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Nano Research 2021, 14(3): 707-714 https://doi.org/10.1007/s12274-020-3101-5
Research Article | Issue | Published: 01 March 2021

A mediator-free self-powered glucose biosensor based on a hybrid glucose/MnO2 enzymatic biofuel cell

Show Author's Information Shuai Hao1,2,  He Zhang1,2,  Xiaoxuan Sun1,2,  Junfeng Zhai1( ),  Shaojun Dong1,2( )
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
Keywords:
hydrogen peroxide, self-powered glucose biosensor, glucose oxidase, mediator-free, capacitive cathode
Topics:
AnodesCathodesChemical sensors Enzymatic fuel cellsGlucoseGlucose oxidaseGlucose sensors Manganese oxide
Cite this article:
Hao S, Zhang H, Sun X, et al. A mediator-free self-powered glucose biosensor based on a hybrid glucose/MnO2 enzymatic biofuel cell. Nano Research, 2021, 14(3): 707-714. https://doi.org/10.1007/s12274-020-3101-5
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Abstract Full text Electronic supplementary material About this article

Self-powered glucose biosensor (SPGB) is of great interest due to the advantages including single configuration, good stability and particularly no need of external power sources. Herein, a mediator-free SPGB with high sensitivity and good selectivity is constructed based on a hybrid enzymatic biofuel cell (EBFC) composed of a glucose oxidase/cobalt phthalocyanine/1-pyrenebutyric acid/ buckypaper (GOD/CoPc/PBA/BP) bioanode and a MnO2/PBA/BP capacitive cathode. The efficient electron transfer from GOD to electrodes is achieved successfully through the anode oxidation of hydrogen peroxide (H2O2), one nature product of glucose oxidation catalyzed by GOD, thus avoiding the potential drawbacks posed by the use of redox mediators. CoPc servers as an efficient catalyst to lower the anode potential required by the reaction of H2O2 to 0.17 V. The MnO2/PBA/BP capacitive cathode is utilized because it can not only provide a high discharge potential and adequate capacitance to match the bioanode well, but also exhibit no potential interference to the anodic reaction. The concentration of glucose can be detected simply by measuring the output of the SPGB and a wide linear detection range from 0.5 to 8 mM has been obtained with high sensitivities of 48.66 and 32.12 μA·cm-2·mM-1 with and without stirring, respectively. The recoveries of glucose in grape juice and human serum are in the range from 99.5% to 101.2% with the relative standard deviation (RSD) less than 8%, indicating the good promise of the SPGB in sensing glucose in real samples.


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Electronic supplementary material
About this article

A mediator-free self-powered glucose biosensor based on a hybrid glucose/MnO2 enzymatic biofuel cell

Show Author's information Shuai Hao1,2, He Zhang1,2, Xiaoxuan Sun1,2, Junfeng Zhai1( ), Shaojun Dong1,2( )
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China

Abstract

Self-powered glucose biosensor (SPGB) is of great interest due to the advantages including single configuration, good stability and particularly no need of external power sources. Herein, a mediator-free SPGB with high sensitivity and good selectivity is constructed based on a hybrid enzymatic biofuel cell (EBFC) composed of a glucose oxidase/cobalt phthalocyanine/1-pyrenebutyric acid/ buckypaper (GOD/CoPc/PBA/BP) bioanode and a MnO2/PBA/BP capacitive cathode. The efficient electron transfer from GOD to electrodes is achieved successfully through the anode oxidation of hydrogen peroxide (H2O2), one nature product of glucose oxidation catalyzed by GOD, thus avoiding the potential drawbacks posed by the use of redox mediators. CoPc servers as an efficient catalyst to lower the anode potential required by the reaction of H2O2 to 0.17 V. The MnO2/PBA/BP capacitive cathode is utilized because it can not only provide a high discharge potential and adequate capacitance to match the bioanode well, but also exhibit no potential interference to the anodic reaction. The concentration of glucose can be detected simply by measuring the output of the SPGB and a wide linear detection range from 0.5 to 8 mM has been obtained with high sensitivities of 48.66 and 32.12 μA·cm-2·mM-1 with and without stirring, respectively. The recoveries of glucose in grape juice and human serum are in the range from 99.5% to 101.2% with the relative standard deviation (RSD) less than 8%, indicating the good promise of the SPGB in sensing glucose in real samples.

Keywords: hydrogen peroxide, self-powered glucose biosensor, glucose oxidase, mediator-free, capacitive cathode

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Publication history
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Acknowledgements

Publication history

Received: 09 July 2020
Revised: 07 September 2020
Accepted: 08 September 2020
Published: 01 March 2021
Issue date: March 2021

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature

Acknowledgements

This work was supported by the National Natural Science Foundation of China (nos. 21721003 and 21675151) and the Ministry of Science and Technology of China (no. 2016YFA0203203).

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