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Nano Research 2022, 15(3): 2479-2488 https://doi.org/10.1007/s12274-021-3734-z
Research Article | Issue | Published: 26 August 2021

Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B6 in energy drinks and dietary supplements

Show Author's Information Lu Zeng1,2,  Xinxin Xu1,2,  Shanshan Song1,2,  Liguang Xu1,2,  Liqiang Liu1,2,  Jing Xiao3( ),  Chuanlai Xu1,2,  Hua Kuang1,2( )
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100022, China
Keywords:
vitamin B6, monoclonal antibody, immunochromatographic strip test
Topics:
Nanosensors
Cite this article:
Zeng L, Xu X, Song S, et al. Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B6 in energy drinks and dietary supplements. Nano Research, 2022, 15(3): 2479-2488. https://doi.org/10.1007/s12274-021-3734-z
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Abstract Full text About this article

We designed and synthesized novel haptens to produce monoclonal antibodies (mAb) against vitamin B6 (VB6). A group-specific mAb (2F9) that recognized pyridoxine (PN), pyridoxamine (PM), and pyridoxal (PL) was prepared using a homologous strategy with 50% maximal inhibitory concentration (IC50) values of 106.60, 250.57, and 400.11 ng/mL, respectively. Based on this, a gold nanoparticles (AuNPs)-based immunochromatographic strip (ICS) test was established for the detection of VB6 in energy drinks and B-vitamin complex tablets. The developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min, and displayed the visual limit of detection (vLOD) values of 250, 500, and 1,000 ng/mL for PN, PM, and PL, respectively. For quantitative analysis, the results obtained by strip reader, with calculated LOD values for PN, PM, and PL were 14.10, 55.58, and 56.25 ng/mL, respectively. Commercial energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid chromatography. Overall, the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements.


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About this article

Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B6 in energy drinks and dietary supplements

Show Author's information Lu Zeng1,2, Xinxin Xu1,2, Shanshan Song1,2, Liguang Xu1,2, Liqiang Liu1,2, Jing Xiao3( ), Chuanlai Xu1,2, Hua Kuang1,2( )
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100022, China

Abstract

We designed and synthesized novel haptens to produce monoclonal antibodies (mAb) against vitamin B6 (VB6). A group-specific mAb (2F9) that recognized pyridoxine (PN), pyridoxamine (PM), and pyridoxal (PL) was prepared using a homologous strategy with 50% maximal inhibitory concentration (IC50) values of 106.60, 250.57, and 400.11 ng/mL, respectively. Based on this, a gold nanoparticles (AuNPs)-based immunochromatographic strip (ICS) test was established for the detection of VB6 in energy drinks and B-vitamin complex tablets. The developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min, and displayed the visual limit of detection (vLOD) values of 250, 500, and 1,000 ng/mL for PN, PM, and PL, respectively. For quantitative analysis, the results obtained by strip reader, with calculated LOD values for PN, PM, and PL were 14.10, 55.58, and 56.25 ng/mL, respectively. Commercial energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid chromatography. Overall, the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements.

Keywords: vitamin B6, monoclonal antibody, immunochromatographic strip test

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Publication history
Copyright
Acknowledgements

Publication history

Received: 19 May 2021
Revised: 03 July 2021
Accepted: 06 July 2021
Published: 26 August 2021
Issue date: March 2022

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021

Acknowledgements

Acknowledgements

This work was funded by the Key Programs from MOST (No. 2019YFC1605004).

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