TY  - JOUR
AU  - Ding, Xiaotong
AU  - Yang, Chenxi
AU  - Li, Yue
AU  - He, Tangtang
AU  - Xu, Yan
AU  - Cheng, Xuxi
AU  - Song, Jinyun
AU  - Xue, Nannan
AU  - Min, Wen
AU  - Feng, Weimeng
AU  - Zhao, Hongyu
AU  - Dong, Jie
AU  - Liu, Pei
AU  - Wang, Yiwei
AU  - Chen, Jun
PY  - 2024
TI  - Reshaped commensal wound microbiome via topical application of Calvatia gigantea extract contributes to faster diabetic wound healing
JO  - Burns & Trauma
SN  - 2321-3868
SP  - tkae037
VL  - 12
AB  - BackgroundCalvatia gigantea (CG) is widely used as a traditional Chinese medicine for wound treatment. In this study, we aimed to determine the effects of CG extract (CGE) on diabetic wound healing and the commensal wound microbiome.MethodA wound model was established using leptin receptor-deficient db/db mice, with untreated mice as the control group and CGE-treated mice as the treatment group. The wound healing rate, inflammation and histology were analyzed. Additionally, wound microbiome was evaluated via 16S ribosomal RNA (rRNA) gene sequencing.ResultsCGE significantly accelerated the healing of diabetic ulcer wounds, facilitated re-epithelialization, and downregulated the transcription levels of the inflammatory cytokines, interleukin-1β and tumor necrosis factor-α. Furthermore, CGE treatment positively affected the wound microbiome, promoting diversity of the microbial community and enrichment of Escherichia–Shigella bacteria in the CGE-treated group.ConclusionsOverall, CGE enhanced diabetic wound healing by modulating the wound microbiome and facilitating macrophage polarization during inflammation. These findings suggest modulation of the commensal wound microbiome using medicinal plants as a potential therapeutic strategy for diabetic wounds.
UR  - https://doi.org/10.1093/burnst/tkae037
DO  - 10.1093/burnst/tkae037