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Nano Research 2018, 11(8): 4005-4016 https://doi.org/10.1007/s12274-018-1982-3
Research Article | Issue | Published: 23 January 2018

Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model

Show Author's Information Hsin-Neng Wang1,2,§,  Janna K. Register1,2,§,  Andrew M. Fales1,2,§,  Naveen Gandra1,2,  Eugenia H. Cho3,  Alina Boico3,  Gregory M. Palmer3,  Bruce Klitzman3,  Tuan Vo-Dinh1,2,4( )
Departments of Biomedical EngineeringDuke UniversityDurhamNC27708USA
Fitzpatrick Institute for PhotonicsDuke UniversityDurhamNC27708USA
Medical CenterDuke UniversityDurhamNC27710USA
Departments of ChemistryDuke UniversityDurhamNC27708USA

§ Hsin-Neng Wang, Janna K. Register and Andrew M. Fales contributed equally to this work.

Keywords:
plasmonics, surface-enhanced Raman scattering (SERS), nanosensor, nanoprobes, nanostar, in vivo sensing
Cite this article:
Wang H-N, Register JK, Fales AM, et al. Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model. Nano Research, 2018, 11(8): 4005-4016. https://doi.org/10.1007/s12274-018-1982-3
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Abstract Full text About this article

Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the "inverse molecular sentinel" detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the "tissue optical window", rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core–shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing.


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About this article

Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model

Show Author's information Hsin-Neng Wang1,2,§, Janna K. Register1,2,§, Andrew M. Fales1,2,§, Naveen Gandra1,2, Eugenia H. Cho3, Alina Boico3, Gregory M. Palmer3, Bruce Klitzman3, Tuan Vo-Dinh1,2,4( )
Departments of Biomedical EngineeringDuke UniversityDurhamNC27708USA
Fitzpatrick Institute for PhotonicsDuke UniversityDurhamNC27708USA
Medical CenterDuke UniversityDurhamNC27710USA
Departments of ChemistryDuke UniversityDurhamNC27708USA

§ Hsin-Neng Wang, Janna K. Register and Andrew M. Fales contributed equally to this work.

Abstract

Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the "inverse molecular sentinel" detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the "tissue optical window", rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core–shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing.

Keywords: plasmonics, surface-enhanced Raman scattering (SERS), nanosensor, nanoprobes, nanostar, in vivo sensing

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Publication history
Copyright
Acknowledgements

Publication history

Received: 20 April 2017
Revised: 03 January 2018
Accepted: 05 January 2018
Published: 23 January 2018
Issue date: August 2018

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Acknowledgements

Acknowledgements

This work was sponsored by the Defense Advanced Research Projects Agency (No. HR0011-13-2-0003). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

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