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Nano Research 2019, 12(12): 3024-3030 https://doi.org/10.1007/s12274-019-2546-x
Research Article | Issue | Published: 31 October 2019

A novel chitosan nanocapsule for enhanced skin penetration of cyclosporin A and effective hair growth in vivo

Show Author's Information Jin Sil Lee1,2,§,  Youngmin Hwang2,  Hyeryeon Oh1,2,§,  Sunghyun Kim1,  Jin-Hwa Kim3,  Jeung-Hoon Lee3,  Yong Chul Shin3,4,  Giyoong Tae2( ),  Won Il Choi1( )
Center for Convergence Bioceramic MaterialsConvergence R&D DivisionKorea Institute of Ceramic Engineering and Technology202Osongsaengmyeong 1-roOsong-eupHeungdeok-guCheongjuChungbuk202Republic of Korea
School of Materials Science and EngineeringGwangju Institute of Science and Technology123 Cheomdan-gwagiroBuk-guGwangju61005Republic of Korea
SKINMED Co.Ltd.Daejeon34028Republic of Korea
AmicogenInc.64 Dongburo 1259JinsungJinju52621Republic of Korea

§Jin Sil Lee and Youngmin Hwang contributed equally to this work.

Keywords:
chitosan, nanocapsule, cyclosporin A, transdermal delivery, hair growth
Topics:
Chemotherapy Controlled drug deliveryMammalsMetabolitesNanocapsules Targeted drug delivery
Cite this article:
Lee JS, Hwang Y, Oh H, et al. A novel chitosan nanocapsule for enhanced skin penetration of cyclosporin A and effective hair growth in vivo. Nano Research, 2019, 12(12): 3024-3030. https://doi.org/10.1007/s12274-019-2546-x
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Abstract Full text Electronic supplementary material About this article

Hair loss due to medical conditions, such as alopecia, male pattern baldness, and cancer chemotherapy treatment, has been a common problem for many individuals. Cyclosporin A (CsA), a fungal metabolite, has been reported to be a hair growth modulatory agent and is a potential drug for hair regeneration. However, the effect of topical application of CsA is limited by its poor water solubility. Several delivery systems developed to enhance its solubility still showed poor skin penetration. To overcome these limitations, in this study, we have developed a novel chitosan nanocapsule platform using Pluronic F127 and chitosan without any chemical crosslinking or complicated preparation steps for the enhanced water solubility and high transdermal penetration of CsA. The chitosan nanocapsules (ChiNCs) optimized in terms of structural stability by using chitosan with various molecular weights ranging from 3 to 100 kDa enhanced the skin permeation of CsA through human cadaver skin in vitro. Topical administration of the CsA loaded ChiNCs increased the hair follicles by c.a. 7 times higher than that of the control group, and effectively induced hair growth in C57BL/6 mice in vivo. These results suggest that ChiNCs could be used as a platform for effective transdermal delivery of various hydrophobic drugs.


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Abstract
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Electronic supplementary material
About this article

A novel chitosan nanocapsule for enhanced skin penetration of cyclosporin A and effective hair growth in vivo

Show Author's information Jin Sil Lee1,2,§, Youngmin Hwang2, Hyeryeon Oh1,2,§, Sunghyun Kim1, Jin-Hwa Kim3, Jeung-Hoon Lee3, Yong Chul Shin3,4, Giyoong Tae2( ), Won Il Choi1( )
Center for Convergence Bioceramic MaterialsConvergence R&D DivisionKorea Institute of Ceramic Engineering and Technology202Osongsaengmyeong 1-roOsong-eupHeungdeok-guCheongjuChungbuk202Republic of Korea
School of Materials Science and EngineeringGwangju Institute of Science and Technology123 Cheomdan-gwagiroBuk-guGwangju61005Republic of Korea
SKINMED Co.Ltd.Daejeon34028Republic of Korea
AmicogenInc.64 Dongburo 1259JinsungJinju52621Republic of Korea

§Jin Sil Lee and Youngmin Hwang contributed equally to this work.

Abstract

Hair loss due to medical conditions, such as alopecia, male pattern baldness, and cancer chemotherapy treatment, has been a common problem for many individuals. Cyclosporin A (CsA), a fungal metabolite, has been reported to be a hair growth modulatory agent and is a potential drug for hair regeneration. However, the effect of topical application of CsA is limited by its poor water solubility. Several delivery systems developed to enhance its solubility still showed poor skin penetration. To overcome these limitations, in this study, we have developed a novel chitosan nanocapsule platform using Pluronic F127 and chitosan without any chemical crosslinking or complicated preparation steps for the enhanced water solubility and high transdermal penetration of CsA. The chitosan nanocapsules (ChiNCs) optimized in terms of structural stability by using chitosan with various molecular weights ranging from 3 to 100 kDa enhanced the skin permeation of CsA through human cadaver skin in vitro. Topical administration of the CsA loaded ChiNCs increased the hair follicles by c.a. 7 times higher than that of the control group, and effectively induced hair growth in C57BL/6 mice in vivo. These results suggest that ChiNCs could be used as a platform for effective transdermal delivery of various hydrophobic drugs.

Keywords: chitosan, nanocapsule, cyclosporin A, transdermal delivery, hair growth

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Publication history
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Acknowledgements

Publication history

Received: 08 September 2019
Revised: 15 October 2019
Accepted: 16 October 2019
Published: 31 October 2019
Issue date: December 2019

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Acknowledgements

Acknowledgements

This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) (Nos. NRF-2018R1D1A1B07043620 and 2018R1A4A1024963) and the grant of Korea Institute of Ceramic Engineering and Technology (KICET).

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