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Nano Research 2019, 12(12): 3037-3043 https://doi.org/10.1007/s12274-019-2549-7
Research Article | Issue | Published: 31 October 2019

Radioiodinated tyrosine based carbon dots with efficient renal clearance for single photon emission computed tomography of tumor

Show Author's Information Nian Liu1,2,3,§,  Yiyue Shi1,§,  Jingru Guo1,  Hai Li4,  Qiang Wang5,  Menglin Song3,  Zhiyuan Shi3,  Le He4,  Xinhui Su5,  Jin Xie6,  Xiaolian Sun1( )
State Key Laboratory of Natural MedicinesKey Laboratory of Drug Quality Control and PharmacovigilanceDepartment of Pharmaceutical AnalysisChina Pharmaceutical UniversityNanjing210009China
Institute of Biological and Medical ImagingHelmholtz Zentrum München and Technische Universit?t MünchenNeuherberg85764Germany
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational MedicineSchool of Public HealthXiamen UniversityXiamen361005China
Institute of Functional Nano & Soft Materials (FUNSOM)Jiangsu Key Laboratory for Carbon-Based Functional Materials & DevicesSoochow UniversitySuzhou215123China
Department of Nuclear MedicineZhongshan Hospital Xiamen UniversityXiamen361004China
Department of ChemistryBio-Imaging Research CenterUniversity of GeorgiaAthensGeorgia30602USA

§Nian Liu and Yiyue Shi contributed equally to this work.

Keywords:
renal clearance, radioiodination, tyrosine based carbon dots, single photon emission computed tomography (SPECT) imaging, fluoresecent imaging
Topics:
BiocompatibilityCarbonDiagnosisFluorescence imagingMammalsMedical applicationsOptical propertiesPhotonsTumors
Cite this article:
Liu N, Shi Y, Guo J, et al. Radioiodinated tyrosine based carbon dots with efficient renal clearance for single photon emission computed tomography of tumor. Nano Research, 2019, 12(12): 3037-3043. https://doi.org/10.1007/s12274-019-2549-7
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Abstract Full text Electronic supplementary material About this article

Nanoparticles with effective tumor accumulation and efficient renal clearance have attracted significant interests for clinical applications. We prepared 2.5 nm tyrosine based carbon dots (TCDs) with phenolic hydroxyl groups on the surface for directly 125I labeling. The 125I labeled polyethylene glycol (PEG) functionalized TCDs (125I-TCDPEGs) showed excellent radiochemical stability both in vitro and in vivo. Due to the enhanced permeability and retention effect, these 125I-TCDPEGs demonstrated a tumor accumulation around 4%-5% of the injected dose per gram (ID/g) for U87MG, 4T1, HepG2 and MCF7 tumor-bearing mice at 1 h post-injection. Meanwhile, the 125I-TCDPEGs also could be fast renally excreted, with less than 0.6% ID/g left in the liver and spleen within 24 h. These radioactive carbon dots not only can be used for cellular fluorescence imaging due to their intrinsic optical property, but are also effective single photon emission computed tomography (SPECT) imaging agents for tumor. Together with their excellent biocompatibility and stability, we anticipate these 125I-TCDPEGs of great potential for early tumor diagnosis in clinic. What's more, our TCDPEGs are also proved to be feasible carriers for other iodine isotopes such as 127I and 131I for different biomedical application.


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Abstract
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Electronic supplementary material
About this article

Radioiodinated tyrosine based carbon dots with efficient renal clearance for single photon emission computed tomography of tumor

Show Author's information Nian Liu1,2,3,§, Yiyue Shi1,§, Jingru Guo1, Hai Li4, Qiang Wang5, Menglin Song3, Zhiyuan Shi3, Le He4, Xinhui Su5, Jin Xie6, Xiaolian Sun1( )
State Key Laboratory of Natural MedicinesKey Laboratory of Drug Quality Control and PharmacovigilanceDepartment of Pharmaceutical AnalysisChina Pharmaceutical UniversityNanjing210009China
Institute of Biological and Medical ImagingHelmholtz Zentrum München and Technische Universit?t MünchenNeuherberg85764Germany
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational MedicineSchool of Public HealthXiamen UniversityXiamen361005China
Institute of Functional Nano & Soft Materials (FUNSOM)Jiangsu Key Laboratory for Carbon-Based Functional Materials & DevicesSoochow UniversitySuzhou215123China
Department of Nuclear MedicineZhongshan Hospital Xiamen UniversityXiamen361004China
Department of ChemistryBio-Imaging Research CenterUniversity of GeorgiaAthensGeorgia30602USA

§Nian Liu and Yiyue Shi contributed equally to this work.

Abstract

Nanoparticles with effective tumor accumulation and efficient renal clearance have attracted significant interests for clinical applications. We prepared 2.5 nm tyrosine based carbon dots (TCDs) with phenolic hydroxyl groups on the surface for directly 125I labeling. The 125I labeled polyethylene glycol (PEG) functionalized TCDs (125I-TCDPEGs) showed excellent radiochemical stability both in vitro and in vivo. Due to the enhanced permeability and retention effect, these 125I-TCDPEGs demonstrated a tumor accumulation around 4%-5% of the injected dose per gram (ID/g) for U87MG, 4T1, HepG2 and MCF7 tumor-bearing mice at 1 h post-injection. Meanwhile, the 125I-TCDPEGs also could be fast renally excreted, with less than 0.6% ID/g left in the liver and spleen within 24 h. These radioactive carbon dots not only can be used for cellular fluorescence imaging due to their intrinsic optical property, but are also effective single photon emission computed tomography (SPECT) imaging agents for tumor. Together with their excellent biocompatibility and stability, we anticipate these 125I-TCDPEGs of great potential for early tumor diagnosis in clinic. What's more, our TCDPEGs are also proved to be feasible carriers for other iodine isotopes such as 127I and 131I for different biomedical application.

Keywords: renal clearance, radioiodination, tyrosine based carbon dots, single photon emission computed tomography (SPECT) imaging, fluoresecent imaging

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Publication history
Copyright
Acknowledgements

Publication history

Received: 20 September 2019
Revised: 18 October 2019
Accepted: 21 October 2019
Published: 31 October 2019
Issue date: December 2019

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Acknowledgements

Acknowledgements

X. S. acknowledges the National Key Research and Development Program of China (No. 2016YFA0203600), the National Natural Science Foundation of China (Nos. 81971738 and 81571743), the Project Program of State Key Laboratory of Natural Medicines, and the China Pharmaceutical University (No. SKLNMZZRC05).

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