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Nano Research 2020, 13(2): 467-476 https://doi.org/10.1007/s12274-020-2631-1
Research Article | Issue | Published: 18 January 2020

Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model

Show Author's Information Aurore Van de Walle1( ),  Alexandre Fromain1,  Anouchka Plan Sangnier1,2,  Alberto Curcio1,  Luc Lenglet3,  Laurence Motte2( ),  Yoann Lalatonne2,4( ),  Claire Wilhelm1( )
Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University of Paris, 75205, Paris Cedex 13, France
Inserm, U1148, Laboratory for Vascular Translational Science, Sorbonne Paris Nord, Sorbonne Paris Cité, F-93017 Bobigny, France
Normafin Sàrl, 8 rue Mathilde Girault, 92300 Levallois-Perret, France
Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris, F-93009 Bobigny, France
Keywords:
stem cells, magnetic nanoparticles, magnetometry, biodegradation, real-time in operando measures
Topics:
BiodegradationCytologyIron oxidesMagnetic sensorsMedical nanotechnologyNanoparticlesStem cellsTissue
Cite this article:
Van de Walle A, Fromain A, Sangnier AP, et al. Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model. Nano Research, 2020, 13(2): 467-476. https://doi.org/10.1007/s12274-020-2631-1
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Abstract Full text Electronic supplementary material About this article

The use of magnetic nanoparticles in nanomedicine keeps expanding and, for most applications, the nanoparticles are internalized in cells then left within, bringing the need for accurate, fast, and easy to handle methodologies to assess their behavior in the cellular environment. Herein, a benchtop-size magnetic sensor is introduced to provide real-time precise measurement of nanoparticle magnetism within living cells. The values obtained with the sensor, of cells loaded with different doses of magnetic nanoparticles, are first compared to conventional vibrating sample magnetometry (VSM), and a strong correlation remarkably validates the use of the magnetic sensor as magnetometer to determine the nanoparticle cellular uptake. The sensor is then used to monitor the progressive intracellular degradation of the nanoparticles, over days. Importantly, this real-time in situ measure is performed on a stem cell-spheroid tissue model and can run continuously on a same spheroid, with cells kept alive within. Besides, such continuous magnetic measurement of cell magnetism at the tissue scale does not impact either tissue formation, viability, or stem cell function, including differentiation and extracellular matrix production.


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Electronic supplementary material
About this article

Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model

Show Author's information Aurore Van de Walle1( ), Alexandre Fromain1, Anouchka Plan Sangnier1,2, Alberto Curcio1, Luc Lenglet3, Laurence Motte2( ), Yoann Lalatonne2,4( ), Claire Wilhelm1( )
Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University of Paris, 75205, Paris Cedex 13, France
Inserm, U1148, Laboratory for Vascular Translational Science, Sorbonne Paris Nord, Sorbonne Paris Cité, F-93017 Bobigny, France
Normafin Sàrl, 8 rue Mathilde Girault, 92300 Levallois-Perret, France
Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris, F-93009 Bobigny, France

Abstract

The use of magnetic nanoparticles in nanomedicine keeps expanding and, for most applications, the nanoparticles are internalized in cells then left within, bringing the need for accurate, fast, and easy to handle methodologies to assess their behavior in the cellular environment. Herein, a benchtop-size magnetic sensor is introduced to provide real-time precise measurement of nanoparticle magnetism within living cells. The values obtained with the sensor, of cells loaded with different doses of magnetic nanoparticles, are first compared to conventional vibrating sample magnetometry (VSM), and a strong correlation remarkably validates the use of the magnetic sensor as magnetometer to determine the nanoparticle cellular uptake. The sensor is then used to monitor the progressive intracellular degradation of the nanoparticles, over days. Importantly, this real-time in situ measure is performed on a stem cell-spheroid tissue model and can run continuously on a same spheroid, with cells kept alive within. Besides, such continuous magnetic measurement of cell magnetism at the tissue scale does not impact either tissue formation, viability, or stem cell function, including differentiation and extracellular matrix production.

Keywords: stem cells, magnetic nanoparticles, magnetometry, biodegradation, real-time in operando measures

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Publication history
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Acknowledgements

Publication history

Received: 21 November 2019
Revised: 19 December 2019
Accepted: 27 December 2019
Published: 18 January 2020
Issue date: February 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020

Acknowledgements

This work was supported by the European Research Council (ERC-2014-CoG project MaTissE #648779). The authors would like to acknowledge the CNanoMat physico-chemical characterizations platform of University Paris 13, and Nicolas Chevalier for his help in controlling the CO2 level in the home-made incubator associated to the magnetic sensor.

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