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Nano Research 2017, 10(2): 520-533 https://doi.org/10.1007/s12274-016-1310-8
Research Article | Issue | Published: 13 December 2016

Catheters coated with Zn-doped CuO nanoparticles delay the onset of catheter-associated urinary tract infections

Show Author's Information Yakov Shalom1,  Ilana Perelshtein2,  Nina Perkas2,  Aharon Gedanken2( ),  Ehud Banin1( )
The Mina and Everard Goodman Faculty of Life Sciences and Institute for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel
The Department of Chemistry and Institute for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel
Keywords:
metal oxide, nanoparticle coating, antibiofilm, urinary tract infection, catheter
Cite this article:
Shalom Y, Perelshtein I, Perkas N, et al. Catheters coated with Zn-doped CuO nanoparticles delay the onset of catheter-associated urinary tract infections. Nano Research, 2017, 10(2): 520-533. https://doi.org/10.1007/s12274-016-1310-8
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Abstract Full text Electronic supplementary material About this article

Catheter-associated urinary tract infections (CAUTIs) are among the most common bacterial infections associated with medical devices. In the current study, the synthesis, coating, antibiofilm properties, and biocompatibility of urinary catheters coated with Zn-doped CuO (Zn0.12Cu0.88O) nanoparticles (NPs) were examined. The doped NPs were synthesized and subsequently deposited on the catheter by the sonochemical method. The coated catheters displayed high antibiofilm activity and promising biocompatibility, as indicated by low in vitro cytotoxicity, negligible associated cytokine secretion, and absence of detectable irritation. The biocompatibility and ability of the Zn-doped CuO coating to inhibit biofilm formation were also evaluated in vivo using a rabbit model. Rabbits catheterized with uncoated catheters scored positive for CAUTI by day 4 of the experiment. In contrast, rabbits catheterized with Zn-doped CuO-coated catheters did not exhibit CAUTI until day 7 or remained completely uninfected for the whole duration of the 7-day experiment. Furthermore, the in vivo biocompatibility assays and examinations supported the biosafety of Zn-doped CuO-coated catheters. Taken together, these data highlight the potential of Zn-doped CuO nanocomposite as effective antibiofilm compound.


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Electronic supplementary material
About this article

Catheters coated with Zn-doped CuO nanoparticles delay the onset of catheter-associated urinary tract infections

Show Author's information Yakov Shalom1, Ilana Perelshtein2, Nina Perkas2, Aharon Gedanken2( ), Ehud Banin1( )
The Mina and Everard Goodman Faculty of Life Sciences and Institute for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel
The Department of Chemistry and Institute for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel

Abstract

Catheter-associated urinary tract infections (CAUTIs) are among the most common bacterial infections associated with medical devices. In the current study, the synthesis, coating, antibiofilm properties, and biocompatibility of urinary catheters coated with Zn-doped CuO (Zn0.12Cu0.88O) nanoparticles (NPs) were examined. The doped NPs were synthesized and subsequently deposited on the catheter by the sonochemical method. The coated catheters displayed high antibiofilm activity and promising biocompatibility, as indicated by low in vitro cytotoxicity, negligible associated cytokine secretion, and absence of detectable irritation. The biocompatibility and ability of the Zn-doped CuO coating to inhibit biofilm formation were also evaluated in vivo using a rabbit model. Rabbits catheterized with uncoated catheters scored positive for CAUTI by day 4 of the experiment. In contrast, rabbits catheterized with Zn-doped CuO-coated catheters did not exhibit CAUTI until day 7 or remained completely uninfected for the whole duration of the 7-day experiment. Furthermore, the in vivo biocompatibility assays and examinations supported the biosafety of Zn-doped CuO-coated catheters. Taken together, these data highlight the potential of Zn-doped CuO nanocomposite as effective antibiofilm compound.

Keywords: metal oxide, nanoparticle coating, antibiofilm, urinary tract infection, catheter

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Publication history
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Acknowledgements

Publication history

Received: 27 July 2016
Revised: 26 September 2016
Accepted: 04 October 2016
Published: 13 December 2016
Issue date: February 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Acknowledgements

Acknowledgements

This project was partially funded by a Kamin research grant from the Israeli Ministry of Economy to Ehud Banin. We also thank the Dyna and Fala Weinstock Foundation and the Douer Family Foundation for their support. We are grateful to Dr. Gal Yerushalmi and Yana Belnik for fruitful discussions and assistance in the preparation of the manuscript. We thank Yossi Lavie from Harlan for technical assistance and Dr. Abraham Nyska for histopathological analysis. We thank Katya Gotlib for technical assistance in the HRSEM.

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