Journal Home > Volume 13 , issue 3

Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization, yet it remains a great challenge due to limited insights into plant translocation mechanisms. Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis, whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals. Herein, we report gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for the spatiotemporal visualization of pesticide translocation in plant leaves. This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition. Using this MSI platform, we proposed the elaborate plant translocation mechanism of agrochemicals for the first time, which is currently poorly understood. The dynamic processes of carrier-mediated pesticides can be clearly visualized, including crossing of plasma membranes by transporters, translocation downward in stems through the phloem, diffusion to the xylem and, conversely, accumulation at margins of the treated leaves. Moreover, this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments, expediting researches across a broad range of fields, especially in nanomaterial development and life sciences.

File
12274_2020_2700_MOESM1_ESM.pdf (3.5 MB)
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 11 January 2020
Revised: 01 February 2020
Accepted: 05 February 2020
Published: 28 February 2020
Issue date: March 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020

Acknowledgements

We gratefully acknowledge the financial support from the National Natural Science Foundation of China (Nos. 31901911 and 21904142), the National Key R&D Program of China (No. 2018YFD0200300), and the Natural Science Foundation of Guangdong Province (No. 2018A030310215). This work is also supported by the project funded by the National Postdoctoral Program for Innovative Talents (No. BX20180399).

Rights and permissions

Reprints and Permission requests may be sought directly from editorial office.
Email: nanores@tup.tsinghua.edu.cn

Return