@article{YANG2026, 
author = {Xuanxuan YANG and Dong YANG and Xinru PANG and Danyang SHI and Tianjiao CHEN and Min JIN and Zhiyong HU and Junwen LI},
title = {A real-time quantitative PCR method for detection of viruses in water of the Yellow River basin},
year = {2026},
journal = {Military Medical Sciences},
volume = {50},
number = {6},
pages = {434-440},
keywords = {the Yellow River basin, water environment, viruses, real-time quantitative PCR},
url = {https://www.sciopen.com/article/10.7644/j.issn.1674-9960.2025-00255},
doi = {10.7644/j.issn.1674-9960.2025-00255},
abstract = {ObjectiveTo establish a real-time quantitative PCR (qPCR) method for detection of common viruses in a water environment, and to assess the severity of contamination by norovirus, enterovirus, hepatitis A virus, and human adenovirus F in the Yellow River basin.MethodsA qPCR method for detection of common viruses was established. Viruses were enriched via adsorption-elution, concentrated via ultrafiltration, and quantified by qPCR.ResultsTwenty-two samples were collected during the high-flow season (September-October 2024) and the low-flow season (March 2025). Neither norovirus nor enterovirus was detected in the samples. Hepatitis A virus was detected in 27.27% of the samples, and human adenovirus F in 18.18%. The concentrations of the detected viruses ranged from 1.3 to 2.1 lgGC/L.ConclusionNorovirus and enterovirus are not detected while contamination by hepatitis A virus and human adenovirus F is observed in some samples. These results may provide a reference for biosafety assessment and management of water resources in the Yellow River basin.}
}