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Nano Research 2020, 13(4): 1020-1028 https://doi.org/10.1007/s12274-020-2738-4
Research Article | Open Access | Issue | Published: 14 April 2020

Nanoscale image of the drug/metal mono-layer interaction: Tapping AFM-IR investigations

Show Author's Information Natalia Piergies1( ),  Alexandre Dazzi2,  Ariane Deniset-Besseau2,  Jérémie Mathurin2,  Magdalena Oćwieja3,  Czesława Paluszkiewicz1,  Wojciech M. Kwiatek1
Institute of Nuclear, Physics Polish Academy of Sciences, PL-31342 Krakow, Poland
Laboratoire de Chimie Physique (LCP), CNRS UMR 8000, Univ. of Paris-Sud, Université Paris-Saclay, 91405 Orsay, France
Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, PL-30239 Krakow, Poland
Keywords:
atomic force microscopy, metal nanoparticle mono-layer, drug’s adsorption, infrared nanospectroscopy, erlotinib
Topics:
Drug interactionsGold nanoparticlesInfrared spectroscopyMetal nanoparticlesMetalsSilver nanoparticlesSurface plasmon resonance
Cite this article:
Piergies N, Dazzi A, Deniset-Besseau A, et al. Nanoscale image of the drug/metal mono-layer interaction: Tapping AFM-IR investigations. Nano Research, 2020, 13(4): 1020-1028. https://doi.org/10.1007/s12274-020-2738-4
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Abstract Full text About this article

The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy development. However, this strategy requires precise information about how the drug interacts with the applied nanocarrier. In this study, atomic force microscopy combined with infrared spectroscopy (AFM-IR) was used for the first time to investigate the erlotinib adsorption structure on two different types of 15 nm metal nanoparticle mono-layers, namely, silver nanoparticle (AgNP) and gold nanoparticle (AuNP) mono-layers. Because the metal nanoparticles are loosely bound samples, only the tapping AFM-IR mode is suitable for the collection of IR maps and spectra for such a system. The obtained results indicated the relevance of the AFM-IR technique for characterizing drug interactions with a metal mono-layer surface. The investigated drug interacts with the AgNPs mainly through phenyl rings and methoxy moieties, while quinazoline, amino, and ethoxy moieties appear to be farther from the surface. For the AuNPs, the interaction occurs through both the phenyl ring and the quinazoline moiety. Additionally, the aliphatic groups of erlotinib directly participate in this interaction. The novelty of the present work is also related to the use of the tapping AFM-IR mode to study metal NP mono-layers with a drug adsorbed on them. The collected IR maps for the most enhanced erlotinib bands show specific areas with very high signal intensity. The connection between these areas and the "hot spots" typical for the surface plasmon resonance phenomenon of metals is considered.


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About this article

Nanoscale image of the drug/metal mono-layer interaction: Tapping AFM-IR investigations

Show Author's information Natalia Piergies1( ), Alexandre Dazzi2, Ariane Deniset-Besseau2, Jérémie Mathurin2, Magdalena Oćwieja3, Czesława Paluszkiewicz1, Wojciech M. Kwiatek1
Institute of Nuclear, Physics Polish Academy of Sciences, PL-31342 Krakow, Poland
Laboratoire de Chimie Physique (LCP), CNRS UMR 8000, Univ. of Paris-Sud, Université Paris-Saclay, 91405 Orsay, France
Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, PL-30239 Krakow, Poland

Abstract

The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy development. However, this strategy requires precise information about how the drug interacts with the applied nanocarrier. In this study, atomic force microscopy combined with infrared spectroscopy (AFM-IR) was used for the first time to investigate the erlotinib adsorption structure on two different types of 15 nm metal nanoparticle mono-layers, namely, silver nanoparticle (AgNP) and gold nanoparticle (AuNP) mono-layers. Because the metal nanoparticles are loosely bound samples, only the tapping AFM-IR mode is suitable for the collection of IR maps and spectra for such a system. The obtained results indicated the relevance of the AFM-IR technique for characterizing drug interactions with a metal mono-layer surface. The investigated drug interacts with the AgNPs mainly through phenyl rings and methoxy moieties, while quinazoline, amino, and ethoxy moieties appear to be farther from the surface. For the AuNPs, the interaction occurs through both the phenyl ring and the quinazoline moiety. Additionally, the aliphatic groups of erlotinib directly participate in this interaction. The novelty of the present work is also related to the use of the tapping AFM-IR mode to study metal NP mono-layers with a drug adsorbed on them. The collected IR maps for the most enhanced erlotinib bands show specific areas with very high signal intensity. The connection between these areas and the "hot spots" typical for the surface plasmon resonance phenomenon of metals is considered.

Keywords: atomic force microscopy, metal nanoparticle mono-layer, drug’s adsorption, infrared nanospectroscopy, erlotinib

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Publication history
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Publication history

Received: 05 January 2020
Revised: 25 February 2020
Accepted: 02 March 2020
Published: 14 April 2020
Issue date: April 2020

Copyright

© The Author(s) 2020

Acknowledgements

This work was supported by the National Science Centre Poland (No. 2016/21/D/ST4/02178 to N. P.). N. P. gratefully acknowledges the financial support of the French Government and the French Embassy in Poland. These researches were also supported by the Paris Ile-de-France Region-DIM Materiaux anciens et patrimoniaux. The measurements were partly performed using the equipment purchased in the frame of the project co-funded by the MałopolskaRegional Operational Program Measure 5.1 Krakow Metropolitan Areaas an important hub of the European Research Area for 2007-2013, project no. MRPO.05.01.00-12-013/15e.

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