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Nano Research 2020, 13(5): 1318-1323 https://doi.org/10.1007/s12274-019-2534-1
Research Article | Issue | Published: 23 October 2019

3D printing of living bacteria electrode

Show Author's Information Megan C. Freyman§,  Tianyi Kou§,  Shanwen Wang,  Yat Li( )
Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA

§Megan C. Freyman and Tianyi Kou contributed equally to this work.

Keywords:
three-dimensional (3D) printing, Shewanella Oneidensis MR-1, microbial fuel cells, bacteria ink, living electrode
Topics:
AnodesBacteria Carbon blackMicrobial fuel cells
Cite this article:
Freyman MC, Kou T, Wang S, et al. 3D printing of living bacteria electrode. Nano Research, 2020, 13(5): 1318-1323. https://doi.org/10.1007/s12274-019-2534-1
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Abstract Full text Electronic supplementary material About this article

The capability of preparing three-dimensional (3D) printable living inks provides a unique way to harness the activity of microbes and use them in functional devices. Here we demonstrate the incorporation of the living bacteria Shewanella Oneidensis MR-1 (S. Oneidensis MR-1) directly into an ink used for creating 3D printed structures. Significantly, S. Oneidensis MR-1 survives the 3D printing process by showing prominent activity in degrading the methyl orange azo dye. Through the addition of carbon black to this ink, we further demonstrate the direct printing of a living microbial fuel cell (MFC) anode. To our knowledge, this is the first report on implementing 3D printed bacteria structure as a living electrode for an MFC system. The capability of printing living and functional 3D bacterial structure could open up new possibilities in design and fabrication of microbial devices as well as fundamental research on the interaction between different bacterial strains, electrode materials, and surrounding environments.


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Abstract
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Electronic supplementary material
About this article

3D printing of living bacteria electrode

Show Author's information Megan C. Freyman§, Tianyi Kou§, Shanwen Wang, Yat Li( )
Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA

§Megan C. Freyman and Tianyi Kou contributed equally to this work.

Abstract

The capability of preparing three-dimensional (3D) printable living inks provides a unique way to harness the activity of microbes and use them in functional devices. Here we demonstrate the incorporation of the living bacteria Shewanella Oneidensis MR-1 (S. Oneidensis MR-1) directly into an ink used for creating 3D printed structures. Significantly, S. Oneidensis MR-1 survives the 3D printing process by showing prominent activity in degrading the methyl orange azo dye. Through the addition of carbon black to this ink, we further demonstrate the direct printing of a living microbial fuel cell (MFC) anode. To our knowledge, this is the first report on implementing 3D printed bacteria structure as a living electrode for an MFC system. The capability of printing living and functional 3D bacterial structure could open up new possibilities in design and fabrication of microbial devices as well as fundamental research on the interaction between different bacterial strains, electrode materials, and surrounding environments.

Keywords: three-dimensional (3D) printing, Shewanella Oneidensis MR-1, microbial fuel cells, bacteria ink, living electrode

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Publication history
Copyright
Acknowledgements

Publication history

Received: 29 August 2019
Revised: 03 October 2019
Accepted: 04 October 2019
Published: 23 October 2019
Issue date: May 2020

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Acknowledgements

M. F. and T. K. contributed equally to this work. We acknowledge Dr. Tom Yuzvinsky from the W. M. Keck Center for Nanoscale Optofluidics, University of California, Santa Cruz (UCSC) for SEM image acquisition. The authors also thank Prof. Jin Zhang at UCSC for allowing the use of their UV-vis spectrometer, and Dr. Ben Abrams at Institute for the Biology of Stem Cells at UCSC for taking confocal microscopy images.

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