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Research Article

Specific photoacoustic cavitation through nucleus targeted nanoparticles for high-efficiency tumor therapy

Yuan Wang1,2,§, Guangle Niu3,4,§, Shaodong Zhai1,2, Wenjia Zhang1,2, Da Xing1,2( )
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China
College of Biophotonics, South China Normal University, Guangzhou 510631, China
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Department of Pharmacology & Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

§ Yuan Wang and Guangle Niu contributed equally to this work.

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Graphical Abstract

Abstract

As a new type of cancer treatment, photoacoustic (PA) therapy is based on PA shockwave for rapid, selective and effective killing of cancer cells. The nucleus has been widely used as a target for tumor therapy, which has obtained a very considerable therapeutic effect. In situ destruction of tumor cell nucleus by photoacoustic therapy has not been studied. In this paper, a highly efficient nucleus-targeted photoacoustic theranostic polymer was developed for fluorescence and photoacoustic dual-mode imaging-guided PA therapy. The prepared polymer consists of nucleus targeting TAT peptide (TAT: YGRKKRRQRRR), hydrophilic chain poly (N,N-dimethylacrylamide) (PDMA), and near-infrared (NIR) light absorbing agent (hCyR), which can self-assemble to form nanoparticles of approximately 28 nm (denoted as TAT-PDMA-hCyR NPs). The designed nanoparticles show excellent nucleus targeting and tumor cell death (up to 80%) caused by DNA damage under pulsed laser irradiation compared to non-nucleus target counterpart PDMA-hCyR NPs without TAT peptide in vitro. As expected, the fluorescence and PA dual-mode imaging observed that TAT-PDMA-hCyR NPs were able to passively target and enrich in tumors, providing an experimental basis for in vivo treatment and thus ensuring a significant tumor inhibition rate (about 92%). In conclusion, this study provides a new and practicable method for the development of nucleus-targeting nanoparticles as potential theranostic agent for in vivo cancer imaging and therapy.

Keywords

photoacoustic therapynucleusnear-infrared lighttargeted therapy

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Nano Research
Volume 13 Issue 3,
March 2020
Pages 719-728
DOI: 10.1007/s12274-020-2681-4
Cite this article:
Wang Y, Niu G, Zhai S, et al. Specific photoacoustic cavitation through nucleus targeted nanoparticles for high-efficiency tumor therapy. Nano Research, 2020, 13(3): 719-728. https://doi.org/10.1007/s12274-020-2681-4
Topics:
Cell death DiseasesFluorescenceInfrared devices Medical imagingNanoparticlesPeptidesTheranostics

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Received: 29 November 2019
Revised: 20 January 2020
Accepted: 22 January 2020
Published: 26 February 2020
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
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