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Nano Research 2017, 10(1): 108-120 https://doi.org/10.1007/s12274-016-1270-z
Research Article | Issue | Published: 26 October 2016

Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody

Show Author's Information Juan Peng1,2,  Liqiang Liu1,2,  Liguang Xu1,2,  Shanshan Song1,2,  Hua Kuang1,2( ),  Gang Cui1,2,  Chuanlai Xu1,2
State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,214122,China;
School of Food Science and Technology,Jiangnan University,Wuxi,214122,China;
Keywords:
gold nanoparticle, monoclonal antibody, (fluoro)quinolones, paper sensor
Cite this article:
Peng J, Liu L, Xu L, et al. Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody. Nano Research, 2017, 10(1): 108-120. https://doi.org/10.1007/s12274-016-1270-z
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Abstract Full text About this article

For rapid and simultaneous detection of (fluoro)quinolones, a broadly specific monoclonal antibody (mAb) that recognizes 32 (fluoro)quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles (AuNPs) was then assembled with goat anti-mouse antibody and antigen (sarfloxacin coupled to ovalbumin), used to form the C line and T line, respectively. This antigen competes with the (fluoro)quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 (fluoro)quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1-10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of (fluoro)quinolones in milk.


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About this article

Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody

Show Author's information Juan Peng1,2, Liqiang Liu1,2, Liguang Xu1,2, Shanshan Song1,2, Hua Kuang1,2( ), Gang Cui1,2, Chuanlai Xu1,2
State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,214122,China;
School of Food Science and Technology,Jiangnan University,Wuxi,214122,China;

Abstract

For rapid and simultaneous detection of (fluoro)quinolones, a broadly specific monoclonal antibody (mAb) that recognizes 32 (fluoro)quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles (AuNPs) was then assembled with goat anti-mouse antibody and antigen (sarfloxacin coupled to ovalbumin), used to form the C line and T line, respectively. This antigen competes with the (fluoro)quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 (fluoro)quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1-10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of (fluoro)quinolones in milk.

Keywords: gold nanoparticle, monoclonal antibody, (fluoro)quinolones, paper sensor

References(41)

1

Ceyssens, P.-J.; Van Bambeke, F.; Mattheus, W.; Bertrand, S.; Fux, F.; Van Bossuyt, E.; Damée, S.; Nyssen, H.-J.; De Craeye, S.; Verhaegen, J. et al. Molecular analysis of rising fluoroquinolone resistance in Belgian non-invasive streptococcus pneumoniae isolates (1995-2014). PLoS One 2016, 11, e0154816.
DOI Google Scholar
2

Suryoprabowo, S.; Liu, L. Q.; Peng, J.; Kuang, H.; Xu, C. L. Development of a broad specific monoclonal antibody for fluoroquinolone analysis. Food Anal. Meth. 2014, 7, 2163-2168.
DOI Google Scholar
3

Takahashi, H.; Hayakawa, I.; Akimoto, T. The history of the development and changes of quinolone antibacterial agents. Yakushigaku Zasshi 2003, 38, 161-179.
Google Scholar
4

Zhu, Y.; Li, L.; Wang, Z. H.; Chen, Y. Q.; Zhao, Z. M.; Zhu, L.; Wu, X. P.; Wan, Y. P.; He, F. Y.; Shen, J. Z. Development of an immunochromatography strip for the rapid detection of 12 fluoroquinolones in chicken muscle and liver. J. Agric. Food Chem. 2008, 56, 5469-5474.
DOI Google Scholar
5

Rubinstein, E. History of quinolones and their side effects. Chemotherapy 2001, 47, 3-8.
DOI Google Scholar
6

Wu, X.-L.; Xiang, L.; Yan, Q.-Y.; Jiang, Y.-N.; Li, Y.-W.; Huang, X.-P.; Li, H.; Cai, Q.-Y.; Mo, C.-H. Distribution and risk assessment of quinolone antibiotics in the soils from organic vegetable farms of a subtropical city, Southern China. Sci. Total Environ. 2014, 487, 399-406.
DOI Google Scholar
7

Rutgersson, C.; Fick, J.; Marathe, N.; Kristiansson, E.; Janzon, A.; Angelin, M.; Johansson, A.; Shouche, Y.; Flach, C.-F.; Larsson, D. G. J. Fluoroquinolones and qnr genes in sediment, water, soil, and human fecal flora in an environment polluted by manufacturing discharges. Environ. Sci. Technol. 2014, 48, 7825-7832.
DOI Google Scholar
8

Wang, H. X.; Wang, B.; Zhao, Q.; Zhao, Y. P.; Fu, C. W.; Feng, X.; Wang, N.; Su, M. F.; Tang, C. X.; Jiang, F. et al. Antibiotic body burden of Chinese school children: A multisite biomonitoring-based study. Environ. Sci. Technol. 2015, 49, 5070-5079.
DOI Google Scholar
9

Wang, H. X.; Wang, N.; Wang, B.; Fang, H.; Fu, C. W.; Tang, C. X.; Jiang, F.; Zhou, Y.; He, G. S.; Zhao, Q. et al. Antibiotics detected in urines and adipogenesis in school children. Environ. Int. 2016, 89-90, 204-211.
DOI Google Scholar
10
Codex Alimentarius Commission. Joint FAO/WHO Food Standards Programme; Rome, Italy, 2012.
11
The Ministry of Agriculture. Gazette of the Ministry of Agriculture of The People's Republic of China, No. 2292; Beijing, China, 2015.
12

Hermo, M. P.; Nemutlu, E.; Kir, S.; Barrón, D.; Barbosa, J. Improved determination of quinolones in milk at their MRL levels using LC-UV, LC-FD, LC-MS and LC-MS/MS and validation in line with regulation 2002/657/EC. Anal. Chim. Acta 2008, 613, 98-107.
DOI Google Scholar
13

Hermo, M. P.; Barrón, D.; Barbosa, J. Development of analytical methods for multiresidue determination of quinolones in pig muscle samples by liquid chromatography with ultraviolet detection, liquid chromatography-mass spectrometry and liquid chromagraphy-tandem mass spectrometry. J. Chromatogr. A 2006, 1104, 132-139.
DOI Google Scholar
14

Mirzajani, R.; Kardani, F. Fabrication of ciprofloxacin molecular imprinted polymer coating on a stainless steel wire as a selective solid-phase microextraction fiber for sensitive determination of fluoroquinolones in biological fluids and tablet formulation using HPLC-UV detection. J. Pharm. Biomed. Anal. 2016, 122, 98-109.
DOI Google Scholar
15

Piatkowska, M.; Jedziniak, P.; Zmudzki, J. Multiresidue method for the simultaneous determination of veterinary medicinal products, feed additives and illegal dyes in eggs using liquid chromatography-tandem mass spectrometry. Food Chem. 2016, 197, 571-580.
DOI Google Scholar
16

Wang, Z. H.; Zhu, Y.; Ding, S. Y.; He, F. Y.; Beier, R. C.; Li, J. C.; Jiang, H. Y.; Feng, C. W.; Wan, Y. P.; Zhang, S. X. et al. Development of a monoclonal antibody-based broad-specificity elisa for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds. Anal. Chem. 2007, 79, 4471-4483.
DOI Google Scholar
17

Peng, J.; Kong, D. Z.; Liu, L. Q.; Song, S. S.; Kuang, H.; Xu, C. L. Determination of quinoxaline antibiotics in fish feed by enzyme-linked immunosorbent assay using a monoclonal antibody. Anal. Methods 2015, 7, 5204-5209.
DOI Google Scholar
18

Liu, B.-H.; Chu, K.-C.; Yu, F.-Y. Novel monoclonal antibody-based sensitive enzyme-linked immunosorbent assay and rapid immunochromatographic strip for detecting aflatoxin M1 in milk. Food Control 2016, 66, 1-7.
DOI Google Scholar
19

Cai, M. A.; Li, F.; Zhang, Y.; Wang, Q. B. One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection. Nano Res. 2010, 3, 557-563.
DOI Google Scholar
20

Curnis, F.; Fiocchi, M.; Sacchi, A.; Gori, A.; Gasparri, A.; Corti, A. NGR-tagged nano-gold: A new CD13-selective carrier for cytokine delivery to tumors. Nano Res. 2016, 9, 1393-1408.
DOI Google Scholar
21

Panfilova, E.; Shirokov, A.; Khlebtsov, B.; Matora, L.; Khlebtsov, N. Multiplexed dot immunoassay using Ag nanocubes, Au/Ag alloy nanoparticles, and Au/Ag nanocages. Nano Res. 2012, 5, 124-134.
DOI Google Scholar
22

Huang, P.; Tu, D. T.; Zheng, W.; Zhou, S. Y.; Chen, Z.; Chen, X. Y. Inorganic lanthanide nanoprobes for backgroundfree luminescent bioassays. Sci. China Mater. 2015, 58, 156-177.
DOI Google Scholar
23

Gu, Y.; Meng, G. W.; Wang, M. L.; Huang, Q.; Zhu, C. H.; Huang, Z. L. R6G/8-AQ co-functionalized Fe3O4@SiO2 nanoparticles for fluorescence detection of trace Hg2+ and Zn2+ in aqueous solution. Sci. China Mater. 2015, 58, 550-558.
DOI Google Scholar
24

Li, X.; Song, J.; Chen, B. L.; Wang, B.; Li, R.; Jiang, H. M.; Liu, J. F.; Li, C. Z. A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles. Sci. Bull. 2016, 61, 276-281.
DOI Google Scholar
25

Ma, M.; Chen, H. R.; Shi, J. L. Construction of smart inorganic nanoparticle-based ultrasound contrast agents and their biomedical applications. Sci. Bull. 2015, 60, 1170-1183.
DOI Google Scholar
26

Lin, B. B.; Su, H. Y.; Jin, R. R.; Li, D. Y.; Wu, C. Q.; Jiang, X.; Xia, C. C.; Gong, Q. Y.; Song, B.; Ai, H. Multifunctional dextran micelles as drug delivery carriers and magnetic resonance imaging probes. Sci. Bull. 2015, 60, 1272-1280.
DOI Google Scholar
27

Li, J. X.; Zhao, Y. L. Nanotechnology in the programmed cell therapy: Nowhere to escape of cancer. Sci. Bull. 2016, 61, 45-47.
DOI Google Scholar
28

Li, F. H.; Bao, Y.; Wang, D. D.; Wang, W.; Niu, L. Smartphones for sensing. Sci. Bull. 2016, 61, 190-201.
DOI Google Scholar
29

Fu, C. H.; He, C. F.; Tan, L. F.; Wang, S. H.; Shang, L.; Li, L. L.; Meng, X. W.; Liu, H. Y. High-yield preparation of robust gold nanoshells on silica nanorattles with good biocompatiblity. Sci. Bull. 2016, 61, 282-291.
DOI Google Scholar
30

Xing, C. R.; Liu, L. Q.; Song, S. S.; Feng, M.; Kuang, H.; Xu, C. L. Ultrasensitive immunochromatographic assay for the simultaneous detection of five chemicals in drinking water. Biosens. Bioelectron. 2015, 66, 445-453.
DOI Google Scholar
31

Rivas, L.; de la Escosura-Muñiz, A.; Serrano, L.; Altet, L.; Francino, O.; Sánchez, A.; Merkoçi, A. Triple lines gold nanoparticle-based lateral flow assay for enhanced and simultaneous detection of Leishmania DNA and endogenous control. Nano Res. 2015, 8, 3704-3714.
DOI Google Scholar
32
Wang, W. B.; Liu, L. Q.; Song, S. S.; Xu, L. G.; Kuang, H.; Zhu, J. P.; Xu, C. L. Gold nanoparticle-based strip sensor for multiple detection of twelve salmonella strains with a genus-specific lipopolysaccharide antibody. Sci. China Mater., in press, DOI: 10.1007/s40843-016-5077-0.https://doi.org/10.1007/s40843-016-5077-0
DOI
33

Lee, H.-J.; Ryu, Y.-J.; Tutkun, L.; Park, E.-K. Determination of oxolinic acid residues in the muscle tissue of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay. Food Agric. Immunol. 2016, 27, 367-376.
DOI Google Scholar
34

Zhi, A.-M.; Li, B.-B.; Liu, Q.-T.; Hu, X.-F.; Zhao, D.; Hou, Y.-Z.; Deng, R.-G.; Chai, S.-J.; Zhang, G.-P. Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Food Agric. Immunol. 2010, 21, 335-345.
DOI Google Scholar
35

Zhao, Y. L.; Zhang, G. P.; Liu, Q. T.; Teng, M.; Yang, J. F.; Wang, J. H. Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of enrofloxacin residues. J. Agric. Food Chem. 2008, 56, 12138-12142.
DOI Google Scholar
36

Tochi, B. N.; Peng, J.; Song, S. S.; Liu, L. Q.; Kuang, H.; Xu, C. L. Determination of sarafloxacin and its analogues in milk using an enzyme-linked immunosorbent assay based on a monoclonal antibody. Anal. Methods 2016, 8, 1626-1636.
DOI Google Scholar
37

Li, S.; Xu, L. G.; Ma, W.; Kuang, H.; Wang, L. B.; Xu, C. L. Triple raman label-encoded gold nanoparticle trimers for simultaneous heavy metal ion detection. Small 2015, 11, 3435-3439.
DOI Google Scholar
38

Liu, L. Q.; Luo, L. J.; Suryoprabowo, S.; Peng, J.; Kuang, H.; Xu, C. L. Development of an immunochromatographic strip test for rapid detection of ciprofloxacin in milk samples. Sensors 2014, 14, 16785-16798.
DOI Google Scholar
39

Kong, D. Z.; Liu, L. Q.; Song, S. S.; Suryoprabowo, S.; Li, A. K.; Kuang, H.; Wang, L. B.; Xu, C. L. A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins. Nanoscale 2016, 8, 5245-5253.
DOI Google Scholar
40

Wang, Z. H.; Zhang, H. Y.; Ni, H. J.; Zhang, S. X.; Shen, J. Z. Development of a highly sensitive and specific immunoassay for enrofloxacin based on heterologous coating haptens. Anal. Chim. Acta 2014, 820, 152-158.
DOI Google Scholar
41

Liu, N.; Zhao, Z. Y.; Tan, Y. L.; Lu, L.; Wang, L.; Liao, Y. C.; Beloglazova, N.; De Saeger, S.; Zheng, X. D.; Wu, A. B. Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. Anal. Chem. 2016, 88, 1246-1252.
DOI Google Scholar
Publication history
Copyright
Acknowledgements

Publication history

Received: 02 August 2016
Revised: 25 August 2016
Accepted: 29 August 2016
Published: 26 October 2016
Issue date: January 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Acknowledgements

Acknowledgements

This work is financially supported by the National Natural Science Foundation of China (Nos. 21522102, 21503095, 21471068, 21371081, and 21301073), the Key Programs from MOST (Nos. 2016YFD0401101 and 2012YQ09019410), and grants from Natural Science Foundation of Jiangsu Province, MOF and MOE (Nos. BK20150145, BX20151038, BK20140003, BE2014672, BE2013613, BE2013611).

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