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Published:
03 January 2020
Carbon nanostructure morphology templates nanocomposites for phosphoproteomics
Anna L. Capriotti1(
),
Silvia Marchesan2(
)
),
Silvia Marchesan2(
)
Topics:
Carbon blackCarbon nanotubesGraphdiyneGrapheneMolecular biologyMorphologyNanocompositesOxidation
An erratum to this article is available online at https://doi.org/10.1007/s12274-020-2643-xCite this article:
Piovesana S, Iglesias D, Melle-Franco M, et al.
Carbon nanostructure morphology templates nanocomposites for phosphoproteomics.
Nano Research,
2020, 13(2): 380-388.
https://doi.org/10.1007/s12274-020-2620-4
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Protein and peptide phosphorylation regulate numerous pathological processes, however, their characterization is challenging due to their low abundance and transient nature. Therefore, nanomaterials are being developed to address this demanding task. In particular, carbon nanostructures are attracting interest as scaffolds for functional nanocomposites, yet only isolated studies exist on the topic, and little is known on the effect of nanocarbon morphology on templating nanocomposites. In this work, we compared oxidized carbon nanotubes, graphene oxide, oxidized carbon nanohorns and oxidized graphitized carbon black, as scaffolds for magnetized nanocomposites. The nanomaterials were extensively characterized with experimental and in silico techniques. Next, they were applied to phosphopeptide enrichment from cancer cell lysates for NanoHPLC-MS/MS, with selectivity as high as nearly 90% and several-thousand identification hits. Overall, new insights emerged for the understanding and the design of nanocomposites for phosphoproteomics.
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Carbon nanostructure morphology templates nanocomposites for phosphoproteomics
Anna L. Capriotti1(
), Silvia Marchesan2(
)
), Silvia Marchesan2(
)
Abstract
Protein and peptide phosphorylation regulate numerous pathological processes, however, their characterization is challenging due to their low abundance and transient nature. Therefore, nanomaterials are being developed to address this demanding task. In particular, carbon nanostructures are attracting interest as scaffolds for functional nanocomposites, yet only isolated studies exist on the topic, and little is known on the effect of nanocarbon morphology on templating nanocomposites. In this work, we compared oxidized carbon nanotubes, graphene oxide, oxidized carbon nanohorns and oxidized graphitized carbon black, as scaffolds for magnetized nanocomposites. The nanomaterials were extensively characterized with experimental and in silico techniques. Next, they were applied to phosphopeptide enrichment from cancer cell lysates for NanoHPLC-MS/MS, with selectivity as high as nearly 90% and several-thousand identification hits. Overall, new insights emerged for the understanding and the design of nanocomposites for phosphoproteomics.
Keywords:
nanocomposites, carbon nanotubes, graphene oxide, magnetic, carbon nanohorns, proteomics
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Publication history
Copyright
Acknowledgements
Publication history
Received: 30 September 2019
Revised: 15 December 2019
Accepted: 21 December 2019
Published:
03 January 2020
Issue date: February 2020
Copyright
© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Acknowledgements
This work was supported by PRIN 2015, project number 2015TWP83Z. The authors would like to acknowledge networking support by the COST Action MultiComp CA15107, as well as financial support from the Slovenian Research Agency (ARRS) for research core funding No. (P2-0089) and projects No. (J1-7302 and J3-7494).
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