Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes

Jianxi Ke; Shan Lu; Zhuo Li; Xiaoying Shang; Xingjun Li; Renfu Li; Datao Tu; Zhuo Chen; Xueyuan Chen

doi:10.1007/s12274-020-2837-2

Nano Research 2020, 13(7): 1955-1961 https://doi.org/10.1007/s12274-020-2837-2

Research Article | Issue | Published: 22 May 2020

Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes

Show Author's Information Hide Author's Information Jianxi Ke^{¹^,²^,³}, Shan Lu^{¹^,³^,⁴}(

), Zhuo Li^¹, Xiaoying Shang^¹, Xingjun Li^¹, Renfu Li^¹, Datao Tu^{¹^,⁴}, Zhuo Chen^¹, Xueyuan Chen^{¹^,²^,³^,⁴}(

)

1CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China

2School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China

3University of Chinese Academy of Sciences, Beijing 100049, China

4Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou 350108, China

Keywords:

energy transfer, upconversion nanoparticles, dual-excitation, dye sensitization, ratiometric probe, multiplexed detection

Topics:

Chlorine compounds Infrared devices Nanoprobes Signal processing

Cite this article:

Ke J, Lu S, Li Z, et al. Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes. Nano Research, 2020, 13(7): 1955-1961. https://doi.org/10.1007/s12274-020-2837-2

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Abstract

Multiplexed intracellular detection is desirable in biomedical sciences for its higher efficiency and accuracy compared to the single-analyte detection. However, it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously. Herein, we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles (UCNP) loaded with two dyes for sensitization and quenching of the upconversion luminescence (UCL), respectively. Based on the two independent energy transfer processes of near-infrared (NIR) dye IR845 to UCNP and UCNP to visible dye PAPS-Zn, ClO^- and Zn²⁺ were simultaneously detected with a limit of detection (LOD) of 41.4 and 10.5 nM, respectively. By utilizing a purpose-built 830/980 nm dual-laser confocal microscope, both intrinsic and exogenous ClO^- and Zn²⁺ in live MCF-7 cells have been accurately quantified. Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells. Through modulation of NIR dye and visible dye with other reactive groups, the nanoprobes can be extended to analyze various intracellular species, which provides a promising tool to study the biological activities in live cells and diagnose diseases.

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Multiplexed intracellular detection based on dual-excitation/dual-emission upconversion nanoprobes

Show Author's information Hide Author's Information Jianxi Ke^{¹^,²^,³}, Shan Lu^{¹^,³^,⁴}(

), Zhuo Li^¹, Xiaoying Shang^¹, Xingjun Li^¹, Renfu Li^¹, Datao Tu^{¹^,⁴}, Zhuo Chen^¹, Xueyuan Chen^{¹^,²^,³^,⁴}(

)

2School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China

3University of Chinese Academy of Sciences, Beijing 100049, China

4Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou 350108, China

Abstract

Keywords: energy transfer, upconversion nanoparticles, dual-excitation, dye sensitization, ratiometric probe, multiplexed detection

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Publication history

Acknowledgements

Publication history

Received: 15 January 2020

Revised: 24 April 2020

Accepted: 28 April 2020

Published: 22 May 2020

Issue date: July 2020

Copyright

Acknowledgements

This work was supported by the Science and Technology Cooperation Fund between Chinese and Australian Governments (No. 2017YFE0132300), the Strategic Priority Research Program of the CAS (No. XDB20000000), the National Natural Science Foundation of China (Nos. 51672272, 21771185, 21771178, and 21975257), Youth Innovation Promotion Association of CAS (No. 2017347), and the CAS/SAFEA International Partnership Program for Creative Research Teams.