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Nano Research 2022, 15(2): 1623-1635 https://doi.org/10.1007/s12274-021-3711-6
Research Article | Issue | Published: 12 August 2021

Critical adhesion areas of cells on micro-nanopatterns

Show Author's Information Shuang Zheng1,  Qiong Liu1,2,  Junhao He1,  Xinlei Wang1,  Kai Ye1,  Xuan Wang1,  Ce Yan1,  Peng Liu1,3,  Jiandong Ding1( )
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science Fudan UniversityShanghai 200438 China
Navy Characteristic Medical Center, the Second Military Medical UniversityShanghai 200433 China
College of Bioengineering Chongqing UniversityChongqing 400044 China
Keywords:
poly(ethylene glycol), biomaterial, cell adhesion, surface patterning, arginine–glycine–aspartate (RGD) nanospacing, critical adhesion area
Topics:
Biomaterials
Cite this article:
Zheng S, Liu Q, He J, et al. Critical adhesion areas of cells on micro-nanopatterns. Nano Research, 2022, 15(2): 1623-1635. https://doi.org/10.1007/s12274-021-3711-6
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Abstract Full text Electronic supplementary material About this article

Cell adhesion to extracellular matrices (ECM) is critical to physiological and pathological processes as well as biomedical and biotechnological applications. It has been known that a cell can adhere on an adhesive microisland only over a critical size. But no publication has concerned critical adhesion areas of cells on microislands with nanoarray decoration. Herein, we fabricated a series of micro-nanopatterns with different microisland sizes and arginine–glycine–aspartate (RGD) nanospacings on a nonfouling poly(ethylene glycol) background. Besides reproducing that nanospacing of RGD, a ligand of its receptor integrin (a membrane protein), significantly influences specific cell adhesion on bioactive nanoarrays, we confirmed that the concept of critical adhesion area originally suggested in studies of cells on micropatterns was justified also on the micro-nanopatterns, yet the latter exhibited more characteristic behaviors of cell adhesion. We found increased critical adhesion areas of human mesenchymal stem cells (hMSCs) on nanoarrayed microislands with increased RGD nanospacings. However, the numbers of nanodots with respect to the critical adhesion areas were not a constant. A unified interpretation was then put forward after combining nonspecific background adhesion and specific cell adhesion. We further carried out the asymptotic analysis of a series of micro-nanopatterned surfaces to obtain the effective RGD nanospacing on unpatterned free surfaces with densely grafted RGD, which could be estimated nonzero but has never been revealed previously without the assistance of the micro-nanopatterning techniques and the corresponding analysis.


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About this article

Critical adhesion areas of cells on micro-nanopatterns

Show Author's information Shuang Zheng1, Qiong Liu1,2, Junhao He1, Xinlei Wang1, Kai Ye1, Xuan Wang1, Ce Yan1, Peng Liu1,3, Jiandong Ding1( )
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science Fudan UniversityShanghai 200438 China
Navy Characteristic Medical Center, the Second Military Medical UniversityShanghai 200433 China
College of Bioengineering Chongqing UniversityChongqing 400044 China

Abstract

Cell adhesion to extracellular matrices (ECM) is critical to physiological and pathological processes as well as biomedical and biotechnological applications. It has been known that a cell can adhere on an adhesive microisland only over a critical size. But no publication has concerned critical adhesion areas of cells on microislands with nanoarray decoration. Herein, we fabricated a series of micro-nanopatterns with different microisland sizes and arginine–glycine–aspartate (RGD) nanospacings on a nonfouling poly(ethylene glycol) background. Besides reproducing that nanospacing of RGD, a ligand of its receptor integrin (a membrane protein), significantly influences specific cell adhesion on bioactive nanoarrays, we confirmed that the concept of critical adhesion area originally suggested in studies of cells on micropatterns was justified also on the micro-nanopatterns, yet the latter exhibited more characteristic behaviors of cell adhesion. We found increased critical adhesion areas of human mesenchymal stem cells (hMSCs) on nanoarrayed microislands with increased RGD nanospacings. However, the numbers of nanodots with respect to the critical adhesion areas were not a constant. A unified interpretation was then put forward after combining nonspecific background adhesion and specific cell adhesion. We further carried out the asymptotic analysis of a series of micro-nanopatterned surfaces to obtain the effective RGD nanospacing on unpatterned free surfaces with densely grafted RGD, which could be estimated nonzero but has never been revealed previously without the assistance of the micro-nanopatterning techniques and the corresponding analysis.

Keywords: poly(ethylene glycol), biomaterial, cell adhesion, surface patterning, arginine–glycine–aspartate (RGD) nanospacing, critical adhesion area

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Publication history
Copyright
Acknowledgements

Publication history

Received: 29 April 2021
Revised: 19 June 2021
Accepted: 24 June 2021
Published: 12 August 2021
Issue date: February 2022

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021

Acknowledgements

Acknowledgements

This work was financially supported by the National Key R & D Program of China (No. 2016YFC1100300) and the National Natural Science Foundation of China (Nos. 21961160721 and 21704018).

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