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Nano Research 2015, 8(7): 2170-2178 https://doi.org/10.1007/s12274-015-0724-z
Research Article | Issue | Published: 11 June 2015

Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy

Show Author's Information Zaixing Jiang1,2,§,  Shuai Zhang2,§,  Chuanxu Yang2,  Jørgen Kjems2,  Yudong Huang1( ),  Flemming Besenbacher2,  Mingdong Dong2( )
Department of Polymer Science and TechnologySchool of Chemical Engineering and TechnologyHarbin Institute of TechnologyHarbin150001China
Interdisciplinary Nanoscience Center (iNANO)Aarhus University, DK-8000, Aarhus CDenmark

§These authors contributed equally to this study.

Keywords:
stability, kinetics, 3D DNA box origami, high-speed AFM, serum
An erratum to this article is available online at https://doi.org/10.1007/s12274-015-0848-1
Cite this article:
Jiang Z, Zhang S, Yang C, et al. Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy. Nano Research, 2015, 8(7): 2170-2178. https://doi.org/10.1007/s12274-015-0724-z
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Abstract Full text Electronic supplementary material About this article

3D DNA origami holds tremendous potential for the encapsulation and selective release of therapeutic drugs. Observations of the real-time performance of these structures in physiological environments will contribute to the development of future applications. We investigated the degradation kinetics of 3D DNA box origami in serum by using high-speed atomic force microscope optimized for imaging 3D DNA origami in real time. The time resolution allowed to characterize the stages of serum effects on individual 3D DNA boxes origami with nanometer resolution. Our results indicate that the digestion process is a combination of rapid collapse and slow degradation phases. Damage to box origami occurs mainly in the collapse phase. Thus, the structural stability of 3D DNA box origami should be improved, especially in the collapse phase, before these structures are used in clinical applications.


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Electronic supplementary material
About this article

Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy

Show Author's information Zaixing Jiang1,2,§, Shuai Zhang2,§, Chuanxu Yang2, Jørgen Kjems2, Yudong Huang1( ), Flemming Besenbacher2, Mingdong Dong2( )
Department of Polymer Science and TechnologySchool of Chemical Engineering and TechnologyHarbin Institute of TechnologyHarbin150001China
Interdisciplinary Nanoscience Center (iNANO)Aarhus University, DK-8000, Aarhus CDenmark

§These authors contributed equally to this study.

Abstract

3D DNA origami holds tremendous potential for the encapsulation and selective release of therapeutic drugs. Observations of the real-time performance of these structures in physiological environments will contribute to the development of future applications. We investigated the degradation kinetics of 3D DNA box origami in serum by using high-speed atomic force microscope optimized for imaging 3D DNA origami in real time. The time resolution allowed to characterize the stages of serum effects on individual 3D DNA boxes origami with nanometer resolution. Our results indicate that the digestion process is a combination of rapid collapse and slow degradation phases. Damage to box origami occurs mainly in the collapse phase. Thus, the structural stability of 3D DNA box origami should be improved, especially in the collapse phase, before these structures are used in clinical applications.

Keywords: stability, kinetics, 3D DNA box origami, high-speed AFM, serum

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Publication history
Copyright
Acknowledgements

Publication history

Received: 07 December 2014
Revised: 09 January 2015
Accepted: 12 January 2015
Published: 11 June 2015
Issue date: July 2015

Copyright

© Tsinghua University Press and Springer‐Verlag Berlin Heidelberg 2015

Acknowledgements

Acknowledgements

The authors acknowledge financial support from CDNA Centre through the Danish National Research Foundation and the financial support from iNANO Center through the Danish Research Agency, the Carlsberg Foundation, and the Villum Foundation. The authors would like to thank UTC Exploration Project (CASC-HIT12-1C03), Opening Project of Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education (No. 12zxgkI0) and Harbin city science and technology projects (No. 2013DB4BP031).

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