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Nano Research 2017, 10(4): 1345-1355 https://doi.org/10.1007/s12274-017-1446-1
Research Article | Issue | Published: 21 February 2017

Near-infrared (NIR) controlled reversible cell adhesion on a responsive nano-biointerface

Show Author's Information Haijun Cui1,2,  Pengchao Zhang2,3,  Wenshuo Wang1,2,  Guannan Li2,3,  Yuwei Hao2,3,  Luying Wang2,3,  Shutao Wang1,2( )
CAS Key Laboratory of Bio-inspired Materials and Interfacial ScienceCAS Center for Excellence in NanoscienceTechnical Institute of Physics and ChemistryChinese Academy of Sciences (CAS)Beijing100190China
University of Chinese Academy of Sciences (UCAS)Beijing100049China
Beijing National Laboratory for Molecular Sciences (BNLMS)Institute of ChemistryChinese Academy of SciencesBeijing100190China
Keywords:
nanostructures, cell adhesion, photothermal effect, bio-interface, hydrophobic interaction
Cite this article:
Cui H, Zhang P, Wang W, et al. Near-infrared (NIR) controlled reversible cell adhesion on a responsive nano-biointerface. Nano Research, 2017, 10(4): 1345-1355. https://doi.org/10.1007/s12274-017-1446-1
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Abstract Full text Electronic supplementary material About this article

Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared (NIR)- responsive surface for controlling cell adhesion was designed by grafting a thermal responsive polymer (poly(N-isopropylacrylamide), PNIPAM) onto silicon nanowires (SiNWs) instead of the traditional photosensitive moieties. NIR induced the photothermal effect of the SiNWs, and the local heat induced thermodynamic phase transformation of PNIPAM. With the application of NIR radiation, the surface turned to a hydrophobic state, and restored to the hydrophilic state when NIR was switched off, leading to reversible cell adhesion and release. The switchable wettability of the surface and cell adhesion/release occurred efficiently even after 20 cycles. Proteins were anchored on the surface via hydrophobic interactions using NIR; further connection of a cell-capture agent helped in achieving specific cell capture. This dynamic control of cell adhesion via NIR may provide new clues for designing functional nano-biointerfaces.


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Electronic supplementary material
About this article

Near-infrared (NIR) controlled reversible cell adhesion on a responsive nano-biointerface

Show Author's information Haijun Cui1,2, Pengchao Zhang2,3, Wenshuo Wang1,2, Guannan Li2,3, Yuwei Hao2,3, Luying Wang2,3, Shutao Wang1,2( )
CAS Key Laboratory of Bio-inspired Materials and Interfacial ScienceCAS Center for Excellence in NanoscienceTechnical Institute of Physics and ChemistryChinese Academy of Sciences (CAS)Beijing100190China
University of Chinese Academy of Sciences (UCAS)Beijing100049China
Beijing National Laboratory for Molecular Sciences (BNLMS)Institute of ChemistryChinese Academy of SciencesBeijing100190China

Abstract

Light-activated dynamic variations have promoted the development of smart interfaces, especially nano-biointerfaces. In this article, the near-infrared (NIR)- responsive surface for controlling cell adhesion was designed by grafting a thermal responsive polymer (poly(N-isopropylacrylamide), PNIPAM) onto silicon nanowires (SiNWs) instead of the traditional photosensitive moieties. NIR induced the photothermal effect of the SiNWs, and the local heat induced thermodynamic phase transformation of PNIPAM. With the application of NIR radiation, the surface turned to a hydrophobic state, and restored to the hydrophilic state when NIR was switched off, leading to reversible cell adhesion and release. The switchable wettability of the surface and cell adhesion/release occurred efficiently even after 20 cycles. Proteins were anchored on the surface via hydrophobic interactions using NIR; further connection of a cell-capture agent helped in achieving specific cell capture. This dynamic control of cell adhesion via NIR may provide new clues for designing functional nano-biointerfaces.

Keywords: nanostructures, cell adhesion, photothermal effect, bio-interface, hydrophobic interaction

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Publication history
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Acknowledgements

Publication history

Received: 14 October 2016
Revised: 27 December 2016
Accepted: 30 December 2016
Published: 21 February 2017
Issue date: April 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2017

Acknowledgements

Acknowledgements

This research is supported by the National Basic Research Program of China (No. 2012CB933800), National Natural Science Foundation of China (Nos. 21425314, 21501184, 21434009, 21421061 and 21504098), the Key Research Program of the Chinese Academy of Sciences (No. KJZD-EW-M01), the National High-tech R & D Program of China (863 Program) (No. 2013AA032203), MOST (No. 2013YQ190467), the Top-Notch Young Talents Program of China, and Beijing Municipal Science & Technology Commission (No. Z161100000116037).

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