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Nano Research 2021, 14(4): 1087-1094 https://doi.org/10.1007/s12274-020-3153-6
Research Article | Issue | Published: 06 November 2020

In situ self-assembly of near-infrared-emitting gold nanoparticles into body-clearable 1D nanostructures with rapid lysosome escape and fast cellular excretion

Show Author's Information Kui He,  Jiayi Zhu,  Lingshan Gong,  Yue Tan,  Huarui Chen,  Huarun Liang,  Baihao Huang,  Jinbin Liu( )
Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China
Keywords:
self-assembly, gene delivery, luminescent gold nanoparticle, intracellular imaging, body clearance
Topics:
Cell cultureDNA GenesGold nanoparticlesMolecular biologyMolecular imagingNanoparticlesProteinsSelf assembly
Cite this article:
He K, Zhu J, Gong L, et al. In situ self-assembly of near-infrared-emitting gold nanoparticles into body-clearable 1D nanostructures with rapid lysosome escape and fast cellular excretion. Nano Research, 2021, 14(4): 1087-1094. https://doi.org/10.1007/s12274-020-3153-6
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Abstract Full text Electronic supplementary material About this article

The integration of strong near-infrared (NIR) emission, rapid lysosome escape, fast cellular excretion, and efficient total body clearance is highly desired for nanoparticles (NPs) to achieve synergistic functions in both molecular imaging and delivery. Herein, using a well-designed cyclopeptide (CP) that can spontaneously assemble into controllable nanofibers as template, a facile strategy is reported for in situ self-assembly of NIR-emitting gold NPs (AuNPs) into ordered and well-controlled one-dimensional (1D) nanostructures (AuNPs@CP) with greatly enhanced NIR emission (~ 6 fold). Comparing with the unassembled AuNPs, the AuNPs@CP are observed to enter living cells through endocytosis, escape from lysosome rapidly, and excrete the cell fast, which shows high gene transfection efficiencies in construction of cell line with ~ 7.5-fold overexpression of p53 protein. Furthermore, the AuNPs@CP exhibit high in vivo diffusibility and total body clearance efficiency with minimized healthy organ retention, which are also demonstrated to be good nanovectors for plasmid complementary deoxyribonucleic acid 3.1 (pcDNA3.1)(+)-internal ribosome entry site (IRES)-green fluorescent protein (GFP)-p53 plasmid with efficient p53 gene over-expression in tumor site. This facile in situ strategy in fabricating highly luminescent 1D nanostructures provides a promising approach toward future translatable multifunctional nanostructures for delivering, tracking, and therapy.


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Abstract
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Electronic supplementary material
About this article

In situ self-assembly of near-infrared-emitting gold nanoparticles into body-clearable 1D nanostructures with rapid lysosome escape and fast cellular excretion

Show Author's information Kui He, Jiayi Zhu, Lingshan Gong, Yue Tan, Huarui Chen, Huarun Liang, Baihao Huang, Jinbin Liu( )
Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China

Abstract

The integration of strong near-infrared (NIR) emission, rapid lysosome escape, fast cellular excretion, and efficient total body clearance is highly desired for nanoparticles (NPs) to achieve synergistic functions in both molecular imaging and delivery. Herein, using a well-designed cyclopeptide (CP) that can spontaneously assemble into controllable nanofibers as template, a facile strategy is reported for in situ self-assembly of NIR-emitting gold NPs (AuNPs) into ordered and well-controlled one-dimensional (1D) nanostructures (AuNPs@CP) with greatly enhanced NIR emission (~ 6 fold). Comparing with the unassembled AuNPs, the AuNPs@CP are observed to enter living cells through endocytosis, escape from lysosome rapidly, and excrete the cell fast, which shows high gene transfection efficiencies in construction of cell line with ~ 7.5-fold overexpression of p53 protein. Furthermore, the AuNPs@CP exhibit high in vivo diffusibility and total body clearance efficiency with minimized healthy organ retention, which are also demonstrated to be good nanovectors for plasmid complementary deoxyribonucleic acid 3.1 (pcDNA3.1)(+)-internal ribosome entry site (IRES)-green fluorescent protein (GFP)-p53 plasmid with efficient p53 gene over-expression in tumor site. This facile in situ strategy in fabricating highly luminescent 1D nanostructures provides a promising approach toward future translatable multifunctional nanostructures for delivering, tracking, and therapy.

Keywords: self-assembly, gene delivery, luminescent gold nanoparticle, intracellular imaging, body clearance

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Publication history
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Acknowledgements

Publication history

Received: 27 July 2020
Revised: 29 September 2020
Accepted: 01 October 2020
Published: 06 November 2020
Issue date: April 2021

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21573078 and 22022403), Guangdong Natural Science Funds for Distinguished Young Scholars (No. 2016A030306024), Guangzhou Science and Technology Project (No. 201904010055), and Fundamental Research Funds for the Central Universities.

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