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Nano Research 2018, 11(8): 4380-4389 https://doi.org/10.1007/s12274-018-2027-7
Research Article | Issue | Published: 09 March 2018

Enzyme@silica nanoflower@metal-organic framework hybrids: A novel type of integrated nanobiocatalysts with improved stability

Show Author's Information Yingjie Du1,  Jing Gao1,2,  Huajiao Liu1,  Liya Zhou1,  Li Ma1,  Ying He1,  Zhihong Huang1,  Yanjun Jiang1,2( )
School of Chemical Engineering and TechnologyHebei University of Technology8 Guangrong RoadHongqiao DistrictTianjin300130China
Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy SavingHebei University of Technology8 Guangrong RoadHongqiao DistrictTianjin300130China
Keywords:
hybrids, immobilization, metal-organic framework, silica nanoflower, nanobiocatalyst
Cite this article:
Du Y, Gao J, Liu H, et al. Enzyme@silica nanoflower@metal-organic framework hybrids: A novel type of integrated nanobiocatalysts with improved stability. Nano Research, 2018, 11(8): 4380-4389. https://doi.org/10.1007/s12274-018-2027-7
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Abstract Full text Electronic supplementary material About this article

A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework (enzyme@SNF@ZIF-8) structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase (PGA) and catalase (CAT) as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8(2) structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8(2) shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers (PGA@SNF). The obtained CAT-based nanobiocatalysts (CAT@SNF@ZIF-8(2)) also show excellent catalytic performance.


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Electronic supplementary material
About this article

Enzyme@silica nanoflower@metal-organic framework hybrids: A novel type of integrated nanobiocatalysts with improved stability

Show Author's information Yingjie Du1, Jing Gao1,2, Huajiao Liu1, Liya Zhou1, Li Ma1, Ying He1, Zhihong Huang1, Yanjun Jiang1,2( )
School of Chemical Engineering and TechnologyHebei University of Technology8 Guangrong RoadHongqiao DistrictTianjin300130China
Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy SavingHebei University of Technology8 Guangrong RoadHongqiao DistrictTianjin300130China

Abstract

A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework (enzyme@SNF@ZIF-8) structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase (PGA) and catalase (CAT) as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8(2) structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8(2) shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers (PGA@SNF). The obtained CAT-based nanobiocatalysts (CAT@SNF@ZIF-8(2)) also show excellent catalytic performance.

Keywords: hybrids, immobilization, metal-organic framework, silica nanoflower, nanobiocatalyst

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Publication history
Copyright
Acknowledgements

Publication history

Received: 13 January 2018
Revised: 10 February 2018
Accepted: 13 February 2018
Published: 09 March 2018
Issue date: August 2018

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Acknowledgements

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21576068, 21276060, and 21276062), the Natural Science Foundation of Tianjin City (No. 16JCYBJC19800), the Natural Science Foundation of Hebei Province (Nos. B2015202082, B2016202027, and B2017202056), the Program for Top 100 Innovative Talents in Colleges and Universities of Hebei Province (No. SLRC2017029), Hebei High Level Personnel of Support Program (No. A2016002027), and the Graduate Innovation Support Program in Hebei Province (No. CXZZSS2017024).

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