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Nano Research 2022, 15(10): 9298-9308 https://doi.org/10.1007/s12274-022-4815-3
Research Article | Issue | Published: 26 August 2022

Antibacterial mechanism and transcriptomic analysis of a near-infrared triggered upconversion nanoparticles@AgBiS2 for synergetic bacteria-infected therapy

Show Author's Information Shi Chen1,§,  Zhaoyou Chu2,§,  Limian Cao1,§,  Lingling Xu2( ),  Qianqian Jin2,  Nian Liu1,  Benjin Chen2,  Ming Fang1,  Wanni Wang2,  Haisheng Qian2( ),  Min Shao1( )
Department of Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China

§ Shi Chen, Zhaoyou Chu, and Limian Cao contributed equally to this work.

Keywords:
photodynamic therapy, photothermal therapy, anti-methicillin-resistant Staphylococcus aureus (MRSA), RNA-sequencing, transcriptomic analysis
Topics:
Bacteria
Cite this article:
Chen S, Chu Z, Cao L, et al. Antibacterial mechanism and transcriptomic analysis of a near-infrared triggered upconversion nanoparticles@AgBiS2 for synergetic bacteria-infected therapy. Nano Research, 2022, 15(10): 9298-9308. https://doi.org/10.1007/s12274-022-4815-3
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Abstract Full text Electronic supplementary material About this article

Methicillin-resistant Staphylococcus aureus (MRSA) has become a rising clinical problem as its occurrence has increased due to the overuse and misuse of antibiotics. In this work, upconversion nanoparticles@AgBiS2 core–shell were produced with enhanced photothermal transformation efficiency and ability to produce reactive oxygen species for synergistic photodynamic photothermal and photodynamic antibacterial performance. The nanoparticles exhibit good antibacterial effects in vitro and satisfactory therapeutic performance on healing MRSA-infected wounds in vivo experiments. RNA-sequencing technique has been used to investigate and reveal that photothermal–photodynamic therapy using the nanoparticles can interfere with metabolic processes such as galactose metabolism in MRSA bacteria, destroy the transport system on the surface of MRSA, and affect quorum sensing to hinder the formation of biofilms to achieve effective antibacterial efficacy. It was demonstrated that this work presents an alternative near-infrared photoactive multimodal nanostructure for antibacterial applications.


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Electronic supplementary material
About this article

Antibacterial mechanism and transcriptomic analysis of a near-infrared triggered upconversion nanoparticles@AgBiS2 for synergetic bacteria-infected therapy

Show Author's information Shi Chen1,§, Zhaoyou Chu2,§, Limian Cao1,§, Lingling Xu2( ), Qianqian Jin2, Nian Liu1, Benjin Chen2, Ming Fang1, Wanni Wang2, Haisheng Qian2( ), Min Shao1( )
Department of Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China

§ Shi Chen, Zhaoyou Chu, and Limian Cao contributed equally to this work.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has become a rising clinical problem as its occurrence has increased due to the overuse and misuse of antibiotics. In this work, upconversion nanoparticles@AgBiS2 core–shell were produced with enhanced photothermal transformation efficiency and ability to produce reactive oxygen species for synergistic photodynamic photothermal and photodynamic antibacterial performance. The nanoparticles exhibit good antibacterial effects in vitro and satisfactory therapeutic performance on healing MRSA-infected wounds in vivo experiments. RNA-sequencing technique has been used to investigate and reveal that photothermal–photodynamic therapy using the nanoparticles can interfere with metabolic processes such as galactose metabolism in MRSA bacteria, destroy the transport system on the surface of MRSA, and affect quorum sensing to hinder the formation of biofilms to achieve effective antibacterial efficacy. It was demonstrated that this work presents an alternative near-infrared photoactive multimodal nanostructure for antibacterial applications.

Keywords: photodynamic therapy, photothermal therapy, anti-methicillin-resistant Staphylococcus aureus (MRSA), RNA-sequencing, transcriptomic analysis

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Publication history
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Acknowledgements

Publication history

Received: 15 June 2022
Revised: 22 July 2022
Accepted: 25 July 2022
Published: 26 August 2022
Issue date: October 2022

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 52172276).

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