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Nano Research 2020, 13(8): 2156-2164 https://doi.org/10.1007/s12274-020-2824-7
Research Article | Issue | Published: 05 August 2020

Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets

Show Author's Information Wanshun Wang1,§,  Binglin Li1,§,  Huili Yang1,§,  Zefeng Lin1,  Lingling Chen1,  Zhan Li1,  Jiayuan Ge1,  Tao Zhang1,  Hong Xia1( ),  Lihua Li2( ),  Yao Lu1,3( )
Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, Guangzhou 510640, China
Orthopedic Centre, Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

§ Wanshun Wang, Binglin Li, and Huili Yang contributed equally to this work.

Keywords:
wound healing, nanozyme, multidrug-resistant bacteria, antibacterial nanomaterials
Topics:
Bacteria BiocompatibilityCytologyGrapheneNanoparticlesNanosheetsNanostructured materialsStaphylococcus aureusSulfur compoundsSynthesis (chemical)
Cite this article:
Wang W, Li B, Yang H, et al. Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets. Nano Research, 2020, 13(8): 2156-2164. https://doi.org/10.1007/s12274-020-2824-7
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Abstract Full text Electronic supplementary material About this article

Antibacterial nanomaterials have attracted growing interest for bacterial infection therapy. However, most nanomaterials eliminate bacteria either physically or chemically, which hampers their efficacy when dealing with multidrug-resistant bacteria. To overcome this, we integrated copper sulfide (CuS) nanoparticles with active graphene oxide nanosheets (GO NSs) to synthesize a superior nanocomposite (CuS/GO NC) that acts both physically and chemically on the bacteria. CuS/GO NC was produced using a facile hydrothermal method, whereby the CuS nanoparticles grew and were uniformly dispersed on the GO NSs in situ. We found that the CuS/GO NC possesses a unique needle-like morphology that physically damages the bacterial cell membrane. CuS/GO NC also exhibits high oxidase- and peroxidase-like activity, ensuring efficient generation of the reactive oxygen species •OH from H2O2, which kills bacteria chemically. These features endow the CuS/GO NC with excellent antibacterial capabilities to kill multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) with only a single dose. Additionally, it was found that the CuS/GO NC accelerated the healing of infected wounds in vivo owing to its good biocompatibility as well as facilitation of cell migration and collagen secretion. This study provides a new strategy to combine the physical and chemical antibacterial modes of nanomaterials to develop more effective therapies to combat multidrug-resistant bacterial infections.


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Electronic supplementary material
About this article

Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets

Show Author's information Wanshun Wang1,§, Binglin Li1,§, Huili Yang1,§, Zefeng Lin1, Lingling Chen1, Zhan Li1, Jiayuan Ge1, Tao Zhang1, Hong Xia1( ), Lihua Li2( ), Yao Lu1,3( )
Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theater Command of PLA, The Second Clinical Medical College and Department of Graduate School of Guangzhou University of Chinese Medicine, The Second School of Clinical Medicine of Southern Medical University, Guangzhou 510010, China
State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, Guangzhou 510640, China
Orthopedic Centre, Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

§ Wanshun Wang, Binglin Li, and Huili Yang contributed equally to this work.

Abstract

Antibacterial nanomaterials have attracted growing interest for bacterial infection therapy. However, most nanomaterials eliminate bacteria either physically or chemically, which hampers their efficacy when dealing with multidrug-resistant bacteria. To overcome this, we integrated copper sulfide (CuS) nanoparticles with active graphene oxide nanosheets (GO NSs) to synthesize a superior nanocomposite (CuS/GO NC) that acts both physically and chemically on the bacteria. CuS/GO NC was produced using a facile hydrothermal method, whereby the CuS nanoparticles grew and were uniformly dispersed on the GO NSs in situ. We found that the CuS/GO NC possesses a unique needle-like morphology that physically damages the bacterial cell membrane. CuS/GO NC also exhibits high oxidase- and peroxidase-like activity, ensuring efficient generation of the reactive oxygen species •OH from H2O2, which kills bacteria chemically. These features endow the CuS/GO NC with excellent antibacterial capabilities to kill multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) with only a single dose. Additionally, it was found that the CuS/GO NC accelerated the healing of infected wounds in vivo owing to its good biocompatibility as well as facilitation of cell migration and collagen secretion. This study provides a new strategy to combine the physical and chemical antibacterial modes of nanomaterials to develop more effective therapies to combat multidrug-resistant bacterial infections.

Keywords: wound healing, nanozyme, multidrug-resistant bacteria, antibacterial nanomaterials

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Publication history
Copyright
Acknowledgements

Publication history

Received: 20 March 2020
Revised: 10 April 2020
Accepted: 19 April 2020
Published: 05 August 2020
Issue date: August 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Nos. 81972080 and 81902198), China Postdoctoral Science Foundation (Nos. 2018M640776, 2019M662980, and BX20190150), Natural Science Foundation of Guangdong Province (Nos. 2015A030312004 and 2020A1515010398), Science and Technology Planning Project of Guangdong Province (Nos. 2014A020215025 and 2017B030314139), Medical Research Foundation of Guangdong Province (A2019228), Research Program of PLA (No. CGZ16C004), President Foundation of Zhujiang Hospital, Southern Medical University (No. yzjj2018rc09), and Scientific Research Foundation of Southern Medical University (Nos. C1051353 and PY2018N060).

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