Journal Home > Volume 13 , Issue 12
Nano Research 2020, 13(12): 3364-3370 https://doi.org/10.1007/s12274-020-3023-2
Research Article | Issue | Published: 12 September 2020

Multifunctional shape-dependent plasmonic nanoprobe by enzymatic etching of single gold triangular nanoplate

Show Author's Information Ning Feng1,§,  Jingjing Shen1,§,  Yu Chen1,  Chang Li1,  Yanling Hu1,2,  Lei Zhang1( ),  Shufen Chen1,  Quli Fan1,  Wei Huang1,3,  Lianhui Wang1( )
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China
School of Electrical and Control, Nanjing Polytechnic Institute, 188 Xinle Road, Nanjing 211500, China
Frontiers Science Center for Flexible Electronics (FSCFE), Shaanxi Institute of Flexible Electronics (SIFE) and Shaanxi Institute of Biomedical Materials and Engineering (SIBME), Northwestern Polytechnical University (NPU), 127 West Youyi Road, Xi'an 710072, China

§ Ning Feng and Jingjing Shen contributed equally to this work.

Keywords:
H2O2 detection, plasmonic, gold triangular nanoprisms, enzymatic etching, logic operations
Topics:
Coordination reactionsEtchingGoldGold compoundsHydrogen peroxideNanoprobesNanostructuresOxidationPeroxidesPlasmonics
Cite this article:
Feng N, Shen J, Chen Y, et al. Multifunctional shape-dependent plasmonic nanoprobe by enzymatic etching of single gold triangular nanoplate. Nano Research, 2020, 13(12): 3364-3370. https://doi.org/10.1007/s12274-020-3023-2
Download citation
EndNote(RIS)
BibTeX

623

Views

52

Downloads

Citations

20

Crossref

N/A

WoS

17

Scopus

1

CSCD

Abstract Full text Electronic supplementary material About this article

Hydrogen peroxide (H2O2), as a signaling molecule, plays a vital role in a wide variety of signaling transduction processes, aging, and diseases. However, the excessive production of H2O2 causes various diseases. Herein, we develop a novel method for H2O2 detection in live cells via dark-field scattering spectroscopy with gold triangular nanoprisms (AuTNPs) as probes. The corners of AuTNPs would be gradually oxidatively etched by the strong coordination of Br• which is generated by enzymatic reactions in the presence of horseradish peroxidase (HRP), bromide ion and trace hydrogen peroxide. Benefitting from the morphological change, the single AuTNP based plasmonic nanoprobe shows notable blueshifts and scattering color changes which could be real-time monitored under the dark-field microscopy. The peak position in the scattering spectra of individual AuTNP blueshifts linearly with the increase of H2O2 concentration, and exhibits high sensitivity to H2O2 in a large range from 2.5 to 100 μM with a low detection limit (LOD) of 0.74 μM. Moreover, the experimental results were supported by the simulated results via the finite-difference time-domain (FDTD) method. The nanoprobes have been further used for intracellular H2O2 detection in live cells. Besides, the etching of AuTNP also provides an alternative method to design novel plasmonic logic chips and write-once plasmonic memories.


menu
Abstract
Full text
Outline
Electronic supplementary material
About this article

Multifunctional shape-dependent plasmonic nanoprobe by enzymatic etching of single gold triangular nanoplate

Show Author's information Ning Feng1,§, Jingjing Shen1,§, Yu Chen1, Chang Li1, Yanling Hu1,2, Lei Zhang1( ), Shufen Chen1, Quli Fan1, Wei Huang1,3, Lianhui Wang1( )
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China
School of Electrical and Control, Nanjing Polytechnic Institute, 188 Xinle Road, Nanjing 211500, China
Frontiers Science Center for Flexible Electronics (FSCFE), Shaanxi Institute of Flexible Electronics (SIFE) and Shaanxi Institute of Biomedical Materials and Engineering (SIBME), Northwestern Polytechnical University (NPU), 127 West Youyi Road, Xi'an 710072, China

§ Ning Feng and Jingjing Shen contributed equally to this work.

Abstract

Hydrogen peroxide (H2O2), as a signaling molecule, plays a vital role in a wide variety of signaling transduction processes, aging, and diseases. However, the excessive production of H2O2 causes various diseases. Herein, we develop a novel method for H2O2 detection in live cells via dark-field scattering spectroscopy with gold triangular nanoprisms (AuTNPs) as probes. The corners of AuTNPs would be gradually oxidatively etched by the strong coordination of Br• which is generated by enzymatic reactions in the presence of horseradish peroxidase (HRP), bromide ion and trace hydrogen peroxide. Benefitting from the morphological change, the single AuTNP based plasmonic nanoprobe shows notable blueshifts and scattering color changes which could be real-time monitored under the dark-field microscopy. The peak position in the scattering spectra of individual AuTNP blueshifts linearly with the increase of H2O2 concentration, and exhibits high sensitivity to H2O2 in a large range from 2.5 to 100 μM with a low detection limit (LOD) of 0.74 μM. Moreover, the experimental results were supported by the simulated results via the finite-difference time-domain (FDTD) method. The nanoprobes have been further used for intracellular H2O2 detection in live cells. Besides, the etching of AuTNP also provides an alternative method to design novel plasmonic logic chips and write-once plasmonic memories.

Keywords: H2O2 detection, plasmonic, gold triangular nanoprisms, enzymatic etching, logic operations

References(47)

[1]
M. B. Azad,; Y. Q. Chen,; S. B. Gibson, Regulation of autophagy by reactive oxygen species (ROS): Implications for cancer progression and treatment. Antioxid Redox Signal. 2009, 11, 777-790.
DOI Google Scholar
[2]
Y. Nosaka,; A. Y. Nosaka, Generation and detection of reactive oxygen species in photocatalysis. Chem. Rev. 2017, 117, 11302-11336.
DOI Google Scholar
[3]
D. Hernández-García,; C. D. Wood,; S. Castro-Obregón,; L. Covarrubias, Reactive oxygen species: A radical role in development? Free Radic. Biol. Med. 2010, 49, 130-143.
DOI Google Scholar
[4]
M. Giorgio,; M. Trinei,; E. Migliaccio,; P. G. Pelicci, Hydrogen peroxide: A metabolic by-product or a common mediator of ageing signals? Nat. Rev. Mol. Cell. Biol. 2007, 8, 722-728.
DOI Google Scholar
[5]
T. Finkel,; N. J. Holbrook, Oxidants, oxidative stress and the biology of ageing. Nature 2000, 408, 239-247.
DOI Google Scholar
[6]
S. G. Rhee,; S. W. Kang,; W. Jeong,; T. S. Chang,; K. S. Yang,; H. A. Woo, Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Curr. Opin. Cell Biol. 2005, 17, 183-189.
DOI Google Scholar
[7]
N. V. Klassen,; D. Marchington,; H. C. E. McGowan, H2O2 determination by the I3-method and by KMnO4 titration. Anal. Chem. 1994, 66, 2921-2925.
DOI Google Scholar
[8]
M. L. Lian,; X. Chen,; Y. L. Lu,; W. S. Yang, Self-assembled peptide hydrogel as a smart biointerface for enzyme-based electrochemical biosensing and cell monitoring. ACS Appl. Mater. Interfaces 2016, 8, 25036-25042.
DOI Google Scholar
[9]
S. K. Maji,; S. Sreejith,; A. K. Mandal,; X. Ma,; Y. L. Zhao, Immobilizing gold nanoparticles in mesoporous silica covered reduced graphene oxide: A hybrid material for cancer cell detection through hydrogen peroxide sensing. ACS Appl. Mater. Interfaces 2014, 6, 13648-13656.
DOI Google Scholar
[10]
M. G. Ren,; B. B. Deng,; K. Zhou,; X. Q. Kong,; J. Y. Wang,; W. Y. Lin, Single fluorescent probe for dual-imaging viscosity and H2O2 in mitochondria with different fluorescence signals in living cells. Anal. Chem. 2017, 89, 552-555.
DOI Google Scholar
[11]
C. C. Gao,; Y. Tian,; R. B. Zhang,; J. Jing,; X. L. Zhang, Endoplasmic reticulum-directed ratiometric fluorescent probe for quantitive detection of basal H2O2. Anal. Chem. 2017, 89, 12945-12950.
Google Scholar
[12]
X. Y. Jiang,; H. J. Wang,; R. Yuan,; Y. Q. Chai, Functional three-dimensional porous conductive polymer hydrogels for sensitive electrochemiluminescence in situ detection of H2O2 released from live cells. Anal. Chem. 2018, 90, 8462-8469.
Google Scholar
[13]
Y. Wen,; K. Y. Liu,; H. R. Yang,; Y. Li,; H. C. Lan,; Y. Liu,; X. Y. Zhang,; T. Yi, A highly sensitive ratiometric fluorescent probe for the detection of cytoplasmic and nuclear hydrogen peroxide. Anal. Chem. 2014, 86, 9970-9976.
Google Scholar
[14]
G. Masanta,; C. H. Heo,; C. S. Lim,; S. K. Bae,; B. R. Cho,; H. M. Kim, A mitochondria-localized two-photon fluorescent probe for ratiometric imaging of hydrogen peroxide in live tissue. Chem. Commun. 2012, 48, 3518-3520.
Google Scholar
[15]
J. Xu,; Y. Zhang,; H. Yu,; X. D. Gao,; S. J. Shao, Mitochondria-targeted fluorescent probe for imaging hydrogen peroxide in living cells. Anal. Chem. 2016, 88, 1455-1461.
Google Scholar
[16]
P. F. Gao,; Y. F. Li,; C. Z. Huang, Localized surface plasmon resonance scattering imaging and spectroscopy for real-time reaction monitoring. Appl. Spectrosc. Rev. 2019, 54, 237-249.
Google Scholar
[17]
X. M. Ma,; S. He,; B. Qiu,; F. Luo,; L. H. Guo,; Z. Y. Lin, Noble metal nanoparticle-based multicolor immunoassays: An approach toward visual quantification of the analytes with the naked eye. ACS Sens. 2019, 4, 782-791.
Google Scholar
[18]
H. H. Rao,; X. Xue,; H. Q. Wang,; Z. H. Xue, Gold nanorod etching-based multicolorimetric sensors: Strategies and applications. J. Mater. Chem. C 2019, 7, 4610-4621.
Google Scholar
[19]
H. T. Yang,; A. R. Liu,; M. Wei,; Y. J. Liu,; B. J. Lv,; W. Wei,; Y. J. Zhang,; S. Q. Liu, Visual, label-free telomerase activity monitor via enzymatic etching of gold nanorods. Anal. Chem. 2017, 89, 12094-12100.
Google Scholar
[20]
Z. H. Chen,; C. Q. Chen,; H. W. Huang,; F. Luo,; L. H. Guo,; L. Zhang,; Z. Y. Lin,; G. N. Chen, Target-induced horseradish peroxidase deactivation for multicolor colorimetric assay of hydrogen sulfide in rat brain microdialysis. Anal. Chem. 2018, 90, 6222-6228.
Google Scholar
[21]
J. Cheng,; Y. Liu,; X. D. Cheng,; Y. He,; E. S. Yeung, Real time observation of chemical reactions of individual metal nanoparticles with high-throughput single molecule spectral microscopy. Anal. Chem. 2010, 82, 8744-8749.
Google Scholar
[22]
T. Xie,; C. Jing,; W. Ma,; Z. F. Ding,; A. J. Gross,; Y. T. Long, Real-time monitoring for the morphological variations of single gold nanorods. Nanoscale 2015, 7, 511-517.
Google Scholar
[23]
S. S. Sun,; M. X. Gao,; G. Lei,; H. Y. Zou,; J. Ma,; C. Z. Huang, Visually monitoring the etching process of gold nanoparticles by kI/I2 at single-nanoparticle level using scattered-light dark-field microscopic imaging. Nano Res. 2016, 9, 1125-1134.
Google Scholar
[24]
Z. X. Chen,; J. J. Li,; X. Q. Chen,; J. T. Cao,; J. R. Zhang,; Q. H. Min,; J. J. Zhu, Single gold@silver nanoprobes for real-time tracing the entire autophagy process at single-cell level. J. Am. Chem. Soc. 2015, 137, 1903-1908.
Google Scholar
[25]
G. Bodelón,; C. Costas,; J. Pérez-Juste,; I. Pastoriza-Santos,; L. M. Liz-Marzán, Gold nanoparticles for regulation of cell function and behavior. Nano Today. 2017, 13, 40-60.
Google Scholar
[26]
A. M. Azevedo,; V. C. Martins,; D. M. F. Prazeres,; V. Vojinović,; J. M. S. Cabral,; L. P. Fonseca, Horseradish peroxidase: A valuable tool in biotechnology. Biotechnol. Ann. Rev. 2003, 9, 199-247.
Google Scholar
[27]
L. Saa,; M. Coronado-Puchau,; V. Pavlov,; L. M. Liz-Marzán, Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection. Nanoscale 2014, 6, 7405-7409.
Google Scholar
[28]
Y. J. Huang,; A. R. Ferhan,; Y. Gao,; A. Dandapat,; D. H. Kim, High-yield synthesis of triangular gold nanoplates with improved shape uniformity, tunable edge length and thickness. Nanoscale 2014, 6, 6496-6500.
Google Scholar
[29]
L. Scarabelli,; M. Coronado-Puchau,; J. J. Giner-Casares,; J. Langer,; L. M. Liz-Marzán, Monodisperse gold nanotriangles: Size control, large-scale self-assembly, and performance in surface-enhanced Raman scattering. ACS Nano 2014, 8, 5833-5842.
Google Scholar
[30]
L. Chen,; F. Ji,; Y. Xu,; L. He,; Y. F. Mi,; F. Bao,; B. Q. Sun,; X. H. Zhang,; Q. Zhang, High-yield seedless synthesis of triangular gold nanoplates through oxidative etching. Nano Lett. 2014, 14, 7201-7206.
Google Scholar
[31]
G. Chandrasekar,; K. Mougin,; H. Haidara,; L. Vidal,; E. Gnecco, Shape and size transformation of gold nanorods (GNRs) via oxidation process: A reverse growth mechanism. Appl. Surf. Sci. 2011, 257, 4175-4179.
Google Scholar
[32]
Q. N. Zhu,; J. Wu,; J. W. Zhao,; W. H. Ni, Role of bromide in hydrogen peroxide oxidation of CTAB-stabilized gold nanorods in aqueous solutions. Langmuir 2015, 31, 4072-4077.
Google Scholar
[33]
W. H. Ni,; X. S. Kou,; Z. Yang,; J. F. Wang, Tailoring longitudinal surface plasmon wavelengths, scattering and absorption cross sections of gold nanorods. ACS Nano 2008, 2, 677-686.
Google Scholar
[34]
G. J. Weng,; X. J. Dong,; J. J. Li,; J. W. Zhao, Halide ions can trigger the oxidative etching of gold nanorods with the iodide ions being the most efficient. J. Mater. Sci. 2016, 51, 7678-7690.
Google Scholar
[35]
M. J. Männel,; L. P. Kreuzer,; C. Goldhahn,; J. Schubert,; M. J. Hartl,; M. Chanana, Catalytically active protein coatings: Toward enzymatic cascade reactions at the intercolloidal level. ACS Catal. 2017, 7, 1664-1672.
Google Scholar
[36]
A. L. Crumbliss,; S. C. Perine,; J. Stonehuerner,; K. R. Tubergen,; J. G. Zhao,; R. W. Henkens,; J. P. O'Daly, Colloidal gold as a biocompatible immobilization matrix suitable for the fabrication of enzyme electrodes by electrodeposition. Biotechnol. Bioeng. 1992, 40, 483-490.
Google Scholar
[37]
M. X. Zhang,; B. H. Huang,; X. Y. Sun,; D. W. Pang, Clickable gold nanoparticles as the building block of nanobioprobes. Langmuir 2010, 26, 10171-10176.
Google Scholar
[38]
X. Guo,; Q. Zhang,; Y. H. Sun,; Q. Zhao,; J. Yang, Lateral etching of core-shell Au@metal nanorods to metal-tipped Au nanorods with improved catalytic activity. ACS Nano 2012, 6, 1165-1175.
Google Scholar
[39]
J. An,; B. Tang,; X. H. Ning,; J. Zhou,; B. Zhao,; W. Q. Xu,; C. Corredor,; J. R. Lombardi, Photoinduced shape evolution:  From triangular to hexagonal silver nanoplates. J. Phys. Chem. C 2007, 111, 18055-18059.
Google Scholar
[40]
B. Xiong,; R. Zhou,; J. R. Hao,; Y. H. Jia,; Y. He,; E. S. Yeung, Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles. Nat. Commun. 2013, 4, 1708.
Google Scholar
[41]
G. Wang,; S. Y. Tao,; Y. D. Liu,; L. Guo,; G. H. Qin,; K. Ijiro,; M. Maeda,; Y. D. Yin, High-yield halide-free synthesis of biocompatible Au nanoplates. Chem. Commun. 2016, 52, 398-401.
Google Scholar
[42]
N. Y. Chen,; Y. J. Zhang,; H. Y. Liu,; H. M. Ruan,; C. Dong,; Z. Y. Shen,; A. G. Wu, A supersensitive probe for rapid colorimetric detection of nickel ion based on a sensing mechanism of anti-etching. ACS Sustainable Chem. Eng. 2016, 4, 6509-6516.
Google Scholar
[43]
Y. Y. Ni,; J. Li,; Z. Z. Huang,; K. He,; J. Q. Zhuang,; W. S. Yang, Improved activity of immobilized horseradish peroxidase on gold nanoparticles in the presence of bovine serum albumin. J. Nanopart. Res. 2013, 15, 2038.
Google Scholar
[44]
K. M. Geraghty,; S. Chen,; J. E. Harthill,; A. F. Ibrahim,; R. Toth,; N. A. Morrice,; F. Vandermoere,; G. B. Moorhead,; D. G. Hardie,; C. MacKintosh, Regulation of multisite phosphorylation and 14-3-3 binding of as160 in response to IGF-1, EGF, PMA and AICAR. Biochem. J. 2007, 407, 231-241.
Google Scholar
[45]
C. X. Wang,; Y. Du,; Q. Wu,; S. G. Xuan,; J. J. Zhou,; J. B. Song,; F. W. Shao,; H. W. Duan, Stimuli-responsive plasmonic core-satellite assemblies: I-motif DNA linker enabled intracellular pH sensing. Chem. Commun. 2013, 49, 5739-5741.
Google Scholar
[46]
C. Jing,; Z. Gu,; Y. L. Ying,; D. W. Li,; L. Zhang,; Y. T. Long, Chrominance to dimension: A real-time method for measuring the size of single gold nanoparticles. Anal. Chem. 2012, 84, 4284-4291.
Google Scholar
[47]
Y. Zhang,; Z. H. Shuai,; H. Zhou,; Z. M. Luo,; B. Liu,; Y. N. Zhang,; L. Zhang,; S. F. Chen,; J. Chao,; L. X. Weng, et al. Single-molecule analysis of microRNA and logic operations using a smart plasmonic nanobiosensor. J. Am. Chem. Soc. 2018, 140, 3988-3993.
Google Scholar
File
12274_2020_3023_MOESM1_ESM.pdf (2.8 MB)
Publication history
Copyright
Acknowledgements

Publication history

Received: 23 June 2020
Revised: 26 July 2020
Accepted: 31 July 2020
Published: 12 September 2020
Issue date: December 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature

Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (No. 2017YFA0205302), the National Natural Science Foundation of China (Nos. 61571239 and 21674048), Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R37), the Key Research and Development Program of Jiangsu (No. BE2018732).

Return