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Original Article | Open Access

Analysis of the Serum MicroRNA Expression Profiles in Patients With Type 2 Diabetic Nephropathy After Simultaneous Pancreas‐Kidney Transplantation

Luhao Liu1, Chaoqun Yu2, Peng Zhang1, Rongxin Chen1, Yuhe Guo1, Jiali Fang1, Junjie Ma1, Guanghui Li1( ), Zheng Chen1 ( )
Department of Organ Transplantation, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China
Department of Operating Room, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China

Luhao Liu, Chaoqun Yu, and Peng Zhang contributed equally to this study.

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Abstract

Background

Simultaneous pancreas‐kidney (SPK) transplantation has become a standard therapy for end‐stage diabetic nephropathy patients. This study aims to investigate serum microRNA (miRNA) profiles in patients with type 2 diabetic nephropathy after SPK and explored the mechanism of insulin resistance induced by relevant miRNAs.

Methods

Patients with type 2 diabetic nephropathy were recruited at our organ transplantation center from June 2018 to March 2019. Six patients were randomly selected, and miRNA microarray analysis was performed to assess serum miRNA expression levels. Target genes were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was conducted.

Results

The gene chip results revealed significant upregulation of miR‐320d, miR‐4701‐3p, and miR‐940 and significant downregulation of miR‐1229‐5p, miR‐125a‐3p, miR‐3614‐5p, miR‐6090, and miR‐6893‐5p. Target gene prediction and pathway analysis revealed that four miRNAs (miR‐125a‐3p, miR‐320d, miR‐940, and miR‐1229‐5p) form distinct signal regulatory networks.

Conclusion

Specific miRNA expression profiles are present in the serum of patients with diabetic nephropathy after simultaneous pancreas‐kidney transplantation. These specific miRNAs may be involved in the regulatory mechanism of restoring normoglycemia in diabetic nephropathy patients.

Graphical Abstract

In this study, gene chip technology was used to analyze differentially expressed miRNAs in type 2 diabetic nephropathy patients pre‐ and post‐simultaneous pancreas‐kidney transplantation. The results may enhance understanding of type 2 diabetes and insulin resistance mechanisms.

Keywords

diabetic nephropathygene chipmicroRNAsimultaneous pancreas and kidney transplantation

References

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Organ Medicine
Volume 1 Issue 2,
December 2024
Pages 62-69
DOI: 10.1002/orm2.16

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Cite this article:
Liu L, Yu C, Zhang P, et al. Analysis of the Serum MicroRNA Expression Profiles in Patients With Type 2 Diabetic Nephropathy After Simultaneous Pancreas‐Kidney Transplantation. Organ Medicine, 2024, 1(2): 62-69. https://doi.org/10.1002/orm2.16

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Received: 27 August 2024
Revised: 27 November 2024
Accepted: 07 December 2024
Published: 27 December 2024
© 2024 The Author(s).

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.