial capacity and stimulates the production of tissue factors by endothelial cells. Meanwhile, the silica coating reduces the cytotoxicity of bare CaO2, thus reducing the risk of secondary bleeding at the site of vascular injury. CaO2@SiO2/CS (48 s) showed a 1.83- and 2.52-fold reduction in hemostasis time compared to commercial gelfoam and CS in a femoral artery hemorrhage model. This study illustrates the hemostatic mechanism of CaO2@SiO2 and provides a reference for the development of clinical biomedical inorganic hemostatic materials.


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Abstract
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Electronic supplementary material
About this article

A bioactive nanocomposite sponge for simultaneous hemostasis and antimicrobial therapy

Show Author's information Jiani Lei1,§, Shanshan Li1,§, Shuang Liu1, Qingyuan Wu1, Bolong Xu1, Zhijun Huang2, Nier Wu3, Xiaolu Xiong3, Huiyu Liu1( ), Dongsheng Zhou3( )
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic–Inorganic Composites, Bionanomaterials & Translational Engineering Laboratory, Beijing Key Laboratory of Bioprocess, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China

§ Jiani Lei and Shanshan Li contributed equally to this work.

Abstract

Uncontrollable bleeding and bacterial infections are the major reasons for the high mortality of post-traumatic. In this study, a composite hemostatic chitosan sponge CaO2@SiO2/CS was prepared by combining a novel core–shell inorganic nano hemostatic CaO2@SiO2 nanoparticles with carboxylated chitosan, which presents a multi-layered structure with a rough and hydrophilic surface for rapid absorption of blood. When the CaO2@SiO2 nanoparticles in the CaO2@SiO2/CS come into contact with blood, the silanol group on its surface and the released H2O2 and Ca2+ can recruit and activate platelets, while generating fibrin clots and activating the endo-exogenous coagulation cascade reaction to achieve rapid clotting. The H2O2 released from CaO2@SiO2 shows the antimicrobial capacity and stimulates the production of tissue factors by endothelial cells. Meanwhile, the silica coating reduces the cytotoxicity of bare CaO2, thus reducing the risk of secondary bleeding at the site of vascular injury. CaO2@SiO2/CS (48 s) showed a 1.83- and 2.52-fold reduction in hemostasis time compared to commercial gelfoam and CS in a femoral artery hemorrhage model. This study illustrates the hemostatic mechanism of CaO2@SiO2 and provides a reference for the development of clinical biomedical inorganic hemostatic materials.

Keywords: antibacterial, hemostasis, calcium peroxide, coagulation mechanism, composite sponge

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Publication history
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Acknowledgements

Publication history

Received: 15 August 2022
Revised: 10 October 2022
Accepted: 19 October 2022
Published: 07 December 2022
Issue date: March 2023

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

This work was supported by the National Key Research and Development Program of China (No. 2021YFC2102900), the National Natural Science Foundation of China (Nos. U21A2085 and 22061130205), the Fundamental Research Funds for the Central Universities and Research Projects on Biomedical Transformation of China-Japan Friendship Hospital (No. XK2022-08), and the open Foundation of State Key Laboratory of Organic–Inorganic Composites, Beijing University of Chemical Technology (No. OIC-202201010).

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