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Nano Research 2022, 15(2): 773-786 https://doi.org/10.1007/s12274-021-3573-y
Review Article | Issue | Published: 22 June 2021

FluidFM for single-cell biophysics

Show Author's Information Mi Li1,2,3( ),  Lianqing Liu2,3,  Tomaso Zambelli1
Laboratory of Biosensors and BioelectronicsInstitute for Biomedical Engineering, ETH ZürichZürich8092Switzerland
State Key Laboratory of Robotics, Shenyang Institute of AutomationChinese Academy of SciencesShenyang110016China
Institutes for Robotics and Intelligent ManufacturingChinese Academy of SciencesShenyang110169China
Keywords:
atomic force microscopy, fluidic force microscopy, single-cell manipulation, single-cell force spectroscopy, single-cell electrophysiology
Topics:
Cells
Cite this article:
Li M, Liu L, Zambelli T. FluidFM for single-cell biophysics. Nano Research, 2022, 15(2): 773-786. https://doi.org/10.1007/s12274-021-3573-y
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Abstract Full text About this article

Fluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.


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About this article

FluidFM for single-cell biophysics

Show Author's information Mi Li1,2,3( ), Lianqing Liu2,3, Tomaso Zambelli1
Laboratory of Biosensors and BioelectronicsInstitute for Biomedical Engineering, ETH ZürichZürich8092Switzerland
State Key Laboratory of Robotics, Shenyang Institute of AutomationChinese Academy of SciencesShenyang110016China
Institutes for Robotics and Intelligent ManufacturingChinese Academy of SciencesShenyang110169China

Abstract

Fluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.

Keywords: atomic force microscopy, fluidic force microscopy, single-cell manipulation, single-cell force spectroscopy, single-cell electrophysiology

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Publication history
Copyright
Acknowledgements

Publication history

Received: 04 January 2021
Revised: 16 April 2021
Accepted: 05 May 2021
Published: 22 June 2021
Issue date: February 2022

Copyright

© The Author(s) 2021

Acknowledgements

Acknowledgements

This work was supported by the China Scholarship Council (CSC) (No. 202004910157). M. Li is thankful for the support from the National Natural Science Foundation of China (Nos. 61922081 and 61873258), the Key Research Program of Frontier Sciences CAS (No. ZDBS-LY-JSC043), the Youth Innovation Promotion Association CAS (No. 2017243), and the LiaoNing Revitalization Talents Program (No. XLYC1907072).

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