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Nano Research 2021, 14(11): 4288-4293 https://doi.org/10.1007/s12274-021-3689-0
Research Article | Issue | Published: 10 July 2021

Spatiotemporal sectioning of two-photon fluorescence ellipsoid with a CsPbBr3 nanosheet

Show Author's Information Yizhi Zhu§,  Jinping Chen§,  Qiannan Cui( ),  Heng Guo,  Zhuxin Li,  Zengliang Shi,  Chunxiang Xu( )
State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China

§ Yizhi Zhu and Jinping Chen contributed equally to this work.

Keywords:
photon recycling, spatiotemporal sectioning, two-photon fluorescence ellipsoid, CsPbBr3 nanosheet, photocarrier trapping
Topics:
Two photon processes
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Zhu Y, Chen J, Cui Q, et al. Spatiotemporal sectioning of two-photon fluorescence ellipsoid with a CsPbBr3 nanosheet. Nano Research, 2021, 14(11): 4288-4293. https://doi.org/10.1007/s12274-021-3689-0
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Abstract Full text Electronic supplementary material About this article

Two-photon fluorescence (TPF) ellipsoid formed by a focused femtosecond laser into luminescent media serves as a fundamental pixel for TPF spatiotemporal imaging. Visualizing spatiotemporal evolution of the TPF ellipsoid itself in a selected luminescent medium is important for correctly reconstructing and interpreting spatiotemporal information of imaged targets. Here, we report a new spatiotemporal sectioning technique with a luminescent CsPbBr3 nanosheet and visualize the spatiotemporal evolution of TPF ellipsoid along the axial direction. Time-resolved axial lengths of TPF ellipsoids turn out to broaden nonlinearly with a turning point at about 600 ps. By comparison experiments, observed phenomena are attributed to photocarrier trapping and TPF photon recycling processes within CsPbBr3 nanosheets. The spatiotemporal sectioning technique is expected to be widely applicable, which will ignite a plethora of investigations and applications utilizing TPF ellipsoid.


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Electronic supplementary material
About this article

Spatiotemporal sectioning of two-photon fluorescence ellipsoid with a CsPbBr3 nanosheet

Show Author's information Yizhi Zhu§, Jinping Chen§, Qiannan Cui( ), Heng Guo, Zhuxin Li, Zengliang Shi, Chunxiang Xu( )
State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China

§ Yizhi Zhu and Jinping Chen contributed equally to this work.

Abstract

Two-photon fluorescence (TPF) ellipsoid formed by a focused femtosecond laser into luminescent media serves as a fundamental pixel for TPF spatiotemporal imaging. Visualizing spatiotemporal evolution of the TPF ellipsoid itself in a selected luminescent medium is important for correctly reconstructing and interpreting spatiotemporal information of imaged targets. Here, we report a new spatiotemporal sectioning technique with a luminescent CsPbBr3 nanosheet and visualize the spatiotemporal evolution of TPF ellipsoid along the axial direction. Time-resolved axial lengths of TPF ellipsoids turn out to broaden nonlinearly with a turning point at about 600 ps. By comparison experiments, observed phenomena are attributed to photocarrier trapping and TPF photon recycling processes within CsPbBr3 nanosheets. The spatiotemporal sectioning technique is expected to be widely applicable, which will ignite a plethora of investigations and applications utilizing TPF ellipsoid.

Keywords: photon recycling, spatiotemporal sectioning, two-photon fluorescence ellipsoid, CsPbBr3 nanosheet, photocarrier trapping

References(52)

1

Denk, W.; Strickler, J. H.; Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 1990, 248, 73–76.
DOI Google Scholar
2

Helmchen, F.; Denk, W. Deep tissue two-photon microscopy. Nat. Methods 2005, 2, 932–940.
DOI Google Scholar
3

Young, M. D.; Field, J. J.; Sheetz, K. E.; Bartels, R. A.; Squier, J. A pragmatic guide to multiphoton microscope design. Adv. Opt. Photonics 2015, 7, 276–378.
DOI Google Scholar
4

Ntziachristos, V. Going deeper than microscopy: The optical imaging frontier in biology. Nat. Methods 2010, 7, 603–614.
DOI Google Scholar
5

Qi, J.; Sun, C. W.; Li, D. Y.; Zhang, H. Q.; Yu, W. B.; Zebibula, A.; Lam, J. W. Y.; Xi, W.; Zhu, L.; Cai, F. H. et al. Aggregation-induced emission luminogen with near-infrared-Ⅱ excitation and near-infrared-Ⅰ emission for ultradeep intravital two-photon microscopy. ACS Nano 2018, 12, 7936–7945.
DOI Google Scholar
6

Beaulieu, D. R.; Davison, I. G.; Kiliç, K.; Bifano, T. G.; Mertz, J. Simultaneous multiplane imaging with reverberation two-photon microscopy. Nat. Methods 2020, 17, 283–286.
DOI Google Scholar
7

Bastiaens, P. I. H.; Squire, A. Fluorescence lifetime imaging microscopy: Spatial resolution of biochemical processes in the cell. Trends Cell Biol. 1999, 9, 48–52.
DOI Google Scholar
8

Berndt, M.; Lorenz, M.; Enderlein, J.; Diez, S. Axial nanometer distances measured by fluorescence lifetime imaging microscopy. Nano Lett. 2010, 10, 1497–1500.
DOI Google Scholar
9

Orte, A.; Alvarez-Pez, J. M.; Ruedas-Rama, M. J. Fluorescence lifetime imaging microscopy for the detection of intracellular pH with quantum dot nanosensors. ACS Nano 2013, 7, 6387–6395.
DOI Google Scholar
10

Zheng, K. Y.; Jensen, T. P.; Rusakov, D. A. Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging. Nat. Protoc. 2018, 13, 581–597.
DOI Google Scholar
11

Camborde, L.; Jauneau, A.; Brière, C.; Deslandes, L.; Dumas, B.; Gaulin, E. Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy. Nat. Protoc. 2017, 12, 1933–1950.
DOI Google Scholar
12

Niehörster, T.; Löschberger, A.; Gregor, I.; Krämer, B.; Rahn, H. J.; Patting, M.; Koberling, F.; Enderlein, J.; Sauer, M. Multi-target spectrally resolved fluorescence lifetime imaging microscopy. Nat. Methods 2016, 13, 257–262.
DOI Google Scholar
13

Karpf, S.; Riche, C. T.; Di Carlo, D.; Goel, A.; Zeiger, W. A.; Suresh, A.; Portera-Cailliau, C.; Jalali, B. Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates. Nat. Commun. 2020, 11, 2062.
DOI Google Scholar
14

Rumi, M.; Perry, J. W. Two-photon absorption: An overview of measurements and principles. Adv. Opt. Photonics 2010, 2, 451–518.
DOI Google Scholar
15

Cui, Q. N.; Li, Y. Y.; Chang, J. H.; Zhao, H.; Xu, C. X. Temporally resolving synchronous degenerate and nondegenerate two-photon absorption in 2D semiconducting monolayers. Laser Photonics Rev. 2019, 13, 1800225.
DOI Google Scholar
16

Saouma, F. O.; Stoumpos, C. C.; Kanatzidis, M. G.; Kim, Y. S.; Jang, J. I. Multiphoton absorption order of CsPbBr3 As determined by wavelength-dependent nonlinear optical spectroscopy. J. Phys. Chem. Lett. 2017, 8, 4912–4917.
DOI Google Scholar
17

Lu, W. G.; Chen, C.; Han, D. B.; Yao, L. H.; Han, J. B.; Zhong, H. Z.; Wang, Y. T. Nonlinear optical properties of colloidal CH3NH3PbBr3 and CsPbBr3 quantum dots: a comparison study using Z-scan technique. Adv. Opt. Mater. 2016, 4, 1732–1737.
DOI Google Scholar
18

Xu, Y. Q.; Chen, Q.; Zhang, C. F.; Wang, R.; Wu, H.; Zhang, X. Y.; Xing, G. C.; Yu, W. W.; Wang, X. Y.; Zhang, Y. et al. Two-photon- pumped perovskite semiconductor nanocrystal lasers. J. Am. Chem. Soc. 2016, 138, 3761–3768.
DOI Google Scholar
19

Wang, S. L.; Yang, F.; Zhu, J. R.; Cao, Q. X.; Zhong, Y. G.; Wang, A. C.; Du, W. N.; Liu, X. F. Growth of metal halide perovskite materials. Sci. China Mater. 2020, 63, 1438–1463.
DOI Google Scholar
20

Zhong, Y. G.; Liao, K.; Du, W. N.; Zhu, J. R.; Shang, Q. Y.; Zhou, F.; Wu, X. X.; Sui, X. Y.; Shi, J. W.; Yue, S. et al. Large-scale thin CsPbBr3 single-crystal film grown on sapphire via chemical vapor deposition: Toward laser array application. ACS Nano 2020, 14, 15605–15615.
DOI Google Scholar
21

Zhang, S.; Shang, Q. Y.; Du, W. N.; Shi, J.; Wu, Z. Y.; Mi, Y.; Chen, J.; Liu, F. J.; Li, Y. Z.; Liu, M. et al. Strong exciton–photon coupling in hybrid inorganic–organic perovskite micro/nanowires. Adv. Opt. Mater. 2018, 6, 1701032.
DOI Google Scholar
22

Jeong, M. Y.; Choi, I. W.; Go, E. M.; Cho, Y.; Kim, M.; Lee, B.; Jeong, S.; Jo, Y.; Choi, H. W.; Lee, J. et al. Stable perovskite solar cells with efficiency exceeding 24.8% and 0.3-V voltage loss. Science 2020, 369, 1615–1620.
DOI Google Scholar
23

Jia, Y. F.; Kerner, R. A.; Grede, A. J.; Rand, B. P.; Giebink, N. C. Continuous-wave lasing in an organic–inorganic lead halide perovskite semiconductor. Nat. Photonics 2017, 11, 784–788.
DOI Google Scholar
24

Shang, Q. Y.; Li, M. L.; Zhao, L. Y.; Chen, D. W.; Zhang, S.; Chen, S. L.; Gao, P.; Shen, C.; Xing, J.; Xing, G. C. et al. Role of the exciton–polariton in a continuous-wave optically pumped CsPbBr3 perovskite laser. Nano Lett. 2020, 20, 6636–6643.
DOI Google Scholar
25

Lin, K. B.; Xing, J.; Quan, L. N.; De Arquer, F. P. G.; Gong, X. W.; Lu, J. X.; Xie, L. Q.; Zhao, W. J.; Zhang, D.; Yan, C. Z. et al. Perovskite light-emitting diodes with external quantum efficiency exceeding 20 percent. Nature 2018, 562, 245–248.
DOI Google Scholar
26

Rubart, M. Two-photon microscopy of cells and tissue. Circ. Res. 2004, 95, 1154–1166.
DOI Google Scholar
27

Polyzos, I.; Tsigaridas, G.; Fakis, M.; Parthenios, J.; Fragos, A.; Giannetas, V.; Persephonis, P.; Mikroyannidis, J. Examination of the spatial distribution of dyes and polymers in thin films by two- photon microscopy. Monatsh. Chem. 2001, 132, 169–175.
DOI Google Scholar
28

Soeller, C.; Cannell, M. B. Two-photon microscopy: Imaging in scattering samples and three-dimensionally resolved flash photolysis. Microsc. Res. Techniq. 1999, 47, 182–195.
DOI
29

Mannino, G.; Deretzis, I.; Smecca, E.; La Magna, A.; Alberti, A.; Ceratti, D.; Cahen, D. Temperature-dependent optical band gap in CsPbBr3, MAPbBr3, and FAPbBr3 single crystals. J. Phys. Chem. Lett. 2020, 11, 2490–2496.
DOI Google Scholar
30

Tao, S. X.; Schmidt, I.; Brocks, G.; Jiang, J. K.; Tranca, I.; Meerholz, K.; Olthof, S. Absolute energy level positions in tin-and lead-based halide perovskites. Nat. Commun. 2019, 10, 2560.
DOI Google Scholar
31

Walters, G.; Sutherland, B. R.; Hoogland, S.; Shi, D.; Comin, R.; Sellan, D. P.; Bakr, O. M.; Sargent, E. H. Two-photon absorption in organometallic bromide perovskites. ACS Nano 2015, 9, 9340–9346.
DOI Google Scholar
32

Wei, T. C.; Mokkapati, S.; Li, T. Y.; Lin, C. H.; Lin, G. R.; Jagadish, C.; He, J. H. Nonlinear absorption applications of CH3NH3PbBr3 perovskite crystals. Adv. Funct. Mater. 2018, 28, 1707175.
DOI Google Scholar
33

Wang, Y.; Li, X. M.; Zhao, X.; Xiao, L.; Zeng, H. B.; Sun, H. D. Nonlinear absorption and low-threshold multiphoton pumped stimulated emission from all-inorganic perovskite nanocrystals. Nano Lett. 2016, 16, 448–453.
DOI Google Scholar
34

Dai, J.; Xu, C. X.; Sun, L. X.; Chen, Z. H.; Guo, J. Y.; Li, Z. H. Multiphoton absorption-induced optical whispering-gallery modes in ZnO microcavities at room temperature. J. Phys. D Appl. Phys. 2010, 44, 025404.
DOI Google Scholar
35

Sun, H. B.; Maeda, M.; Takada, K.; Chon, J. W. M.; Gu, M.; Kawata, S. Experimental investigation of single voxels for laser nanofabrication via two-photon photopolymerization. Appl. Phys. Lett. 2003, 83, 819–821.
DOI Google Scholar
36

Sun, H. B.; Tanaka, T.; Kawata, S. Three-dimensional focal spots related to two-photon excitation. Appl. Phys. Lett. 2002, 80, 3673– 3675.
DOI Google Scholar
37

Sun, H. B.; Takada, K.; Kim, M. S.; Lee, K. S; Kawata, S. Scaling laws of voxels in two-photon photopolymerization nanofabrication. Appl. Phys. Lett. 2003, 83, 1104–1106.
DOI Google Scholar
38
Kubitscheck, U. Principles of light microscopy. In Fluorescence Microscopy: From Principles to Biological Applications. Masters, B. R., Ed.; Wiley: Bonn, 2013; pp 33–95.https://doi.org/10.1002/9783527671595
DOI
39

Eaton, S. W.; Lai, M.; Gibson, N. A.; Wong, A. B.; Dou, L.; Ma, J.; Wang, L. W.; Leone, S. R.; Yang, P. D. Lasing in robust cesium lead halide perovskite nanowires. Proc. Natl. Acad. Sci. USA 2016, 113, 1993–1998.
DOI Google Scholar
40

Dong, Q. F.; Fang Y. J.; Shao Y. C.; Mulligan, P.; Qiu, J.; Cao L.; Huang J. S. Electron-hole diffusion lengths > 175 μm in solution- grown CH3NH3PbI3 single crystals. Science 2015, 347, 967–970.
DOI Google Scholar
41

Cui, Q. N.; Ceballos, F.; Kumar, N.; Zhao, H. Transient absorption microscopy of monolayer and bulk WSe2. ACS Nano 2014, 8, 2970–2976.
DOI Google Scholar
42

Lu, N. D.; Wang, J. W.; Geng, D.; Li, L.; Liu, M. Understanding the transport mechanism of organic-inorganic perovskite solar cells: the effect of exciton or free-charge on diffusion length. Org. Electron. 2019, 66, 163–168.
DOI Google Scholar
43

Crothers, T. W.; Milot, R. L.; Patel, J. B.; Parrott, E. S.; Schlipf, J.; Müller-Buschbaum, P.; Johnston, M. B.; Herz, L. M. Photon reabsorption masks intrinsic bimolecular charge-carrier recombination in CH3NH3PbI3 perovskite. Nano Lett. 2017, 17, 5782–5789.
DOI Google Scholar
44

Motti, S. G.; Crothers, T.; Yang, R.; Cao, Y.; Li, R. Z.; Johnston, M. B.; Wang, J. P.; Herz, L. M. Heterogeneous photon recycling and charge diffusion enhance charge transport in quasi-2D lead-halide perovskite films. Nano Lett. 2019, 19, 3953–3960.
DOI Google Scholar
45

Diab, H.; Arnold, C.; Lédée, F.; Trippé-Allard, G.; Delport, G.; Vilar, C.; Bretenaker, F.; Barjon, J.; Lauret, J. S; Deleporte, E. et al. Impact of reabsorption on the emission spectra and recombination dynamics of hybrid perovskite single crystals. J. Phys. Chem. Lett. 2017, 8, 2977–2983.
DOI Google Scholar
46

Zhu, Y. Z.; Cui, Q. N.; Chen, J. P.; Chen, F.; Shi, Z. L.; Zhao, X. W.; Xu, C. X. Inhomogeneous trap-state-mediated ultrafast photocarrier dynamics in CsPbBr3 microplates. ACS Appl. Mater. Interfaces 2021, 13, 6820–6829.
DOI Google Scholar
47

Dar, M. I.; Jacopin, G.; Meloni, S.; Mattoni, A.; Arora, N.; Boziki, A.; Zakeeruddin, S. M.; Rothlisberger, U.; Grätzel, M. Origin of unusual bandgap shift and dual emission in organic-inorganic lead halide perovskites. Sci. Adv. 2016, 2, e1601156.
DOI Google Scholar
48

He, M.; Jiang, Y.; Liu, Q. B.; Luo, Z.; Ouyang, C. X.; Wang, X. X.; Zheng, W. H.; Braun, K.; Meixner, A. J.; Gao, T. G. et al. Revealing excitonic and electron-hole plasma states in stimulated emission of single CsPbBr3 nanowires at room temperature. Phys. Rev. Appl. 2020, 13, 044072.
DOI Google Scholar
49

Socie, E.; Vale, B. R. C.; Burgos-Caminal, A.; Moser, J. E. Direct observation of shallow trap states in thermal equilibrium with band-edge excitons in strongly confined CsPbBr3 perovskite nanoplatelets. Adv. Opt. Mater. 2021, 9, 2001308.
DOI Google Scholar
50

Godin, R.; Wang, Y.; Zwijnenburg, M. A.; Tang, J.; Durrant, J. R. Time-resolved spectroscopic investigation of charge trapping in carbon nitrides photocatalysts for hydrogen generation. J. Am. Chem. Soc. 2017, 139, 5216–5224.
DOI Google Scholar
51

Gan, Z. X.; Wen, X. M.; Zhou, C. H.; Chen, W. J.; Zheng, F.; Yang, S.; Davis, J. A.; Tapping, P. C.; Kee, T. W.; Zhang, H. et al. Transient energy reservoir in 2D perovskites. Adv. Opt. Mater. 2019, 7, 1900971.
DOI Google Scholar
52

Zhao, C. Y.; Tian, W. M.; Liu, J. X.; Sun, Q.; Luo, J. J.; Yuan, H.; Gai, B. D.; Tang, J.; Guo, J. W.; Jin, S. Y. Stable two-photon pumped amplified spontaneous emission from millimeter-sized CsPbBr3 single crystals. J. Phys. Chem. Lett. 2019, 10, 2357–2362.
DOI Google Scholar
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Publication history
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Acknowledgements

Publication history

Received: 14 April 2021
Revised: 26 May 2021
Accepted: 16 June 2021
Published: 10 July 2021
Issue date: November 2021

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021

Acknowledgements

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 11734005, 61704024, 61821002, and 62075041), Natural Science Foundation of Jiangsu Province (No. BK20170696), the National Key Research and Development Program of China (Nos. 2017YFA0700500 and 2018YFA0209101) and Fundamental Research Funds for the Central Universities (No. 2242021K10009). Q. N. C. gratefully acknowledges the support of Southeast University through Zhishan Young Scholar Fund. The authors thank Dr. Haibo Ding for insightful discussion.

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