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Nano Research 2023, 16(1): 999-1008 https://doi.org/10.1007/s12274-022-4837-x
Research Article | Issue | Published: 20 September 2022

Cationic antibacterial metal nanoclusters with traceable capability for fluorescent imaging the nano–bio interactions

Show Author's Information Yixiao Li1,  Shaohua Qu1,  Yumeng Xue1,  Lianbing Zhang2,  Li Shang1,3( )
School of Materials Science and Engineering, Northwestern Polytechnical University, Xi’an 710072, China
School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China
Northwestern Polytechnical University Chongqing Technology Innovation Center, Chongqing 400000, China
Keywords:
metal nanoclusters, antimicrobial agents, bacterial imaging, near-infrared fluorescence
Topics:
Nano biology
Cite this article:
Li Y, Qu S, Xue Y, et al. Cationic antibacterial metal nanoclusters with traceable capability for fluorescent imaging the nano–bio interactions. Nano Research, 2023, 16(1): 999-1008. https://doi.org/10.1007/s12274-022-4837-x
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Abstract Full text Electronic supplementary material About this article

A thorough understanding of antimicrobial mechanism is of great importance for developing novel, efficient antibacterial agents. While cationic nanoparticles, such as metal nanoclusters (NCs), represent an attractive type of antibacterial nanoagents, their interactions with bacteria remains largely un-elucidated. Herein, we report the synthesis of cationic bovine serum albumin-protected AuAgNCs (cBSA-AuAgNCs), which exhibit both near-infrared (NIR) fluorescence properties and significant antimicrobial effects. With E. coli and S. aureus as the representative bacteria, we investigated the antimicrobial process of cBSA-AuAgNCs in real-time based on their intrinsic fluorescence properties via fluorescence imaging. Our results showed that these cBSA-AuAgNCs exert their antimicrobial effects primarily by attaching on the outer membrane of bacteria without obvious internalization, which is significantly different from the antibacterial process of negatively-charged metal NCs. Further mechanistic investigation showed that these cationic NCs will cause serious disruption to the bacterial membrane due to strong electrostatic interactions, which then leads to over accumulation of reactive oxygen species (ROS) that finally causes the bactericidal action. This study demonstrates the great potential of cationic luminescent metal NCs as novel, traceable antimicrobial agents, which also provides new tools for further understanding microbial interactions of nanomedicines.


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Electronic supplementary material
About this article

Cationic antibacterial metal nanoclusters with traceable capability for fluorescent imaging the nano–bio interactions

Show Author's information Yixiao Li1, Shaohua Qu1, Yumeng Xue1, Lianbing Zhang2, Li Shang1,3( )
School of Materials Science and Engineering, Northwestern Polytechnical University, Xi’an 710072, China
School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China
Northwestern Polytechnical University Chongqing Technology Innovation Center, Chongqing 400000, China

Abstract

A thorough understanding of antimicrobial mechanism is of great importance for developing novel, efficient antibacterial agents. While cationic nanoparticles, such as metal nanoclusters (NCs), represent an attractive type of antibacterial nanoagents, their interactions with bacteria remains largely un-elucidated. Herein, we report the synthesis of cationic bovine serum albumin-protected AuAgNCs (cBSA-AuAgNCs), which exhibit both near-infrared (NIR) fluorescence properties and significant antimicrobial effects. With E. coli and S. aureus as the representative bacteria, we investigated the antimicrobial process of cBSA-AuAgNCs in real-time based on their intrinsic fluorescence properties via fluorescence imaging. Our results showed that these cBSA-AuAgNCs exert their antimicrobial effects primarily by attaching on the outer membrane of bacteria without obvious internalization, which is significantly different from the antibacterial process of negatively-charged metal NCs. Further mechanistic investigation showed that these cationic NCs will cause serious disruption to the bacterial membrane due to strong electrostatic interactions, which then leads to over accumulation of reactive oxygen species (ROS) that finally causes the bactericidal action. This study demonstrates the great potential of cationic luminescent metal NCs as novel, traceable antimicrobial agents, which also provides new tools for further understanding microbial interactions of nanomedicines.

Keywords: metal nanoclusters, antimicrobial agents, bacterial imaging, near-infrared fluorescence

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Publication history
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Acknowledgements

Publication history

Received: 13 June 2022
Revised: 28 July 2022
Accepted: 30 July 2022
Published: 20 September 2022
Issue date: January 2023

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

This work was supported by the National Youth Talents Programme, the Natural Science Foundation of Chongqing (No. cstc2021jcyj-msxmX0980), and the Research Fund of the State Key Laboratory of Solidification Processing (NPU, No. 2020-QZ-01).

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