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Nano Research 2020, 13(1): 203-208 https://doi.org/10.1007/s12274-019-2598-y
Research Article | Issue | Published: 02 January 2020

Embedding ultrasmall Ag nanoclusters in Luria-Bertani extract via light irradiation for enhanced antibacterial activity

Show Author's Information Ziping Wang1,2,  Yushuang Fang1,  Xianfeng Zhou3,  Zhibo Li3,  Haiguang Zhu1,  Fanglin Du1,  Xun Yuan1 ( ),  Qiaofeng Yao4( ),  Jianping Xie4( )
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Weifang University of Science and Technology, Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang 262700, China
Key Lab of Biobased Polymer Materials of Shandong Provincial Education Department, School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore
Keywords:
reactive oxygen species, Ag nanoclusters, Luria-Bertani (LB) broth, light irradiation, antibacterial agent
Topics:
Bacteria BactericidesBiocompatibilityIrradiationNanoclusters
Cite this article:
Wang Z, Fang Y, Zhou X, et al. Embedding ultrasmall Ag nanoclusters in Luria-Bertani extract via light irradiation for enhanced antibacterial activity. Nano Research, 2020, 13(1): 203-208. https://doi.org/10.1007/s12274-019-2598-y
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Abstract Full text Electronic supplementary material About this article

Ultrasmall silver nanoclusters (Ag NCs) with rich surface chemistry and good biocompatibility are promising in antibacterial application, however, further development of Ag NCs for practical settings has been constrained by their relatively weak antibacterial activity. Using the nutritionally-rich medium for bacteria (e.g., Luria-Bertani (LB) medium) to coat active Ag NCs could further improve their antibacterial activity. Here, we provide a delicate design of a highly efficient Ag NCs@ELB antibacterial agent (ELB denotes the extract of LB medium) by anchoring Ag NCs inside the ELB species via light irradiation. The as-designed Ag NCs with bacterium-favored nutrients on the surface can be easily swallowed by the bacteria, boosting the production of the intracellular reactive oxygen species (ROS, about 2-fold of that in the pristine Ag NCs). Subsequently, a higher concentration of ROS generated in Ag NCs@ELB leads to enhanced antibacterial activity, and enables to reduce the colony forming units (CFU) of both gram-positive and gram-negative bacteria with 3-4 orders of magnitude less than that treated with the pristine Ag NCs. In addition, the Ag NCs@ELB also shows good biocompatibility. This study suggests that surface engineering of active species (e.g., Ag NCs) with nutritionally-rich medium of the bacteria is an efficient way to improve their antibacterial activity.


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Electronic supplementary material
About this article

Embedding ultrasmall Ag nanoclusters in Luria-Bertani extract via light irradiation for enhanced antibacterial activity

Show Author's information Ziping Wang1,2, Yushuang Fang1, Xianfeng Zhou3, Zhibo Li3, Haiguang Zhu1, Fanglin Du1, Xun Yuan1 ( ), Qiaofeng Yao4( ), Jianping Xie4( )
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Weifang University of Science and Technology, Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang 262700, China
Key Lab of Biobased Polymer Materials of Shandong Provincial Education Department, School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore

Abstract

Ultrasmall silver nanoclusters (Ag NCs) with rich surface chemistry and good biocompatibility are promising in antibacterial application, however, further development of Ag NCs for practical settings has been constrained by their relatively weak antibacterial activity. Using the nutritionally-rich medium for bacteria (e.g., Luria-Bertani (LB) medium) to coat active Ag NCs could further improve their antibacterial activity. Here, we provide a delicate design of a highly efficient Ag NCs@ELB antibacterial agent (ELB denotes the extract of LB medium) by anchoring Ag NCs inside the ELB species via light irradiation. The as-designed Ag NCs with bacterium-favored nutrients on the surface can be easily swallowed by the bacteria, boosting the production of the intracellular reactive oxygen species (ROS, about 2-fold of that in the pristine Ag NCs). Subsequently, a higher concentration of ROS generated in Ag NCs@ELB leads to enhanced antibacterial activity, and enables to reduce the colony forming units (CFU) of both gram-positive and gram-negative bacteria with 3-4 orders of magnitude less than that treated with the pristine Ag NCs. In addition, the Ag NCs@ELB also shows good biocompatibility. This study suggests that surface engineering of active species (e.g., Ag NCs) with nutritionally-rich medium of the bacteria is an efficient way to improve their antibacterial activity.

Keywords: reactive oxygen species, Ag nanoclusters, Luria-Bertani (LB) broth, light irradiation, antibacterial agent

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Publication history
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Acknowledgements

Publication history

Received: 20 October 2019
Revised: 01 December 2019
Accepted: 05 December 2019
Published: 02 January 2020
Issue date: January 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Acknowledgements

This work is supported by the Taishan Scholar Foundation (No. tsqn201812074), the Young Talents Joint Fund of Shandong Province (No. ZR2019YQ07), the Original Innovation Project of Qingdao City (No. 18-2-2-58-jch), the Open Fund of Shandong Key Laboratory of Biochemical Analysis (No. QUSTHX201901), and the Ministry of Education, Singapore, Academic Research Grant R-279-000-538-114.

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