@article{Zhang2024, 
author = {Xinyue Zhang and Jiahua Huang and Jing Zhao and Lisha Li and Fengze Miao and Tingrui Zhang and Zhongjian Chen and Xing Zhou and Zongguang Tai and Quangang Zhu},
title = {Exosome-mimetic vesicles derived from fibroblasts carrying matrine for wound healing},
year = {2024},
journal = {Burns & Trauma},
volume = {12},
pages = {tkae015},
keywords = {Wound healing, Exosome-mimetic vesicles, Matrine, Anti-inflammatory effect, Angiogenesis},
url = {https://www.sciopen.com/article/10.1093/burnst/tkae015},
doi = {10.1093/burnst/tkae015},
abstract = {BackgroundChronic skin wounds are a leading cause of hospital admissions and reduced life expectancy among older people and individuals with diabetes. Delayed wound healing is often attributed to a series of cellular abnormalities. Matrine, a well-studied component found in Sophora flavescens, is recognized for its anti-inflammatory effects. However, its impact on wound healing still remains uncertain. This study aims to explore the potential of matrine in promoting wound healing.MethodsIn this study, we utilized gradient extrusion to produce fibroblast-derived exosome-mimetic vesicles as carriers for matrine (MHEM). MHEM were characterized using transmission electron microscopy and dynamic light scattering analysis. The therapeutic effect of MHEM in wound healing was explored in vitro and in vivo.ResultsBoth matrine and MHEM enhanced the cellular activity as well as the migration of fibroblasts and keratinocytes. The potent anti-inflammatory effect of matrine diluted the inflammatory response in the vicinity of wounds. Furthermore, MHEM worked together to promote angiogenesis and the expression of transforming growth factor β and collagen I. MHEM contained growth factors of fibroblasts that regulated the functions of fibroblasts, keratinocytes and monocytes, which synergistically promoted wound healing with the anti-inflammatory effect of matrine.ConclusionsMHEM showed enhanced therapeutic efficacy in the inflammatory microenvironment, for new tissue formation and angiogenesis of wound healing.}
}