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Nano Research 2022, 15(5): 4285-4293 https://doi.org/10.1007/s12274-022-4085-0
Research Article | Issue | Published: 29 January 2022

Indocyanine green assembled free oxygen-nanobubbles towards enhanced near-infrared induced photodynamic therapy

Show Author's Information Li Yang1,  Bin Huang1,2( ),  Shiqi Hu3,  Yuan An1,  Jingyi Sheng1,  Yan Li1,  Yuxin Wang3,  Ning Gu1( )
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
College of Life Sciences and Chemistry, Jiangsu Second Normal University, Nanjing 210013, China
Nanjing Stomatology Hospital, Nanjing 210008, China
Keywords:
photodynamic therapy, free oxygen-nanobubbles, aqueous solution stability, singlet oxygen quantum yield, indocyanine green
Topics:
Biomaterials
Cite this article:
Yang L, Huang B, Hu S, et al. Indocyanine green assembled free oxygen-nanobubbles towards enhanced near-infrared induced photodynamic therapy. Nano Research, 2022, 15(5): 4285-4293. https://doi.org/10.1007/s12274-022-4085-0
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Abstract Full text Electronic supplementary material About this article

Photodynamic therapy (PDT) has shown a promising capability for cancer treatment with minimal side effects. Indocyanine green (ICG), the only clinically approved near-infrared (NIR) fluorophore, has been used as a photosensitizer for PDT in clinical application. However, the main obstacle of directly utilizing ICG in the clinic lies in its low singlet oxygen (1O2) quantum yield (QY) and instability in aqueous solution. To improve the PDT efficacy of ICG, free ICG molecules were assembled with free oxygen nanobubbles (NBs-O2) to fabricate ICG-NBs-O2 by hydrophilic–hydrophobe interactions on the gas–liquid interface. Interestingly, 1O2 QY of ICG-NBs-O2 solution was significantly increased to 1.6%, which was estimated to be 8 times as high as that of free ICG solution. Meanwhile, ICG-NBs-O2 exhibited better aqueous solution stability compared with free ICG. Furthermore, through establishing tumor models in nude mice, the therapeutic efficacy of ICG-NBs-O2 was also assessed in the PDT treatment of oral cancer. The tumor volume in ICG-NBs-O2 treated group on day 14 decreased to 0.56 of the initial tumor size on day 1, while the tumor volume in free ICG treated group increased to 2.4 times. The results demonstrated that ICG-NBs-O2 showed excellent tumor ablation in vivo. Therefore, this facile method provided an effective strategy for enhanced PDT treatment of ICG and showed great potential in clinical application.


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About this article

Indocyanine green assembled free oxygen-nanobubbles towards enhanced near-infrared induced photodynamic therapy

Show Author's information Li Yang1, Bin Huang1,2( ), Shiqi Hu3, Yuan An1, Jingyi Sheng1, Yan Li1, Yuxin Wang3, Ning Gu1( )
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
College of Life Sciences and Chemistry, Jiangsu Second Normal University, Nanjing 210013, China
Nanjing Stomatology Hospital, Nanjing 210008, China

Abstract

Photodynamic therapy (PDT) has shown a promising capability for cancer treatment with minimal side effects. Indocyanine green (ICG), the only clinically approved near-infrared (NIR) fluorophore, has been used as a photosensitizer for PDT in clinical application. However, the main obstacle of directly utilizing ICG in the clinic lies in its low singlet oxygen (1O2) quantum yield (QY) and instability in aqueous solution. To improve the PDT efficacy of ICG, free ICG molecules were assembled with free oxygen nanobubbles (NBs-O2) to fabricate ICG-NBs-O2 by hydrophilic–hydrophobe interactions on the gas–liquid interface. Interestingly, 1O2 QY of ICG-NBs-O2 solution was significantly increased to 1.6%, which was estimated to be 8 times as high as that of free ICG solution. Meanwhile, ICG-NBs-O2 exhibited better aqueous solution stability compared with free ICG. Furthermore, through establishing tumor models in nude mice, the therapeutic efficacy of ICG-NBs-O2 was also assessed in the PDT treatment of oral cancer. The tumor volume in ICG-NBs-O2 treated group on day 14 decreased to 0.56 of the initial tumor size on day 1, while the tumor volume in free ICG treated group increased to 2.4 times. The results demonstrated that ICG-NBs-O2 showed excellent tumor ablation in vivo. Therefore, this facile method provided an effective strategy for enhanced PDT treatment of ICG and showed great potential in clinical application.

Keywords: photodynamic therapy, free oxygen-nanobubbles, aqueous solution stability, singlet oxygen quantum yield, indocyanine green

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Publication history
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Acknowledgements

Publication history

Received: 16 October 2021
Revised: 15 December 2021
Accepted: 19 December 2021
Published: 29 January 2022
Issue date: May 2022

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

This investigation was financially funded by the projects of the National Key Research and Development Program of China (No. 2017YFA0104302), the National Natural Science Foundation of China (Nos. 51832001, 61821002, and 81971750), and the Jiangsu Planned Projects for Postdoctoral Research Funds (No. 2021K601C).

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