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Nano Research 2013, 6(2): 113-120 https://doi.org/10.1007/s12274-012-0286-2
Research Article | Issue | Published: 22 December 2012

Multiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence

Show Author's Information Bo Zhang1,  Jordan Price2,  Guosong Hong1,  Scott M. Tabakman1,  Hailiang Wang1,  Justin A. Jarrell2,  Ju Feng1,  Paul J. Utz2,3,  Hongjie Dai1( )
Department of ChemistryStanford UniversityStanford, CaliforniaUSA
Department of MedicineDivision of Immunology and RheumatologyStanford University School of MedicineStanford, CaliforniaUSA
Institute for ImmunityTransplantation and InfectionStanford University School of MedicineStanford, CaliforniaUSA
Keywords:
plasmonic, microarray, cytokine, multiplex, near infrared fluorescence
Cite this article:
Zhang B, Price J, Hong G, et al. Multiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence. Nano Research, 2013, 6(2): 113-120. https://doi.org/10.1007/s12274-012-0286-2
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Abstract Full text Electronic supplementary material About this article

Protein microarrays based on fluorescence detection have been widely utilized for high-throughput functional proteomic analysis. However, a drawback of such assays has been low sensitivity and narrow dynamic range, limiting their capabilities, especially for detecting low abundance biological molecules such as cytokines in human samples. Here, we present fluorescence-enhancing microarrays on plasmonic gold films for multiplexed cytokine detection with up to three orders of magnitude higher sensitivity than on conventional0020nitrocellulose and glass substrates. Cytokine detection on the gold plasmonic substrate is about one to two orders of magnitude more sensitive than enzyme-linked immunosorbent assay (ELISA) and can be multiplexed. A panel of six cytokines (Vascular endothelial growth factor (VEGF), Interleukin 1β (IL-1β), Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interferon γ (IFN-γ), and Tumor necrosis factor (TNF)) were detected in the culture media of cancer cells. This work establishes a new method of high throughput multiplexed cytokine detection with higher sensitivity and dynamic range than ELISA.


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Electronic supplementary material
About this article

Multiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence

Show Author's information Bo Zhang1, Jordan Price2, Guosong Hong1, Scott M. Tabakman1, Hailiang Wang1, Justin A. Jarrell2, Ju Feng1, Paul J. Utz2,3, Hongjie Dai1( )
Department of ChemistryStanford UniversityStanford, CaliforniaUSA
Department of MedicineDivision of Immunology and RheumatologyStanford University School of MedicineStanford, CaliforniaUSA
Institute for ImmunityTransplantation and InfectionStanford University School of MedicineStanford, CaliforniaUSA

Abstract

Protein microarrays based on fluorescence detection have been widely utilized for high-throughput functional proteomic analysis. However, a drawback of such assays has been low sensitivity and narrow dynamic range, limiting their capabilities, especially for detecting low abundance biological molecules such as cytokines in human samples. Here, we present fluorescence-enhancing microarrays on plasmonic gold films for multiplexed cytokine detection with up to three orders of magnitude higher sensitivity than on conventional0020nitrocellulose and glass substrates. Cytokine detection on the gold plasmonic substrate is about one to two orders of magnitude more sensitive than enzyme-linked immunosorbent assay (ELISA) and can be multiplexed. A panel of six cytokines (Vascular endothelial growth factor (VEGF), Interleukin 1β (IL-1β), Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interferon γ (IFN-γ), and Tumor necrosis factor (TNF)) were detected in the culture media of cancer cells. This work establishes a new method of high throughput multiplexed cytokine detection with higher sensitivity and dynamic range than ELISA.

Keywords: plasmonic, microarray, cytokine, multiplex, near infrared fluorescence

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Publication history
Copyright
Acknowledgements

Publication history

Received: 12 November 2012
Revised: 01 December 2012
Accepted: 05 December 2012
Published: 22 December 2012
Issue date: February 2013

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2012

Acknowledgements

Acknowledgements

This work was supported by National Institutes of Health-National Cancer Institute (NIH-NCI) No. 5R01CA135109-02.

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