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Nano Research 2015, 8(11): 3512-3523 https://doi.org/10.1007/s12274-015-0851-6
Research Article | Issue | Published: 03 September 2015

Self-assemblies of plasmonic gold/layered double hydroxides with highly efficient antiviral effect against the hepatitis B virus

Show Author's Information Gabriela Carja1( ),  Elena Florentina Grosu1,  Catalina Petrarean2,  Norica Nichita2
Department of Chemical EngineeringFaculty of Chemical Engineering and Environmental ProtectionTechnical University "Gh. Asachi" of IasiBd. Mangeron No. 71Iasi700554Romania
Department of Viral GlycoproteinsInstitute of Biochemistry of the Romanian AcademySplaiul Independentei 296Bucharest060031Romania
Keywords:
layered double hydroxides, layered double hydroxide (LDH), cytotoxicity, plasmonic gold, antiviral effect, HBV, hepatitis
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Carja G, Grosu EF, Petrarean C, et al. Self-assemblies of plasmonic gold/layered double hydroxides with highly efficient antiviral effect against the hepatitis B virus. Nano Research, 2015, 8(11): 3512-3523. https://doi.org/10.1007/s12274-015-0851-6
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Abstract Full text Electronic supplementary material About this article

Engineering complex nanocomposites that specifically target the hepatitis B virus (HBV) and overcome the limitations of current therapies such as limited efficacy and serious side effects is very challenging. Here, for the first time, the antiviral effect of engineered plasmonic gold and layered double hydroxide self-assemblies (AuNPs/LDHs) is demonstrated, using HBV as a model virus and hepatoma-derived HepG2.2.215 cells for viral replication, assembly, and secretion of infectious virions and subviral particles. AuNPs/LDHs were obtained by a simple, cost-effective procedure in which small AuNPs (~3.5 nm) were directly obtained and organized on the surface of larger LDH nanoparticles (~150 nm) by exploiting the capability of MgLDH, ZnLDH, and MgFeLDH to manifest their "structural memory" in the aqueous solution of Au(O2CCH3)3. The self-assembly approach of AuNPs and LDHs was assessed by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (PXRD), and UV—Vis analysis (UV–Vis). All AuNPs/LDHs tested reduced the amount of viral and subviral particles released from treated cells by up to 80% and exhibited good cytocompatibility. AuNPs/MgFeLDH showed the highest antiviral HBV response with more than 90% inhibition of HBV secretion for the whole concentration range. Preliminary studies on the mechanism of HBV inhibition reveals that in the presence of AuNPs/LDHs, HBV particles are sequestered within the treated cells. The antiviral and low cytotoxic plasmonic properties of these Au/LDH nanocomposites indicate that they hold significant potential to be tailored as novel efficient therapeutics for the treatment of hepatitis B.


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Electronic supplementary material
About this article

Self-assemblies of plasmonic gold/layered double hydroxides with highly efficient antiviral effect against the hepatitis B virus

Show Author's information Gabriela Carja1( ), Elena Florentina Grosu1, Catalina Petrarean2, Norica Nichita2
Department of Chemical EngineeringFaculty of Chemical Engineering and Environmental ProtectionTechnical University "Gh. Asachi" of IasiBd. Mangeron No. 71Iasi700554Romania
Department of Viral GlycoproteinsInstitute of Biochemistry of the Romanian AcademySplaiul Independentei 296Bucharest060031Romania

Abstract

Engineering complex nanocomposites that specifically target the hepatitis B virus (HBV) and overcome the limitations of current therapies such as limited efficacy and serious side effects is very challenging. Here, for the first time, the antiviral effect of engineered plasmonic gold and layered double hydroxide self-assemblies (AuNPs/LDHs) is demonstrated, using HBV as a model virus and hepatoma-derived HepG2.2.215 cells for viral replication, assembly, and secretion of infectious virions and subviral particles. AuNPs/LDHs were obtained by a simple, cost-effective procedure in which small AuNPs (~3.5 nm) were directly obtained and organized on the surface of larger LDH nanoparticles (~150 nm) by exploiting the capability of MgLDH, ZnLDH, and MgFeLDH to manifest their "structural memory" in the aqueous solution of Au(O2CCH3)3. The self-assembly approach of AuNPs and LDHs was assessed by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (PXRD), and UV—Vis analysis (UV–Vis). All AuNPs/LDHs tested reduced the amount of viral and subviral particles released from treated cells by up to 80% and exhibited good cytocompatibility. AuNPs/MgFeLDH showed the highest antiviral HBV response with more than 90% inhibition of HBV secretion for the whole concentration range. Preliminary studies on the mechanism of HBV inhibition reveals that in the presence of AuNPs/LDHs, HBV particles are sequestered within the treated cells. The antiviral and low cytotoxic plasmonic properties of these Au/LDH nanocomposites indicate that they hold significant potential to be tailored as novel efficient therapeutics for the treatment of hepatitis B.

Keywords: layered double hydroxides, layered double hydroxide (LDH), cytotoxicity, plasmonic gold, antiviral effect, HBV, hepatitis

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Publication history
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Acknowledgements

Publication history

Received: 12 March 2015
Revised: 01 July 2015
Accepted: 02 July 2015
Published: 03 September 2015
Issue date: November 2015

Copyright

© Tsinghua University Press and Springer‐Verlag Berlin Heidelberg 2015

Acknowledgements

The authors are grateful for the financial supports from the Romanian National Authority for Scientific Research, CNCS-UEFISCDI (No. PN-II-ID-PCE-75/2013) and the Romanian Academy.

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