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Nano Research 2023, 16(1): 599-612 https://doi.org/10.1007/s12274-022-4813-5
Research Article | Issue | Published: 03 September 2022

Near-field electrospun PCL fibers/GelMA hydrogel composite dressing with controlled deferoxamine-release ability and retiform surface for diabetic wound healing

Show Author's Information Huiling Zhong1,2,§,  Jun Huang3,§,  Moucheng Luo2,  Yifei Fang1,2,  Xinchen Zeng4,  Jun Wu1,2( ),  Jianhang Du1,5( )
Medical Research Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, China
School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, China
Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, China
Department of Liver Surgery, The Third People’s Hospital of Shenzhen, Shenzhen 518112, China
NHC Key Laboratory of Assisted Circulation, Sun Yat-sen University, Guangzhou 510080, China

§ Huiling Zhong and Jun Huang contributed equally to this work.

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hydrogel, diabetic wound healing, near field electrospinning, large pore
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Zhong H, Huang J, Luo M, et al. Near-field electrospun PCL fibers/GelMA hydrogel composite dressing with controlled deferoxamine-release ability and retiform surface for diabetic wound healing. Nano Research, 2023, 16(1): 599-612. https://doi.org/10.1007/s12274-022-4813-5
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Abstract Full text Electronic supplementary material About this article

Wound ulceration caused by diabetes is a typical chronic wound wherein healing the local tissue is difficult due to lack of blood vessels and tissue necrosis caused by the long-term accumulation of free radicals. Near-field electrospinning (NFES) is an innovative technology used to produce micro-nano-scaled, controllable sequencing fibers. In this study, we constructed a novel wound dressing based on the NFES polycaprolactone (PCL) fiber network and modified gelatin with methacrylic anhydride (GelMA) hydrogel to promote angiogenesis and the re-epithelialization of diabetic wounds. An angiogenic and antioxidant drug named deferoxamine (DFO) was encapsulated in a GelMA hydrogel to achieve a slow-release effect that is more suitable for chronic wounds. The cell adhesion experiment showed that the cells could attach to the fibers in the dressing group having a network of PCL fibers on the surface and grow along the direction of the fibers, which in turn, effectively regulates cell behavior from the physical structure. Additionally, the large pore size (~ 500 μm) of the network allowed the cells to penetrate the pores and enter the surface of the hydrogel without being blocked out. Besides, the composite dressing had a notable effect on angiogenesis. Furthermore, antioxidation experiments confirmed that the DFO-loaded hydrogel exhibited antioxidant activity. Experimental animal models of diabetes showed that rats treated with the PCL-GelMA-DFO (PGD) hydrogel had faster ability of hemostasis, scab formation, and wound healing. In conclusion, the PGD hydrogel effectively promoted the repair of chronic wounds.


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About this article

Near-field electrospun PCL fibers/GelMA hydrogel composite dressing with controlled deferoxamine-release ability and retiform surface for diabetic wound healing

Show Author's information Huiling Zhong1,2,§, Jun Huang3,§, Moucheng Luo2, Yifei Fang1,2, Xinchen Zeng4, Jun Wu1,2( ), Jianhang Du1,5( )
Medical Research Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, China
School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, China
Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, China
Department of Liver Surgery, The Third People’s Hospital of Shenzhen, Shenzhen 518112, China
NHC Key Laboratory of Assisted Circulation, Sun Yat-sen University, Guangzhou 510080, China

§ Huiling Zhong and Jun Huang contributed equally to this work.

Abstract

Wound ulceration caused by diabetes is a typical chronic wound wherein healing the local tissue is difficult due to lack of blood vessels and tissue necrosis caused by the long-term accumulation of free radicals. Near-field electrospinning (NFES) is an innovative technology used to produce micro-nano-scaled, controllable sequencing fibers. In this study, we constructed a novel wound dressing based on the NFES polycaprolactone (PCL) fiber network and modified gelatin with methacrylic anhydride (GelMA) hydrogel to promote angiogenesis and the re-epithelialization of diabetic wounds. An angiogenic and antioxidant drug named deferoxamine (DFO) was encapsulated in a GelMA hydrogel to achieve a slow-release effect that is more suitable for chronic wounds. The cell adhesion experiment showed that the cells could attach to the fibers in the dressing group having a network of PCL fibers on the surface and grow along the direction of the fibers, which in turn, effectively regulates cell behavior from the physical structure. Additionally, the large pore size (~ 500 μm) of the network allowed the cells to penetrate the pores and enter the surface of the hydrogel without being blocked out. Besides, the composite dressing had a notable effect on angiogenesis. Furthermore, antioxidation experiments confirmed that the DFO-loaded hydrogel exhibited antioxidant activity. Experimental animal models of diabetes showed that rats treated with the PCL-GelMA-DFO (PGD) hydrogel had faster ability of hemostasis, scab formation, and wound healing. In conclusion, the PGD hydrogel effectively promoted the repair of chronic wounds.

Keywords: hydrogel, diabetic wound healing, near field electrospinning, large pore

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Publication history
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Acknowledgements

Publication history

Received: 14 June 2022
Revised: 19 July 2022
Accepted: 25 July 2022
Published: 03 September 2022
Issue date: January 2023

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

We sincerely acknowledge the funding from the Science and Technology Planning Project of Shenzhen Municipality (Nos. JCYJ20180306174831458 and JCYJ20190807155801657), the National Natural Science Foundation of China (Nos. 51973243 and 52173150), Guangdong Innovative and Entrepreneurial Research Team Program (No. 2016ZT06S029), and Key international (regional) cooperative research projects of the National Natural Science Foundation of China (No. 5181001045). The International Science and technology cooperation project of Shenzhen Municipality (No. GJHZ20200731095008026), Public welfare project of Futian District Health Bureau (No. FTWS2021001).

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