Journal Home > Volume 14 , issue 3

Matrix adaptation reconstructs the architecture of cell, and has important implication in proper biological functioning. However, how the network of cytoskeleton filament undergoes reconstruction, reordering, and surface adaptation, requires a systematic investigation. Here, we show that surface sensing and adaptation occur correspondingly with related reorganization of cytoskeleton filaments (actin, tubulin, and vimentin). The microstructure of filament network is built by adaptive change of chemical polymerization on cytoskeleton filaments. The transition of cellular morphology, from spheroidal architecture on nanoarray to extending structure with stress fibers on flat surface, involves spatial reorganization and polymerization modulation of filaments. The dimension of filaments (diameter, orientation, and density) are changed accordingly to spatiotemporal distribution of cytoskeleton network. In addition, our findings elucidate how cell can tune their architecture at nanoscale by matrix adaptation, and provide a novel information on interplay between cytoskeleton and pathophysiology.

File
12274_2020_3076_MOESM1_ESM.pdf (2.5 MB)
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 10 June 2020
Revised: 28 July 2020
Accepted: 26 August 2020
Published: 01 March 2021
Issue date: March 2021

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature

Acknowledgements

This research was supported by the ShanghaTech University (Nos. 2013F020100001, 2014X020150110, and 2015X02015012). The authors thank the support from Analytical Instrumentation Center (No. SPST-AIC10112914), center for High-Resolution Electron Microscope (CħEM, No. EM02161943), SPST, ShanghaiTech University. We acknowledge the Joint Biological Super-Resolution Imaging Facility of ShanghaiTech University and Dr. Cuiping Tian for her help of image processing and analysis. We acknowledge Zikang Qin (Institute of Applied Physics and Computational Mathematics, Beijing) for his guidance in filament analysis work by MATLAB software. We acknowledge proteomic analysis from National Center for Protein Science Shanghai (NSPSS) at Shanghai, for the guidance of proteomic and IPA analysis.

Rights and permissions

Reprints and Permission requests may be sought directly from editorial office.
Email: nanores@tup.tsinghua.edu.cn

Return