Novel nanocomposites based on a strawberry-like gold- coated magnetite (Fe@Au) for protein separation in multiple myeloma serum samples
),
Javier Fernández-Lodeiro1,2,4(
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A new process to produce magnetite partially coated with strawberry-like gold nanoparticles in aqueous media is reported. The fast response to magnetic fields and optical properties of gold nanoparticle-based colloidal systems are the two main advantages of this new Fe@Au nanomaterial. These advantages allow for the use of this new colloidal nanomaterial for various purposes in proteomics and biomedicine, as proteins can bind to the surface, and the surface can also be functionalized. As proof-of-concept, the new Fe@Au nanoparticles have been assessed in biomarker discovery as a tool for pre-concentration and separation of proteins from complex proteomes. To this end, sera from healthy people were compared with sera from patients diagnosed with multiple myeloma. The application of this new Fe@Au nanomaterial combined with mass spectrometry has allowed for the identification of 53 proteins, and it has also shown that the heat shock protein HSP75 and the plasma protease C1 inhibitor are potential biomarkers for diagnostics and control of multiple myeloma progression.
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Novel nanocomposites based on a strawberry-like gold- coated magnetite (Fe@Au) for protein separation in multiple myeloma serum samples
), Javier Fernández-Lodeiro1,2,4(
)
Abstract
A new process to produce magnetite partially coated with strawberry-like gold nanoparticles in aqueous media is reported. The fast response to magnetic fields and optical properties of gold nanoparticle-based colloidal systems are the two main advantages of this new Fe@Au nanomaterial. These advantages allow for the use of this new colloidal nanomaterial for various purposes in proteomics and biomedicine, as proteins can bind to the surface, and the surface can also be functionalized. As proof-of-concept, the new Fe@Au nanoparticles have been assessed in biomarker discovery as a tool for pre-concentration and separation of proteins from complex proteomes. To this end, sera from healthy people were compared with sera from patients diagnosed with multiple myeloma. The application of this new Fe@Au nanomaterial combined with mass spectrometry has allowed for the identification of 53 proteins, and it has also shown that the heat shock protein HSP75 and the plasma protease C1 inhibitor are potential biomarkers for diagnostics and control of multiple myeloma progression.
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Acknowledgements
All authors thank the Scientific Association PROTEOMASS (Portugal) for financial support. H.M.S. thanks the FCT-MCTES (Portugal) for his postdoctoral grant (reference SRFH/BPD/73997/2010). J.F.L. acknowledges FAPESP grants 2013/11666-0 (FAPESP Brazil), and 150466/2014-5 (CNPq-Brazil) for his postdoctoral Fellowships. C.L. and J.L.C. thank REQUIMTE-FCT PEst-C/EQB/LA0006/2013 for funding. The authors are grateful to the financial and structural support offered by the University of São Paulo (Brazil) through the NAP-CatSinQ (Research Core in Catalysis and Chemical Synthesis), FAPESP (2008/55401-1 and 2011/11613-8) and CNPq (401797/2013-9, 150466/ 2014-5, 311152/2013-9) grants. C.L. and A.A.S. acknowledge the Science Without Borders Grant (CNPq 313746/2013-3).
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