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Nano Research 2017, 10(10): 3333-3345 https://doi.org/10.1007/s12274-017-1546-y
Research Article | Issue | Published: 06 June 2017

Biofunctionalized upconverting CaF2: Yb, Tm nanoparticles for Candida albicans detection and imaging

Show Author's Information Małgorzata Misiak1( ),  Michał Skowicki1,  Tomasz Lipiński2,1,  Agnieszka Kowalczyk1,  Katarzyna Prorok1,  Sebastian Arabasz3,1,  Artur Bednarkiewicz1,4
Wrocław Research Centre EIT+, ul. Stabłowicka 147, 54-066Wrocław Poland
Insitute of Immunology and Experimental TherapyPolish Academy of Sciences, ul. Rudolfa Weigla 12, 53-114WrocławPoland
Centre of Polymer and Carbon MaterialsPolish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819ZabrzePoland
Institute of Low Temperature and Structure Research Polish Academy of Sciences, ul. Okólna 2, 50-422Wrocław Poland
Keywords:
nanomaterials, bioimaging, up-conversion luminescence, fluoride nanoparticles
Cite this article:
Misiak M, Skowicki M, Lipiński T, et al. Biofunctionalized upconverting CaF2: Yb, Tm nanoparticles for Candida albicans detection and imaging. Nano Research, 2017, 10(10): 3333-3345. https://doi.org/10.1007/s12274-017-1546-y
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Abstract Full text Electronic supplementary material About this article

Versatile optimization of the synthesis method and composition of Yb3+ and Tm3+ co-doped CaF2 nanoparticles as well as a novel biofunctionalization method were developed and evaluated. Through multistep synthesis, the luminescence intensity of the Tm3+ activator was enhanced by more than 10-fold compared to standard one-step synthesis. The proposed methods were used to homogenously distribute the doping ions within the nanoparticle's volume and thus reduce luminescence quenching. Optimization of dopant ions concentration led to the selection of the most efficient visible and near-infrared up-converting nanoparticles, which were CaF2 doped with 10% Yb3+ 0.05% Tm3+ and 20% Yb3+ 0.5% Tm3+, respectively. To illustrate the suitability of the synthesized nanoparticles as bio-labels, a dedicated biofunctionalization method was used, and the nanoparticles were applied for labeling and imaging of Candida albicans cells. This method shows great promise because of extremely low background and high specificity because of the presence of the attached molecules.


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Electronic supplementary material
About this article

Biofunctionalized upconverting CaF2: Yb, Tm nanoparticles for Candida albicans detection and imaging

Show Author's information Małgorzata Misiak1( ), Michał Skowicki1, Tomasz Lipiński2,1, Agnieszka Kowalczyk1, Katarzyna Prorok1, Sebastian Arabasz3,1, Artur Bednarkiewicz1,4
Wrocław Research Centre EIT+, ul. Stabłowicka 147, 54-066Wrocław Poland
Insitute of Immunology and Experimental TherapyPolish Academy of Sciences, ul. Rudolfa Weigla 12, 53-114WrocławPoland
Centre of Polymer and Carbon MaterialsPolish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819ZabrzePoland
Institute of Low Temperature and Structure Research Polish Academy of Sciences, ul. Okólna 2, 50-422Wrocław Poland

Abstract

Versatile optimization of the synthesis method and composition of Yb3+ and Tm3+ co-doped CaF2 nanoparticles as well as a novel biofunctionalization method were developed and evaluated. Through multistep synthesis, the luminescence intensity of the Tm3+ activator was enhanced by more than 10-fold compared to standard one-step synthesis. The proposed methods were used to homogenously distribute the doping ions within the nanoparticle's volume and thus reduce luminescence quenching. Optimization of dopant ions concentration led to the selection of the most efficient visible and near-infrared up-converting nanoparticles, which were CaF2 doped with 10% Yb3+ 0.05% Tm3+ and 20% Yb3+ 0.5% Tm3+, respectively. To illustrate the suitability of the synthesized nanoparticles as bio-labels, a dedicated biofunctionalization method was used, and the nanoparticles were applied for labeling and imaging of Candida albicans cells. This method shows great promise because of extremely low background and high specificity because of the presence of the attached molecules.

Keywords: nanomaterials, bioimaging, up-conversion luminescence, fluoride nanoparticles

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Publication history
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Acknowledgements

Publication history

Received: 04 January 2017
Revised: 31 January 2017
Accepted: 20 February 2017
Published: 06 June 2017
Issue date: October 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2017

Acknowledgements

Acknowledgements

The research was financed by the Polish National Science Centre grant funded according to the decision no. DEC-2013/11/N/ST5/02716. T. L. acknowledges Polish National Science Centre "OPUS 5" (No. 2013/09/B/NZ6/03526).

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