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Nano Research 2017, 10(8): 2833-2844 https://doi.org/10.1007/s12274-017-1490-x
Research Article | Issue | Published: 03 April 2017

Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods

Show Author's Information Yanni Chen1,2,  Liqiang Liu1,2,  Liguang Xu1,2,  Shanshan Song1,2,  Hua Kuang1,2( ),  Gang Cui1,2,  Chuanlai Xu1,2
State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi214122China
School of Food Science and TechnologyJiangnan UniversityWuxi214122China
Keywords:
monoclonal antibody, sulfonamides, gold immunochromato-graphic sensor, honey samples
Cite this article:
Chen Y, Liu L, Xu L, et al. Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods. Nano Research, 2017, 10(8): 2833-2844. https://doi.org/10.1007/s12274-017-1490-x
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Abstract Full text About this article

A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides. Three haptens (hapten 1 with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain) were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 μg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection (vLOD) for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 μg/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.


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About this article

Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods

Show Author's information Yanni Chen1,2, Liqiang Liu1,2, Liguang Xu1,2, Shanshan Song1,2, Hua Kuang1,2( ), Gang Cui1,2, Chuanlai Xu1,2
State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi214122China
School of Food Science and TechnologyJiangnan UniversityWuxi214122China

Abstract

A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides. Three haptens (hapten 1 with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain) were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 μg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection (vLOD) for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 μg/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.

Keywords: monoclonal antibody, sulfonamides, gold immunochromato-graphic sensor, honey samples

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Publication history
Copyright
Acknowledgements

Publication history

Received: 19 December 2016
Revised: 18 January 2017
Accepted: 19 January 2017
Published: 03 April 2017
Issue date: August 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2017

Acknowledgements

Acknowledgements

This work is financially supported by the Key Programs from MOST (Nos. 2016YFD0401101 and 2016YFF0202300), the National Natural Science Foundation of China (Nos. 21631005, 21522102 and 21503095), and grants from Natural Science Foundation of Jiangsu Province, MOF and MOE (Nos. BE2016307, BK20150138, CMB21S1614, CLE02N1515 and JUSRP51715A).

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