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Nano Research 2020, 13(5): 1427-1433 https://doi.org/10.1007/s12274-020-2678-z
Research Article | Issue | Published: 20 February 2020

Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots

Show Author's Information Qi Xin1,§,  Xinrui Jia1,2,§,  Asmat Nawaz1,2,  Wenjing Xie1,  Litao Li3,  Jian Ru Gong1,2( )
CAS Center of Excellence for Nanoscience, CAS Key Laboratory for Nanosystem & Hierarchical Fabrication, National Center for Nanoscience and Technology, 11 Beiyitiao Zhongguancun, Beijing 100190, China
University of Chinese Academy of Sciences, Beijing 100049, China
Department of Orthopaedics, the 309th Hospital of the PLA, Beijing 100091, China

§ Qi Xin and Xinrui Jia contributed equally to this work.

Keywords:
graphene quantum dot, nanoenzyme, heme active site, peroxidase mimetic, enzymatic microenvironment
Topics:
Carbon quantum dotsEnzymesGrapheneNanocrystalsSemiconductor quantum dots
Cite this article:
Xin Q, Jia X, Nawaz A, et al. Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots. Nano Research, 2020, 13(5): 1427-1433. https://doi.org/10.1007/s12274-020-2678-z
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Abstract Full text Electronic supplementary material About this article

The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes. From the perspective of mimicking active site microenvironment at low cost, we herein report a novel histidine-functionalized graphene quantum dot (His-GQD)/hemin complex, which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far. Also, our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range. Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic. In addition, His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose. Our work provides an effective design of artificial enzymes for practical applications.


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Abstract
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Electronic supplementary material
About this article

Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots

Show Author's information Qi Xin1,§, Xinrui Jia1,2,§, Asmat Nawaz1,2, Wenjing Xie1, Litao Li3, Jian Ru Gong1,2( )
CAS Center of Excellence for Nanoscience, CAS Key Laboratory for Nanosystem & Hierarchical Fabrication, National Center for Nanoscience and Technology, 11 Beiyitiao Zhongguancun, Beijing 100190, China
University of Chinese Academy of Sciences, Beijing 100049, China
Department of Orthopaedics, the 309th Hospital of the PLA, Beijing 100091, China

§ Qi Xin and Xinrui Jia contributed equally to this work.

Abstract

The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes. From the perspective of mimicking active site microenvironment at low cost, we herein report a novel histidine-functionalized graphene quantum dot (His-GQD)/hemin complex, which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far. Also, our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range. Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic. In addition, His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose. Our work provides an effective design of artificial enzymes for practical applications.

Keywords: graphene quantum dot, nanoenzyme, heme active site, peroxidase mimetic, enzymatic microenvironment

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Publication history
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Acknowledgements

Publication history

Received: 16 November 2019
Revised: 05 January 2020
Accepted: 26 January 2020
Published: 20 February 2020
Issue date: May 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020

Acknowledgements

The authors acknowledge financial support for this work from the National Key R&D Program "nanotechnology" special focus (No. 2016YFA0201600), the National Natural Science Foundation of China (Nos. 21422303, 21573049, 21872043, and 81602643), Beijing Natural Science Foundation (No. 2142036), and the Knowledge Innovation Program, Youth Innovation Promotion Association, and Special Program of "One Belt One Road" of CAS. The authors thank Dr. Dexing Li for the technique support and many helpful discussions for the ITC testing.

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