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Nano Research 2021, 14(3): 770-777 https://doi.org/10.1007/s12274-020-3112-2
Research Article | Issue | Published: 01 March 2021

Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo

Show Author's Information Chang Lei1,§,  Yuxue Cao1,2,§,  Sepanta Hosseinpour2,  Fang Gao1,  Jingyu Liu3,  Jianye Fu1,  Reuben Staples2,  Saso Ivanovski2( ),  Chun Xu2( )
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia
School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia
School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology (QUT), Brisbane, QLD 4001, Australia

§ Chang lei and Yuxue Cao contribute equally to this work.

Keywords:
hydroxyapatite, tissue engineering, scaffolds, dendritic mesoporous silica nanoparticles
Topics:
BiocompatibilityDefectsNanoparticlesSilicaSilica nanoparticlesTissue regeneration
Cite this article:
Lei C, Cao Y, Hosseinpour S, et al. Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo. Nano Research, 2021, 14(3): 770-777. https://doi.org/10.1007/s12274-020-3112-2
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Abstract Full text Electronic supplementary material About this article

Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity. Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles (HA-DMSN) based scaffolds with hierarchical micro-pores (5 µm) and nano-pores (6.4 nm), and their application for bone regeneration. The in vitro studies demonstrated good biocompatibility of dissolution extracts, as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression (osteocalcin gene (OCN), osteopontin gene (OPN), collagen type I alpha 1 gene (CoL1A1), runt-related transcription factor 2 gene (RUNX2), and integrin-binding sialoprotein gene (IBSP)), alkaline phosphatise (ALP) activity, and alizarin red staining. The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing. Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering.


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Electronic supplementary material
About this article

Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo

Show Author's information Chang Lei1,§, Yuxue Cao1,2,§, Sepanta Hosseinpour2, Fang Gao1, Jingyu Liu3, Jianye Fu1, Reuben Staples2, Saso Ivanovski2( ), Chun Xu2( )
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia
School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia
School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology (QUT), Brisbane, QLD 4001, Australia

§ Chang lei and Yuxue Cao contribute equally to this work.

Abstract

Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity. Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles (HA-DMSN) based scaffolds with hierarchical micro-pores (5 µm) and nano-pores (6.4 nm), and their application for bone regeneration. The in vitro studies demonstrated good biocompatibility of dissolution extracts, as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression (osteocalcin gene (OCN), osteopontin gene (OPN), collagen type I alpha 1 gene (CoL1A1), runt-related transcription factor 2 gene (RUNX2), and integrin-binding sialoprotein gene (IBSP)), alkaline phosphatise (ALP) activity, and alizarin red staining. The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing. Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering.

Keywords: hydroxyapatite, tissue engineering, scaffolds, dendritic mesoporous silica nanoparticles

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Publication history
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Acknowledgements

Publication history

Received: 06 May 2020
Revised: 09 September 2020
Accepted: 12 September 2020
Published: 01 March 2021
Issue date: March 2021

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature

Acknowledgements

The authors acknowledge the support from University of Queensland (UQ) Early Career Researcher Grant (No. 1717673). C. Lei acknowledges the support of Advanced Queensland Fellowship. C. Xu acknowledges the support of National Health & Medical Research Council of Australia (NHMRC) Early Career Fellowship. S. Hosseinpour acknowledges the support of The University of Queensland International (UQI) Scholarship. The authors acknowledge the supports from the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, the University of Queensland.

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