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Nano Research 2014, 7(5): 755-764 https://doi.org/10.1007/s12274-014-0436-9
Research Article | Issue | Published: 24 April 2014

Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles

Show Author's Information Qian Liu1,4,  Jingyi Li1,4,  Hongxue Liu5,  Ibrahim Tora1,  Matthew S. Ide6,  Jiwei Lu5,  Robert J. Davis6,  David L. Green6,  James P. Landers1,2,3,4( )
Department of Chemistry, University of Virginia McCormick Road, P. O. Box 400319, CharlottesvilleVirginia22904 USA
Department of Pathology University of Virginia Health Science CenterCharlottesvilleVirginia 22908 USA
Department of Mechanical Engineering, University of Virginia CharlottesvilleVirginia22904 USA
Center for Microsystems for the Life Sciences, University of Virginia CharlottesvilleVirginia22904 USA
Department of Materials Science & Engineering, University of VirginiaP. O. Box 400745, 395 McCormick RoadCharlottesvilleVirginia22904-4745 USA
Department of Chemical Engineering, University of Virginia123 Engineers' WayCharlottesvilleVirginia22904 USA
Keywords:
core-shell, superparamagnetic, silica/magnetite, DNA quantification, polymerase chain reaction (PCR)
Cite this article:
Liu Q, Li J, Liu H, et al. Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles. Nano Research, 2014, 7(5): 755-764. https://doi.org/10.1007/s12274-014-0436-9
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Abstract Full text Electronic supplementary material About this article

DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO2-coated Fe3O4 (Fe3O4@SiO2) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ~200 nm in diameter with a large surface area (141.89 m2/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe3O4@SiO2 nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.


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Electronic supplementary material
About this article

Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles

Show Author's information Qian Liu1,4, Jingyi Li1,4, Hongxue Liu5, Ibrahim Tora1, Matthew S. Ide6, Jiwei Lu5, Robert J. Davis6, David L. Green6, James P. Landers1,2,3,4( )
Department of Chemistry, University of Virginia McCormick Road, P. O. Box 400319, CharlottesvilleVirginia22904 USA
Department of Pathology University of Virginia Health Science CenterCharlottesvilleVirginia 22908 USA
Department of Mechanical Engineering, University of Virginia CharlottesvilleVirginia22904 USA
Center for Microsystems for the Life Sciences, University of Virginia CharlottesvilleVirginia22904 USA
Department of Materials Science & Engineering, University of VirginiaP. O. Box 400745, 395 McCormick RoadCharlottesvilleVirginia22904-4745 USA
Department of Chemical Engineering, University of Virginia123 Engineers' WayCharlottesvilleVirginia22904 USA

Abstract

DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO2-coated Fe3O4 (Fe3O4@SiO2) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ~200 nm in diameter with a large surface area (141.89 m2/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe3O4@SiO2 nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.

Keywords: core-shell, superparamagnetic, silica/magnetite, DNA quantification, polymerase chain reaction (PCR)

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Publication history
Copyright
Acknowledgements

Publication history

Received: 11 October 2013
Revised: 17 January 2014
Accepted: 27 February 2014
Published: 24 April 2014
Issue date: May 2014

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014

Acknowledgements

Acknowledgements

We would like to acknowledge Dr. Michal Sabat and Richard R. White from University of Virginia Nanoscale Materials Characterization Facility for their help in making this research possible.

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