@article{ZHANG2026, 
author = {Hui-Hong ZHANG and Hao-Yuan XU and Xiu-Xia GUO and Peng CHENG and Hai-Fang WANG and Li-Juan LIU and Chong-Xing ZHANG},
title = {Diversity and composition analysis of cuticle surface and internal bacterial communities of laboratory-reared field F1 Culex pipiens pallens},
year = {2026},
journal = {Journal of Environmental Entomology},
volume = {48},
number = {3},
pages = {864-872},
keywords = {Culex pipiens pallens, 16S rRNA, high-throughput sequencing, diversity, bacterial community},
url = {https://www.sciopen.com/article/10.3969/j.issn.1674-0858.2026.03.21},
doi = {10.3969/j.issn.1674-0858.2026.03.21},
abstract = {AimThis study investigated the diversity and composition of cuticle surface and internal bacterial communities in larvae of a laboratory strain (SS) versus laboratory-reared F1 larvae derived from wild Culex pipiens pallens populations.MethodsThe findings provided insights into how laboratory colonization influence the bacterial communities of wild mosquitoes. Larvae of Cx. pipiens pallens were collected from Tangkou Town (TK) and Weishan Lake (WSH) in Jining City, Shandong Province, in October 2023, and were subsequently reared in the laboratory to produce the F1 progeny. 16S rRNA sequencing was used to analyse the diversity and composition of the cuticle surface and internal bacterial communities in laboratory strain and laboratory-reared field F1 larvae maintained under identical conditions.MethodsThe results showed that the dominant bacterial phyla of the larval cuticle surfaces and internal bacterial communities were Proteobacteria, Actinobacteriota and Firmicutes. Alpha diversity analyses indicated no statistically significant differences among the cuticle surface bacterial communities of the SS, TK and WSH groups, whereas the internal bacterial diversity in the TK group was significantly higher than that in the SS group. Beta diversity analysis showed significant differences in the cuticle surface bacterial community composition between the SS and WSH groups (P=0.024), and significant differences were also observed in the internal bacterial communities between the laboratory strain and the field-derived F1 larvae (TK: P=0.012, WSH: P=0.022). Linear discriminant analysis Effect Size (LEfSe) demonstrated that the significantly different bacterial taxa on the larval cuticle surfaces among the three groups belonged to 1 phylum, 3 classes, 4 orders, 5 families, and 6 genera. In contrast, the significantly different bacterial taxa in the internal bacterial communities of larvae belonged to 1 phylum, 1 class, 4 orders, 5 families, and 7 genera.ConclusionIn summary, under standard laboratory rearing conditions, the native bacterial communities from field populations of Cx. pipiens pallens were partially transmitted to their laboratory-reared F1 offspring larvae.}
}