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Nano Research 2022, 15(5): 4569-4574 https://doi.org/10.1007/s12274-022-4078-z
Research Article | Issue | Published: 08 February 2022

Fine-tuned mesoporous covalent organic frameworks for highly efficient low molecular-weight proteins separation

Show Author's Information Tienan Wang§,  Irfan Azhar§,  Yuting Yang,  Yao Lu,  Yuyang Tian,  Nan Gao,  Fengchao Cui( ),  Li Yang( ),  Xiaofei Jing( ),  Guangshan Zhu
Department of Chemistry, Northeast Normal University, Changchun 130024, China

§ Tienan Wang and Irfan Azhar contributed equally to this work.

Keywords:
separation, mesoporous materials, covalent organic frameworks, pore regulation, low molecular-weight protein
Topics:
Nano biology
Cite this article:
Wang T, Azhar I, Yang Y, et al. Fine-tuned mesoporous covalent organic frameworks for highly efficient low molecular-weight proteins separation. Nano Research, 2022, 15(5): 4569-4574. https://doi.org/10.1007/s12274-022-4078-z
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Abstract Full text Electronic supplementary material About this article

Covalent organic frameworks (COFs) are prominent porous materials for molecules separation due to their desirable structures. However, very few COFs are reported for the separation of macromolecules such as low molecular-weight (MW) proteins. Here, two stable mesoporous COFs (Azo-COF and Tp-COF) with highly crystallized frameworks are synthesized, and their pore sizes are slightly-regulated via elaborate selection of pyrene knots and amino linkages. Benefiting from the pore size difference less than 4 Å, the tandem utilization of these two COFs exhibits efficiently size-selective separation ability towards low MW proteins cytochrome c and myoglobin with small MW difference of 2 kDa, in which protein adsorption possibilities are verified by computational calculations together with confocal laser scanning microscopy (CLSM). Furthermore, a simple COF-based separation device is designed and prepared to achieve effective and low-consumption proteins separation. This work has offered an optimized synthetic strategy for fine-tuned mesoporous COFs and expanded their applications on macromolecules separation.


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About this article

Fine-tuned mesoporous covalent organic frameworks for highly efficient low molecular-weight proteins separation

Show Author's information Tienan Wang§, Irfan Azhar§, Yuting Yang, Yao Lu, Yuyang Tian, Nan Gao, Fengchao Cui( ), Li Yang( ), Xiaofei Jing( ), Guangshan Zhu
Department of Chemistry, Northeast Normal University, Changchun 130024, China

§ Tienan Wang and Irfan Azhar contributed equally to this work.

Abstract

Covalent organic frameworks (COFs) are prominent porous materials for molecules separation due to their desirable structures. However, very few COFs are reported for the separation of macromolecules such as low molecular-weight (MW) proteins. Here, two stable mesoporous COFs (Azo-COF and Tp-COF) with highly crystallized frameworks are synthesized, and their pore sizes are slightly-regulated via elaborate selection of pyrene knots and amino linkages. Benefiting from the pore size difference less than 4 Å, the tandem utilization of these two COFs exhibits efficiently size-selective separation ability towards low MW proteins cytochrome c and myoglobin with small MW difference of 2 kDa, in which protein adsorption possibilities are verified by computational calculations together with confocal laser scanning microscopy (CLSM). Furthermore, a simple COF-based separation device is designed and prepared to achieve effective and low-consumption proteins separation. This work has offered an optimized synthetic strategy for fine-tuned mesoporous COFs and expanded their applications on macromolecules separation.

Keywords: separation, mesoporous materials, covalent organic frameworks, pore regulation, low molecular-weight protein

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Publication history
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Acknowledgements

Publication history

Received: 13 October 2021
Revised: 27 November 2021
Accepted: 19 December 2021
Published: 08 February 2022
Issue date: May 2022

Copyright

© Tsinghua University Press 2022

Acknowledgements

Acknowledgements

The authors are grateful for the financial support from the Fundamental Research Funds for the Central Universities (No. 2412019FZ008), the National Natural Science Foundation of China (Nos. 21503038 and 22074014), and the “111” project (No. B18012).

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