Journal Home > Volume 1 , Issue 6
Nano Research 2008, 1(6): 490-496 https://doi.org/10.1007/s12274-008-8052-1
Research Article | Open Access | Issue | Published: 01 December 2008

A Nano- and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network

Show Author's Information Juan Yan1,  Mei Hu2,  Di Li1,  Yao He1,2,  Rui Zhao3,  Xingyu Jiang3,  Shiping Song1( ),  Lianhui Wang2( ),  Chunhai Fan1( )
Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800 China
Laboratory of Advanced Materials (LAM) Fudan University, 220 Handan RoadShanghai 200433 China
National Center for NanoScience and Technology, No. 11, Beiyitiao, Zhongguancun Beijing 100080 China
Keywords:
Quantum dots, microfluidics, polydimethylsiloxane (PDMS), carcinoma embryonic antigen (CEA), microarray
Cite this article:
Yan J, Hu M, Li D, et al. A Nano- and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network. Nano Research, 2008, 1(6): 490-496. https://doi.org/10.1007/s12274-008-8052-1
Download citation
EndNote(RIS)
BibTeX

584

Views

28

Downloads

Citations

49

Crossref

N/A

WoS

0

Scopus

0

CSCD

Abstract Full text Electronic supplementary material About this article

A novel nano- and micro-integrated protein chip (NMIPC) that can detect proteins with ultrahigh sensitivity has been fabricated. A microfluidic network (μFN) was used to construct the protein chips, which allowed facile patterning of proteins and subsequent biomolecular recognition. Aqueous phase-synthesized, water-soluble fluorescent CdTe/CdS core-shell quantum dots (aqQDs), having high quantum yield and high photostability, were used as the signaling probe. Importantly, it was found that aqQDs were compatible with microfluidic format assays, which afforded highly sensitive protein chips for cancer biomarker assays.


menu
Abstract
Full text
Outline
Electronic supplementary material
About this article

A Nano- and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network

Show Author's information Juan Yan1, Mei Hu2, Di Li1, Yao He1,2, Rui Zhao3, Xingyu Jiang3, Shiping Song1( ), Lianhui Wang2( ), Chunhai Fan1( )
Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800 China
Laboratory of Advanced Materials (LAM) Fudan University, 220 Handan RoadShanghai 200433 China
National Center for NanoScience and Technology, No. 11, Beiyitiao, Zhongguancun Beijing 100080 China

Abstract

A novel nano- and micro-integrated protein chip (NMIPC) that can detect proteins with ultrahigh sensitivity has been fabricated. A microfluidic network (μFN) was used to construct the protein chips, which allowed facile patterning of proteins and subsequent biomolecular recognition. Aqueous phase-synthesized, water-soluble fluorescent CdTe/CdS core-shell quantum dots (aqQDs), having high quantum yield and high photostability, were used as the signaling probe. Importantly, it was found that aqQDs were compatible with microfluidic format assays, which afforded highly sensitive protein chips for cancer biomarker assays.

Keywords: Quantum dots, microfluidics, polydimethylsiloxane (PDMS), carcinoma embryonic antigen (CEA), microarray

References(35)

1

Ekins, R.; Chu, F. W. Microarrays: Their origins and applications. Trends Biotechnol. 1999, 17, 217–218.
DOI Google Scholar
2

MacBeath, G.; Schreiber, S. L. Printing proteins as microarrays for high-throughput function determination. Science 2000, 289, 1760–1763.
Google Scholar
3

Lueking, A.; Horn, M.; Eickhoff, H.; Bussow, K.; Lehrach, H.; Walter, G. Protein microarrays for gene expression and antibody screening. Anal. Biochem. 1999, 270, 103–111.
DOI Google Scholar
4

Hultschig, C.; Kreutzberger, J.; Seitz, H.; Konthur, Z.; Bussow, K.; Lehrach, H. Recent advances of protein microarrays. Curr. Opin. Chem. Biol. 2006, 10, 4–10.
DOI Google Scholar
5

Sauer, S.; Lange, B. M.; Gobom, J.; Nyarsik, L.; Seitz, H.; Lehrach, H. Miniaturization in functional genomics and proteomics. Nat. Rev. Genet. 2005, 6, 465–476.
DOI Google Scholar
6

Templin, M. F.; Stoll, D.; Schwenk, J. M.; Potz, O.; Kramer, S.; Joos, T. O. Protein microarrays: Promising tools for proteomic research. Proteomics 2003, 3, 2155–2166.
DOI Google Scholar
7

Haab, B. B. Methods and applications of antibody microarrays in cancer research. Proteomics 2003, 3, 2116–2122.
DOI Google Scholar
8

Eisenstein, M. Protein arrays – Growing pains. Nature 2006, 444, 959–962.
DOI Google Scholar
9

Bruchez, M., Jr.; Moronne, M.; Gin, P.; Weiss, S.; Alivisatos, A. P. Semiconductor nanocrystals as fluorescent biological labels. Science 1998, 281, 2013–2016.
DOI Google Scholar
10

Chan, W. C.; Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281, 2016–2018.
DOI Google Scholar
11

Medintz, I. L.; Uyeda, H. T.; Goldman, E. R.; Mattoussi, H. Quantum dot bioconjugates for imaging, labelling and sensing. Nat. Mater. 2005, 4, 435–446.
DOI Google Scholar
12

Michalet, X.; Pinaud, F. F.; Bentolila, L. A.; Tsay, J. M.; Doose, S.; Li, J. J.; Sundaresan, G.; Wu, A. M.; Gambhir, S. S.; Weiss, S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 2005, 307, 538–544.
DOI Google Scholar
13

Peng, X. G.; Manna, L.; Yang, W. D.; Wickham, J.; Scher, E.; Kadavanich, A.; Alivisatos, A. P. Shape control of CdSe nanocrystals. Nature 2000, 404, 59–61.
DOI Google Scholar
15

Alivisatos, A. P. Semiconductor clusters, nanocrystals, and quantum dots. Science 1996, 271, 933–937.
DOI Google Scholar
16

Battaglia, D.; Peng, X. G. Formation of high quality InP and InAs nanocrystals in a noncoordinating solvent. Nano Lett. 2002, 2, 1027–1030.
DOI Google Scholar
17

Tang, Z. Y.; Kotov, N. A.; Giersig, M. Spontaneous organization of single CdTe nanoparticles into luminescent nanowires. Science 2002, 297, 237–240
DOI Google Scholar
18

Qu, L.; Peng, X. G. Control of photoluminescence properties of CdSe nanocrystals in growth. J. Am. Chem. Soc. 2002, 124, 2049–2055.
DOI Google Scholar
19

Bakalova, R.; Zhelev, Z.; Ohba, H.; Baba, Y. Quantum dot-based western blot technology for ultrasensitive detection of tracer proteins. J. Am. Chem. Soc. 2005, 127, 9328–9329.
DOI Google Scholar
20

Wuister, S. F.; Swart, I.; Driel, F. V.; Hickey, S. G.; Donega, C. M. Highly luminescent water-soluble CdTe quantum dots. Nano Lett. 2003, 3, 503–507.
DOI Google Scholar
21

Wang, Y.; Tang, Z.; Correa-Duarte, M. A.; Pastoriza-Santos, I.; Giersig, M.; Kotov, N. A.; Liz-Marzan, L. M. Mechanism of strong luminescence photoactivation of citrate-stabilized water-soluble nanoparticles with CdSe cores. J. Phys. Chem. B 2004, 108, 15461–15469.
DOI Google Scholar
22

Bao, H.; Gong, Y.; Li, Z.; Gao, M. Enhancement effect of illumination on the photoluminescence of water-soluble CdTe nanocrystals: Toward highly fluorescent CdTe/CdS core-shell structure. Chem. Mater. 2004, 16, 3853–3859.
DOI Google Scholar
23

He, Y.; Lu, H.; Sai, L.; Su, Y.; Hu, M.; Fan, C.; Huang, W.; Wang, L. Enhancement effect of illumination on the photoluminescence of water-soluble CdTe nanocrystals: Toward highly fluorescent CdTe/CdS core-shell structure. Adv. Mater. 2008, 20, 3416–3421.
DOI Google Scholar
24

He, Y.; Lu, H.; Sai, L.; Lai, W.; Fan, Q.; Wang, L.; Huang, W. Microwave-assisted growth and characterization of water-dispersed CdTe/CdS core-shell nanocrystals with high photoluminescence. J. Phys. Chem. B 2006, 110, 13370–13374.
DOI Google Scholar
25

Balboni, I.; Chan, S. M.; Kattah, M.; Tenenbaum, J. D.; Butte, A. J.; Utz, P. J. Multiplexed protein array platforms for analysis of autoimmune diseases. Annu. Rev. Immunol. 2006, 24, 391–418.
DOI Google Scholar
26

Chan, S. M.; Ermann, J.; Su, L.; Fathman, C. G.; Utz, P. J. Protein microarrays for multiplex analysis of signal transduction pathways. Nat. Med. 2004, 10, 1390–1396.
DOI Google Scholar
27

Deng, Y.; Zhu, X. Y.; Kienlen, T.; Guo, A. Transport at the air/water interface is the reason for rings in protein microarrays. J. Am. Chem. Soc. 2006, 128, 2768–2769.
DOI Google Scholar
28

Jiang, X. Y.; Xu, Q.; Dertinger, S. K. W.; Stroock, A. D.; Fu, T.; Whitesides, G. M. A general method for patterning gradients of biomolecules on surfaces using microfluidic networks. Anal. Chem. 2005, 77, 2338–2347.
DOI Google Scholar
29

McDonald, J. C.; Duffy, D. C.; Anderson, J. R.; Chiu, D. T.; Wu, H.; Schueller, O. J.; Whitesides, G. M. Fabrication of microfluidic systems in poly(dimethylsiloxane). Electrophoresis 2000, 21, 27–40.
DOI
30

Whitesides, G. M. The origins and the future of microfluidics. Nature 2006, 442, 368–373.
DOI Google Scholar
31

Psaltis, D.; Quake, S. R.; Yang, C. Developing optofluidic technology through the fusion of microfluidics and optics. Nature 2006, 442, 381–386.
DOI Google Scholar
32

Bernard, A.; Michel, B.; Delamarche, E. Micromosaic immunoassays. Anal. Chem. 2001, 73, 8–12.
DOI Google Scholar
33

Wolf, M.; Juncker, D.; Michel, B.; Hunziker, P.; Delamarche, E. Simultaneous detection of C-reactive protein and other cardiac markers in human plasma using micromosaic immunoassays and self-regulating microfluidic networks. Biosens. Bioelectron. 2004, 19, 1193–1202.
DOI Google Scholar
34

Gyorvary, E. S.; O'Riordan, A.; Quinn, A. J.; Redmond, G.; Pum, D.; Sleyt, U. B. Biomimetic nanostructure fabrication: Nonlithographic lateral patterning and self-assembly of functional bacterial S-layers at silicon supports. Nano Lett. 2003, 3, 315–319.
DOI Google Scholar
35

Goldman, E. R.; Balighian, E. D.; Mattoussi, H.; Kuno, M. K.; Mauro, J. M.; Tran, P. T.; Anderson, G. P.; Avidin: A natural bridge for quantum dot-antibody conjugates. J. Am. Chem. Soc. 2002, 124, 6378–6382.
DOI Google Scholar
36

Jaiswal, J. K.; Goldman, E. R.; Simon, S. M. Use of quantum dots for live cell imaging. Nat. Methods 2004, 1, 73–78.
DOI Google Scholar
File
nr-1-6-490_ESM.pdf (350.3 KB)
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 19 August 2008
Revised: 03 November 2008
Accepted: 03 November 2008
Published: 01 December 2008
Issue date: December 2008

Copyright

© Tsinghua Press and Springer-Verlag 2008

Acknowledgements

Acknowledgements

This work was supported by the National Natural Science Foundation (20873175 and 20725516), the Ministry of Science and Technology (2006CB933000, 2007CB936000, and 2007AA06A406), and the Shanghai Municipal Commission for Science and Technology (0852nm00400, 0752nm021).

Rights and permissions

This article is published with open access at Springerlink.com

Return