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Nano Research 2018, 11(4): 2012-2023 https://doi.org/10.1007/s12274-017-1817-7
Research Article | Issue | Published: 19 March 2018

Novel dual fluorescence temperature-sensitive chameleon DNA-templated silver nanocluster pair for intracellular thermometry

Show Author's Information Weijun Zhou1,2,  Jinbo Zhu1,  Ye Teng1,  Baoji Du1,2,  Xu Han1,  Shaojun Dong1,2( )
State Key Laboratory of Electroanalytical ChemistryChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China
University of Chinese Academy of SciencesBeijing100049China
Keywords:
fluorescence, DNA-templated silver nanocluster (AgNC), chameleon, temperature-sensitive
Cite this article:
Zhou W, Zhu J, Teng Y, et al. Novel dual fluorescence temperature-sensitive chameleon DNA-templated silver nanocluster pair for intracellular thermometry. Nano Research, 2018, 11(4): 2012-2023. https://doi.org/10.1007/s12274-017-1817-7
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Abstract Full text Electronic supplementary material About this article

For the first time, we are reporting a novel type of dual fluorescence temperature-sensitive DNA-templated silver nanocluster (AgNC) pair, which contains two pieces of single-stranded AgNC in proximity through hybridization. Both the chameleon AgNC pairs, A-NCP and B-NCP, possess two bright fluorescence peaks that achieve sensitive variations corresponding to temperature change from 15 to 45 ℃. With the increase in temperature, one of the fluorescence emissions of A-NCP (A-FL570) increases, while the other (A-FL640) decreases. However, both the emissions of B-NCP (B-FL685 and B-FL620) decrease simultaneously. Therefore, A-NCP shows a remarkable fluorescence color variation from orange to yellow, while the fluorescence color of B-NCP changes from orange to colorless, with increase in temperature. Moreover, the temperature responding linear range of A-NCP can be regulated by adjusting the structures and sequences of assistant DNA templates. It is assumed that the two single-stranded segmental AgNCs are integrated together as they are assembled into AgNC pairs, leading to a dramatic variation in fluorescence properties. The temperature-sensitive phenomenon is due to the dehybridization-induced separation of two pieces of segmental AgNC, caused by temperature increase. The temperature-sensitive AgNC pairs have been successful in indicating the temperature of living cells, showing the potential for a new application of silver nanocluster as a nanothermometer with adjustable response range, bringing novel insight into the regulatory mechanism of AgNC fluorescence variation.


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Electronic supplementary material
About this article

Novel dual fluorescence temperature-sensitive chameleon DNA-templated silver nanocluster pair for intracellular thermometry

Show Author's information Weijun Zhou1,2, Jinbo Zhu1, Ye Teng1, Baoji Du1,2, Xu Han1, Shaojun Dong1,2( )
State Key Laboratory of Electroanalytical ChemistryChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China
University of Chinese Academy of SciencesBeijing100049China

Abstract

For the first time, we are reporting a novel type of dual fluorescence temperature-sensitive DNA-templated silver nanocluster (AgNC) pair, which contains two pieces of single-stranded AgNC in proximity through hybridization. Both the chameleon AgNC pairs, A-NCP and B-NCP, possess two bright fluorescence peaks that achieve sensitive variations corresponding to temperature change from 15 to 45 ℃. With the increase in temperature, one of the fluorescence emissions of A-NCP (A-FL570) increases, while the other (A-FL640) decreases. However, both the emissions of B-NCP (B-FL685 and B-FL620) decrease simultaneously. Therefore, A-NCP shows a remarkable fluorescence color variation from orange to yellow, while the fluorescence color of B-NCP changes from orange to colorless, with increase in temperature. Moreover, the temperature responding linear range of A-NCP can be regulated by adjusting the structures and sequences of assistant DNA templates. It is assumed that the two single-stranded segmental AgNCs are integrated together as they are assembled into AgNC pairs, leading to a dramatic variation in fluorescence properties. The temperature-sensitive phenomenon is due to the dehybridization-induced separation of two pieces of segmental AgNC, caused by temperature increase. The temperature-sensitive AgNC pairs have been successful in indicating the temperature of living cells, showing the potential for a new application of silver nanocluster as a nanothermometer with adjustable response range, bringing novel insight into the regulatory mechanism of AgNC fluorescence variation.

Keywords: fluorescence, DNA-templated silver nanocluster (AgNC), chameleon, temperature-sensitive

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Publication history
Copyright
Acknowledgements

Publication history

Received: 15 May 2017
Revised: 21 August 2017
Accepted: 22 August 2017
Published: 19 March 2018
Issue date: April 2018

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany 2017

Acknowledgements

Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 21375123) and The Ministry of Science and Technology of China (No. 216YFA0203201).

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