As an important neurotransmitter, the detection of dopamine (DA) is of great significance for the diagnosis and treatment of neurological diseases. In this study, WO3-SnO2 nanoflake arrays were synthesized on fluorine-doped tin oxide (FTO) by hydrothermal synthesis and pulse electrodeposition, revealing significant surface-enhanced Raman scattering (SERS) activity with an enhancement factor (EF) reaching 4.79 × 107. The obvious EF was mainly ascribed to the charge transfer between WO3-SnO2 and methylene blue (MB) based on chemical mechanism (CM) and the molecular resonance effect. With the competitive adsorption of DA and absorbed MB, we prepared a SERS and electrochemical (EC) dual-mode detection platform of DA based on the WO3-SnO2 nanoflake arrays. The linear range (LR) was 5.00–1.75 × 103 nmol/L, and the detection limits (LODs) were as low as 1.50 and 0.80 nmol/L by SERS and EC respectively. Besides, the developed detection platform can shield the interference of many neurotransmitters similar to DA, showing good selectivity and excellent stability. In general, the SERS-EC dual-mode detection platform can be well applied to the detection of DA in cell lysate, demonstrating great potential in diagnosis of neurodegenerative diseases.
The authors are greatly grateful for the financial support from the National Natural Science Foundation of China (No. 21827814 for Y. T., No. 21974049 for T. T. Z.). Besides, this work was funded by Shanghai Rising-star Program (No. 20QA1403300) and Innovation Program of Shanghai Municipal Education Commission (No. 201701070005E00020).
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