Prussian blue with intrinsic heme-like structure as peroxidase mimic
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Despite the intrinsic peroxidase-like activity of Prussian blue nanoparticles (PBNPs), their enzyme-mimic mechanism has been scarcely investigated to date. Herein, we probed the catalytic site of PBNPs for the first time, by comparing their peroxidase-like activity with that of a series of Prussian blue analogs (PBAs) in which Fe atoms were replaced by Co, Ni, and Cu. The PBNPs exhibited the highest maximal reaction velocity (1.941 μM·s-1), which was at least 13 times higher than that of the PBAs, demonstrating that the peroxidase-like properties of PBNPs could be ascribed to the FeNx (x = 4-6) instead of the FeC6 units. Notably, the PBNPs/H2O2 couple also showed much higher oxidizability than ·OH radicals produced from the Fenton reaction, implying that a high active Fe(Ⅳ)=O intermediate might be formed in the FeNx units. This study can thus pave the way for the wider application of PBNPs in biomimetic reactions.
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Prussian blue with intrinsic heme-like structure as peroxidase mimic
)
Abstract
Despite the intrinsic peroxidase-like activity of Prussian blue nanoparticles (PBNPs), their enzyme-mimic mechanism has been scarcely investigated to date. Herein, we probed the catalytic site of PBNPs for the first time, by comparing their peroxidase-like activity with that of a series of Prussian blue analogs (PBAs) in which Fe atoms were replaced by Co, Ni, and Cu. The PBNPs exhibited the highest maximal reaction velocity (1.941 μM·s-1), which was at least 13 times higher than that of the PBAs, demonstrating that the peroxidase-like properties of PBNPs could be ascribed to the FeNx (x = 4-6) instead of the FeC6 units. Notably, the PBNPs/H2O2 couple also showed much higher oxidizability than ·OH radicals produced from the Fenton reaction, implying that a high active Fe(Ⅳ)=O intermediate might be formed in the FeNx units. This study can thus pave the way for the wider application of PBNPs in biomimetic reactions.
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Acknowledgements
We are grateful for the support from the National Natural Science Foundation of China (Nos. 21375123 and 21675151) and the Ministry of Science and Technology of China (Nos. 2013YQ170585 and 2016YFA0203203).
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