Metal-organic frameworks nanoswitch: Toward photo-controllable endo/lysosomal rupture and release for enhanced cancer RNA interference

Gan Lin; Yang Zhang; Long Zhang; Junqing Wang; Ye Tian; Wen Cai; Shangui Tang; Chengchao Chu; JiaJing Zhou; Peng Mi; Xiaoyuan Chen; Gang Liu

doi:10.1007/s12274-019-2606-2

Nano Research 2020, 13(1): 238-245 https://doi.org/10.1007/s12274-019-2606-2

Research Article | Issue | Published: 03 January 2020

Metal-organic frameworks nanoswitch: Toward photo-controllable endo/lysosomal rupture and release for enhanced cancer RNA interference

Show Author's Information Hide Author's Information Gan Lin^{¹^,²^,^§}, Yang Zhang^{¹^,^§}, Long Zhang^{¹^,^§}, Junqing Wang^¹, Ye Tian^¹, Wen Cai^¹, Shangui Tang^¹, Chengchao Chu^¹, JiaJing Zhou^³, Peng Mi^⁴, Xiaoyuan Chen^⁵, Gang Liu^¹(

)

1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China

2The Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia

3School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore

4State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China

5Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA

^§ Gan Lin, Yang Zhang, and Long Zhang contributed equally to this work.

Keywords:

metal-organic frameworks, cancer therapy, endo/lysosomal rupture and release, photo-controllable nanoswitch, RNA interference

Topics:

Diseases Gene transfer Genes Oncology Organometallics RNA Targeted drug delivery

Cite this article:

Lin G, Zhang Y, Zhang L, et al. Metal-organic frameworks nanoswitch: Toward photo-controllable endo/lysosomal rupture and release for enhanced cancer RNA interference. Nano Research, 2020, 13(1): 238-245. https://doi.org/10.1007/s12274-019-2606-2

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Abstract Full text Electronic supplementary material About this article

Abstract

Endo/lysosomal escape and gene release are two critical bottlenecks in gene delivery. Herein, a novel photo-controllable metal-organic frameworks (MOFs) nanoswitch is rationally designed for enhancing small interfering RNA (siRNA) delivery. One single laser triggers the "off-to-on" switching of MOFs nanocomplexes, inducing significant siRNA release accompanied by rapid MOFs dissociation into protonatable 2-methylimidazalo and osmotic rupturing Zn²⁺ ions, which cooperatively contribute to remarkable endo/lysosomal rupture (~ 90%). The simultaneous endo/lysosomal rupture and release enable a high spatio-temporal control on RNA interference for effective cancer therapy. Notably, the "off-to-on" switching also activates fluorescence recovery for real-time monitoring siRNA delivery. The nanoswitch could easily be extended to deliver other therapeutic agents (e.g., DNA, protein, anticancer drug) for overcoming endo/lysosomal entrapment.

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About this article

Metal-organic frameworks nanoswitch: Toward photo-controllable endo/lysosomal rupture and release for enhanced cancer RNA interference

Show Author's information Hide Author's Information Gan Lin^{¹^,²^,^§}, Yang Zhang^{¹^,^§}, Long Zhang^{¹^,^§}, Junqing Wang^¹, Ye Tian^¹, Wen Cai^¹, Shangui Tang^¹, Chengchao Chu^¹, JiaJing Zhou^³, Peng Mi^⁴, Xiaoyuan Chen^⁵, Gang Liu^¹(

)

1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China

2The Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia

3School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore

4State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China

5Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA

^§ Gan Lin, Yang Zhang, and Long Zhang contributed equally to this work.

Abstract

Keywords: metal-organic frameworks, cancer therapy, endo/lysosomal rupture and release, photo-controllable nanoswitch, RNA interference

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Acknowledgements

Publication history

Received: 09 September 2019

Revised: 09 December 2019

Accepted: 15 December 2019

Published: 03 January 2020

Issue date: January 2020

Copyright

Acknowledgements

We acknowledge Jingru Huang and Baoying Xie from Central Laboratory in School of Medicine, Xiamen University for assistance with inductively coupled plasma experiment, laser scanning confocal microscope and data analysis. This work was supported by the Major State Basic Research Development Program of China (No. 2017YFA0205201), the National Natural Science Foundation of China (Nos. 81925019, 81422023, U1705281, and U1505221), the Fundamental Research Funds for the Central Universities (No. 20720190088), and the Program for New Century Excellent Talents in University, China (No. NCET-13-0502). All animal experiments were approved by the Animal Management and Ethics Committee of the Xiamen University.