A Facile Assay for Direct Colorimetric Visualization of Lipopolysaccharides at Low Nanomolar Level
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We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteamine-modified gold nanoparticles (CSH–Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3.3 × 10−10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far.
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A Facile Assay for Direct Colorimetric Visualization of Lipopolysaccharides at Low Nanomolar Level
),
Abstract
We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteamine-modified gold nanoparticles (CSH–Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3.3 × 10−10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 21073213, 60978034 and 20903110), the Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences, and the National High Technology Research and Development Program of China (863 Program) (Grant No. 2009AA03Z318).
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